• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of attenuation system for autophagy by phosphatases

Research Project

Project/Area Number 25460063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionTokyo Institute of Technology

Principal Investigator

Araki Yasuhiro  東京工業大学, フロンティア研究機構, 特任助教 (60345254)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsオートファジー / リン酸化 / キナーゼ / ホスファターゼ / 蛋白質分解 / 酵母 / 出芽酵母 / ホスファーゼ / タンパク質分解系
Outline of Final Research Achievements

Autophagy is a conserved eukaryotic process of vacuolar/lysosomal-mediated degradation targeting proteins and organelles. Autophagy has to be tightly regulated in order to prevent its occurring at insufficient or excess levels, both of which are harmful for cells. So far, the regulation of autophagy by phosphorylation catalyzed by kinases have been primarily investigated; however, the importance of its reverse reaction, catalyzed by phosphatases, needs to be elucidated.
In this study, I demonstrate that two phosphatases, PP2A and calcineurin, are required for dephosphorylation of Atg13 upon autophagy induction and that Msg5 and Sdp1 dephosphorylate Slt2 kinase to suppress autophagy. In addition, I identified a novel component of the PI3 kinase complex, Atg38. Atg38 functions as a physical linkage between the Vps15-Vps34 and Atg14-Vps30 sub-complexes to facilitate complex I formation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase complex integrity2013

    • Author(s)
      Araki Y, Ku W-C, Akioka M, May AI, Hayashi Y, Arisaka F, Ishihama Y, Ohsumi Y.
    • Journal Title

      J. Cell Biol

      Volume: 203(2) Issue: 2 Pages: 299-313

    • DOI

      10.1083/jcb.201304123

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Phosphorylation of Vps34 by Atg1 is required for autophagy2015

    • Author(s)
      荒木 保弘
    • Organizer
      The 7th International Symposium on autophagy
    • Place of Presentation
      The smoky Willow Resort, Hangshan, Anhui, China
    • Year and Date
      2015-03-19 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] PI3キナーゼの複合体特異的機能、構成因子と制御2014

    • Author(s)
      荒木 保弘
    • Organizer
      第37回 日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜 神奈川県
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] オートファジーに必須なAtg1キナーゼの基質同定2014

    • Author(s)
      荒木 保弘
    • Organizer
      第8回オートファジー研究会
    • Place of Presentation
      シャトレーゼキングダムサッポロ 北海道
    • Year and Date
      2014-11-09 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] PI3キナーゼ複合体のオートファジー特異的リン酸化制御2014

    • Author(s)
      荒木 保弘
    • Organizer
      酵母遺伝学フォーラム第47回研究報告会
    • Place of Presentation
      東京大学弥生講堂 東京都
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Research-status Report
  • [Presentation] A NOVEL COMPONENT OF AUTOPHAGY-SPECIFIC PHOSPHATIDYLINOSITOL 3-KINASE COMPLEX

    • Author(s)
      荒木 保弘
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(兵庫県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Atg38はオートファジー特異的PI3キナーゼ複合体の構成因子である

    • Author(s)
      荒木 保弘
    • Organizer
      第7回オートファジー研究会
    • Place of Presentation
      ヤマハリゾートつま恋(静岡県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Atg38はオートファジー特異的PI3キナーゼ複合体の構成因子である

    • Author(s)
      荒木 保弘
    • Organizer
      酵母遺伝学フォーラム第46回研究報告会
    • Place of Presentation
      東北学院大学(宮城県)
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi