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Molecular mechanism of Myosin1E-mediated cell motility response

Research Project

Project/Area Number 25460067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionNagasaki University

Principal Investigator

TANIMURA Susumu  長崎大学, 医歯薬学総合研究科(薬学系), 助教 (90343342)

Co-Investigator(Kenkyū-buntansha) TAKEDA Kohsuke  長崎大学, 医歯薬学総合研究科(薬学系), 教授 (10313230)
Research Collaborator ARICHIKA Naoya  
HAMAMATSU Ayako  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミオシン / カベオラ / リン酸化 / 細胞運動 / 癌 / Caveolae / Myosin
Outline of Final Research Achievements

We have shown that SH3P2, a negative regulator of cell migration, interacts with Myosin1E (Myo1E) and prevents the localization of Myo1E to the lamellipodial tips. Dissociation of Myo1E from SH3P2 results in the induction of cell motility; however, the molecular mechanism of which still remains unknown. Here, we show that the SH3 domain of Myo1E bound to the Cavin1/3-Caveolin1 complex via the proline-rich region of Cavin3. RNAi-mediated depletion of Myo1E or Cavin3 suppressed the internalization of the Cavin1/3-Caveolin1 complex, the localized activation of Akt, and cell motility. We also revealed that Hsp90 co-chaperone proteins, UNC45 and STIP1, also interact with Myo1E and regulate the localization and expression level of Myo1E, respectively. These results suggest that the function of Myo1E is regulated by SH3P2, UNC45 and STIP1 and that Myo1E induces the localized activation of Akt through the Caveolae regulation and thus cell motility.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results)

  • [Journal Article] Targeting the ERK signaling pathway as a potential treatment for insulin resistance and type 2 diabetes2016

    • Author(s)
      Ozaki K, Awazu M, Tamiya M, Iwasaki Y, Harada A, Kugisaki S, Tanimura S, Kohno M
    • Journal Title

      Am. J. Physiol. Endocrinol. Metab.

      Volume: 310 Issue: 8 Pages: E643-E651

    • DOI

      10.1152/ajpendo.00445.2015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TNF-α induces caspase-1 activation independently of simultaneously induced NLRP3 in 3T3-L1 cells.2016

    • Author(s)
      Furuoka M, Ozaki K, Sadatomi D, Mamiya S, Yonezawa T, Tanimura S, Takeda K.
    • Journal Title

      J. Cell Physiol.

      Volume: in press Issue: 12 Pages: 1-7

    • DOI

      10.1002/jcp.25385

    • NAID

      120006987671

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] SH3P2はMyosin 1Eの細胞質アンカーとして機能する2015

    • Author(s)
      有近 直也、武田 弘資、河野 通明、谷村 進
    • Organizer
      BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会・合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Myosin 1Eはカベオラの極性化を誘導することで細胞運動を亢進させる2015

    • Author(s)
      谷村 進、有近 直也、大山 要、河野 通明、武田 弘資
    • Organizer
      BMB2015(第38回日本分子生物学会年会・第88回日本生化学会大会・合同大会)
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微小管重合阻害はGEF-H1-RhoA-ROCK経路の活性化を介してマイクロベジクルの放出を促進する2015

    • Author(s)
      谷村進、武田弘資
    • Organizer
      第74回 日本癌学会総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Myosin 1EによるCaveolin1の極性化は間葉系様がん細胞のERK経路依存的な細胞運動を亢進させる2014

    • Author(s)
      谷村 進、浜松絢子、大山要、木原康孝、武田弘資、河野通明
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Myosin 1E induces cell motility through localized activation of Akt and S6K2014

    • Author(s)
      谷村 進、武田弘資、河野通明
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Myosin 1EにはAktの局所的な活性化を介して細胞運動を亢進する2014

    • Author(s)
      浜松絢子、木原康孝、河野通明、武田弘資、谷村 進
    • Organizer
      平成26年度日本生化学会九州支部例会
    • Place of Presentation
      九州大学病院キャンパス(福岡市東区)
    • Year and Date
      2014-05-17 – 2014-05-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Myosin1E-SH3P2複合体による細胞運動制御の分子機構2013

    • Author(s)
      谷村進、木原康孝、大山要、中原康子、平田弦也、松丸由美、武田弘資、河野通明
    • Organizer
      平成25年度日本生化学会九州支部例会
    • Place of Presentation
      佐賀県佐賀市本庄町1 佐賀大学本庄キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Myosin1EはCaveolin1-Cavin1/3複合体の安定化を介して細胞運動を促進する2013

    • Author(s)
      谷村進、浜松絢子、木原康孝、大山要、武田弘資、河野通明
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      神奈川県横浜市西区みなとみらい1-1-1 パシフィコ横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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