Genetic analysis of the intestinal granular coordination in C. elegans.

• Project/Area Number: 25460071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: Ohashi-Kobayashi Ayako 岩手医科大学, 薬学部, 教授 (90272484)
• Co-Investigator(Renkei-kenkyūsha): SHIRAISHI Hirohisa 岩手医科大学, 薬学部, 講師 (80393156) ; TANJI Takahiro 岩手医科大学, 薬学部, 助教 (60453320) ; NISHIKORI Kenji 岩手医科大学, 薬学部, 助教 (20563844)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: オルガネラ連携 / 腸内顆粒 / 線虫 / 網羅的スクリーニング / RNAi / 脂肪滴 / ABC輸送体 / 細胞小器官 / 連携 / 遺伝子スクリーニング / 腸 / 小胞輸送 / 薬学 / オルガネラ / 細胞機能

Outline of Final Research Achievements

The genetic factors involved in the coordination and regulation of intestinal granular organelles were investigated, using the soil nematode C.elegans. For comprehensive genetic analysis, we performed feeding RNAi screening, which is popular in C. elegans. Out of 11,479 independent clones, 142 clones displayed the significant decrease of major large gut granules in the worms. Some of these clones also showed the tremendous accumulation of birefringent granules, which is minor in normal worms. These results would be a foundation for further understanding the coordination of various intestinal granules and other organelles.

Research Products

• [Journal Article] Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells. (2016)
  • Author(s): Tanji T, Nishikori K, Haga S, Kanno Y, Kobayashi Y, Takaya M, Gengyo-Ando K, Mitani S, Shiraishi H, Ohashi-Kobayashi A.
  • Journal Title: BMC Cell Biology Volume: 17 Issue: 1 Pages: 4-4
  • DOI: 10.1186/s12860-015-0076-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intracellular localization of ABC transporter TAPL differs between transient and stable expression. (2014)
  • Author(s): Y. Motohashi, A. Ohashi-Kobayashi, M. Nakanishi-Matsui, Y. Fujimoto, and M. Maeda
  • Journal Title: Cellbio Volume: 3 Issue: 02 Pages: 50-59
  • DOI: 10.4236/cellbio.2014.32006
  • Peer Reviewed
• [Journal Article] Amino-terminal extension of 146 residues of L-type GATA-6 is required for transcriptional activation but not for self-association. (2014)
  • Author(s): K. Takada, K. Obayashi, K. Ohashi, A. Kobayashi-Ohashi, M. Nakanishi-Matsui, and M. Maeda
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 452 Issue: 4 Pages: 962-966
  • DOI: 10.1016/j.bbrc.2014.09.019
  • Peer Reviewed
• [Journal Article] Cooperative function and mutual stabilization of the half ATP-binding cassette transporters HAF-4 and HAF-9 in Caenorhabditis elegans. (2013)
  • Author(s): Tanji T, Nishikori K, Shiraishi H,Maeda M, Ohashi-Kobayashi A.
  • Journal Title: Biochem. J. Volume: (in press) Issue: 3 Pages: 467-475
  • DOI: 10.1042/bj20130115
  • Peer Reviewed
• [Presentation] 線虫腸細胞内顆粒状オルガネラの微細構造の加齢変化 (2015)
  • Author(s): 白石博久、鈴木成惇、梶田太一、錦織健児、丹治貴博、大橋綾子
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 線虫における cdc-48 遺伝子のノックダウンは、腸細胞内の複屈折性物質の蓄積をもたらす (2015)
  • Author(s): 丹治貴博、白石博久、錦織健児、田邉麻莉、田代翔平、佐藤祐紀、髙橋あかね、大橋綾子
  • Organizer: BMB2015
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 線虫腸細胞の加齢応答性オルガネラの解析に向けたゲノム編集技術導入の試み (2015)
  • Author(s): 宇部和成、錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 矢巾, 岩手
  • Year and Date: 2015-09-26
• [Presentation] 線虫腸細胞オルガネラHEBE顆粒の形態に餌として与える細菌種が及ぼす影響 (2015)
  • Author(s): 鈴木俊介、丹治貴博、錦織健児、白石博久、大橋綾子
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 矢巾, 岩手
  • Year and Date: 2015-09-26
• [Presentation] 加齢及び絶食時に線虫腸細胞内に見られるHEBE顆粒の消失と、それに伴うペプチド輸送体ホモログ（HAF-4、HAF-9）局在の変化 (2015)
  • Author(s): 濵田佳男、錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 矢巾, 岩手
  • Year and Date: 2015-09-26
• [Presentation] Characterization of HEBE granules, novel organelles found in the intestine of well-fed young adult C. elegans. (2015)
  • Author(s): Takahiro Tanji, Kenji Nishikori, Syoko Haga, Yusuke Kobayashi, Yuki Kanno, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi
  • Organizer: 20th International C. elegans Meeting
  • Place of Presentation: ロサンゼルス、米国
  • Year and Date: 2015-06-27
  • Int'l Joint Research
• [Presentation] Genes involved in proton transport are required for the formation of HEBE granule, which is a novel aging- and fasting-responsive organelle (2015)
  • Author(s): Kenji Nishikori, Rei Takahashi, Mami Sato, Takahiro Tanji, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi
  • Organizer: 20th International C. elegans Meeting
  • Place of Presentation: ロサンゼルス、米国
  • Year and Date: 2015-06-26
  • Int'l Joint Research
• [Presentation] Characterization of lysosomal peptide transporters and their localizing intracellular organelles in Caenorhabditis elegans. (2015)
  • Author(s): Hirohisa Shiraishi, Takahiro Tanji, Kenji Nishikori, and Ayako Ohashi-Kobayashi
  • Organizer: BIT’s 8th PepCon Annual World Protein & Peptide Conference 2015
  • Place of Presentation: 南京, 中国
  • Year and Date: 2015-04-27
  • Int'l Joint Research / Invited
• [Presentation] 腸の若さの指標としてのHEBE顆粒の可能性～線虫の老化と健康寿命に着目して～ (2015)
  • Author(s): 丹治貴博、錦織健児、白石博久、大橋綾子
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（神戸）
  • Year and Date: 2015-03-28
• [Presentation] Age- and nutrition-associated change in the morphology and population of intestinal granules in Caenorhabditis elegans. (2014)
  • Author(s): Hirohisa Shiraishi, Maya Kamikawara, Seijun Suzuki, Eri Matsuura, Katsutoshi Ogasawara, Kinji Ishida, Koujiro Tohyama, Kenji Nishikori, Takahiro Tanji, Ayako Ohashi-Kobayashi.
  • Organizer: C. elegans Development, Cell Biology and Gene Expression Meeting in association with the 6th Asia-Pacific C. elegans Meeting
  • Place of Presentation: 奈良県新公会堂（奈良）
  • Year and Date: 2014-07-26
• [Presentation] DAF-16 and DAF-18 are required for the normal formation and/or maintenance of the specific intestinal organelles in C. elegans.（C. elegans Topics Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs (2014)
  • Author(s): Takahiro Tanji, Kenji Nishikori, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi.
  • Organizer: C. elegans Topics Meeting: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs
  • Place of Presentation: ウィスコンシン州マディソン(米国)
  • Year and Date: 2014-07-12
• [Presentation] 抗原ペプチド輸送体様タンパク質が局在する線虫新規オルガネラ (2014)
  • Author(s): 錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 日本比較免学会第26回学術集会、第25回日本生体防御学会学術集会合同大会
  • Place of Presentation: 東北大学（仙台）
  • Year and Date: 2014-07-10
• [Presentation] 飢餓および加齢に伴う線虫腸細胞内膜構造の動的変動の観察 (2014)
  • Author(s): 上川原麻弥、鈴木成惇、錦織健児、丹治貴博、松浦絵里、小笠原勝利、石田欣二、遠山稿二郎、白石博久、大橋綾子
  • Organizer: 日本生化学会東北支部 第80回例会・シンポジウム
  • Place of Presentation: 秋田パークホテル（秋田）
  • Year and Date: 2014-05-10
• [Presentation] Monitoring of age-dependent alteration of C. elegans intestinal granules by organelle staining dyes and marker proteins.
  • Author(s): Yusuke Kobayashi, Hiroyuki Hayashi, Yuki Ueda, Yuki Kanno, Syoko Haga,Kenji Nishikori, Takahiro Tanji, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi
  • Organizer: FAOBMB Mini Symposium 2013
  • Place of Presentation: 岩手医科大学矢巾キャンパス
• [Presentation] Study on nutrient component required for the formation and maintenance of intestinal granules in C. elegans.
  • Author(s): Mio Asanuma, Eisuke Kuroda, Takahiro Tanji, Kenji Nishikori, Hirohisa,Shiraishi, Ayako Ohashi-Kobayashi
  • Organizer: FAOBMB Mini Symposium 2013
  • Place of Presentation: 岩手医科大学矢巾キャンパス
• [Presentation] Exploration and analysis of genetic factors involved in the formation of intestinal granules in C. elegans via RNAi method.
  • Author(s): Maya Ishibashi, Shohei Tashiro, Mari Tanabe, Yumi Asanuma, Takato Oikawa,Kenji Nishikori, Takahiro Tanji, Hirohisa Shiraishi, Ayako Ohashi–Kobayashi
  • Organizer: FAOBMB Mini Symposium 2013
  • Place of Presentation: 岩手医科大学矢巾キャンパス
• [Presentation] The discovery of intestinal intracellular microbes in the soil nematode collected from the field of the Tohoku district, northeastern Japan.
  • Author(s): Kenji Nishikori, Eisuke Kuroda, Takahiro Tanji, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: UCLA ロサンゼルス, 米国
• [Presentation] Genetic requirements of the pentose phosphate pathway for the intestinal granule formation in C. elegans.
  • Author(s): Hirohisa Shiraishi, Takato Oikawa, Maya Ishibashi, Mari Tanabe, Yumi Asanuma, Reiko Aoyama, Kenji Nishikori, Takahiro Tanji, Ayako Ohashi-Kobayashi.
  • Organizer: 19th International C. elegans Meeting
  • Place of Presentation: UCLA ロサンゼルス, 米国
• [Presentation] Age-associated replacement of the major C. elegans intestinal granules by fat storage organelles during adult stage.
  • Author(s): Yuki Kanno, Yusuke Kobayashi, Hiroyuki Hayashi, YukiUeda, Takahiro Tanji, Kenji Nishikori, Hirohisa Shiraishi, AyakoOhashi-Kobayashi.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Starvation-induced selective decrease of intestinal organelles in Caenorhabditis elegans.
  • Author(s): Takahiro Tanji, Kenji Nishikori, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Classification of Caenorhabsitis elegans intestinal granules using marker proteins.
  • Author(s): Syoko Haga, Yusuke Kobayashi, Takahiro Tanji, Kenji Nishikori, Hirohisa Shiraishi, Ayako Ohashi-Kobayashi.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 線虫腸細胞に見られる顆粒状オルガネラの形成に関わる遺伝的要因の解析～V-ATPase を構成する遺伝子の関与～
  • Author(s): 高橋怜衣、西舘佳祐、錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 東北大学
• [Presentation] 加齢や栄養環境に依存した線虫腸細胞オルガネラのポピュレーション変化
  • Author(s): 髙谷麻衣、立花繭子、官野祐季、丹治貴博、錦織健児、白石博久、大橋綾子
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 東北大学
• [Presentation] 線虫腸細胞で見出された新奇オルガネラ“HEBE顆粒”
  • Author(s): 丹治貴博、錦織健児、白石博久、大橋綾子
  • Organizer: 第52回日本薬学会東北支部大会
  • Place of Presentation: 東北大学
• [Presentation] 線虫腸細胞の新奇オルガネラHEBE顆粒
  • Author(s): 大橋綾子、丹治貴博、錦織健児、白石博久
  • Organizer: 岩手医科大学バイオイメージングセミナー55周年記念セミナー
  • Place of Presentation: 岩手医科大学矢巾キャンパス
  • Invited
• [Book] スタンダード薬学シリーズII 第４巻 生命現象の基礎 (2015)
  • Author(s): 奥直人他（代表編者）、大橋綾子他（分担執筆）
  • Total Pages: 359
  • Publisher: 東京化学同人
• [Book] コンパス生化学 (2015)
  • Author(s): 前田正知、浅野真司（編者）中西真弓、大橋一晶、大橋綾子他（分担執筆）
  • Total Pages: 426
  • Publisher: 南江堂
• [Book] 廣川書店 (2013)
  • Author(s): 伊東晃他 (分担執筆）
  • Total Pages: 339
  • Publisher: 薬学領域の生化学（第２版）
• [Remarks] WormBase GENE HAF-4
  • URL: http://www.wormbase.org/species/c_elegans/gene/WBGene00001814#0c-9ge-3
• [Remarks] WormBase GENE HAF-9
  • URL: http://www.wormbase.org/species/c_elegans/gene/WBGene00001819#0c-9ge-3
• [Remarks] 生体防御学講座ホームページ
  • URL: http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42
• [Remarks] 岩手医科大学薬学部生体防御学講座ホームページ
  • URL: http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42
• [Remarks] WormBase GENE Haf-4
  • URL: http://www.wormbase.org/species/c_elegans/gene/WBGene00001814#0b-9d-4
• [Remarks] WormBase GENE Haf-9
  • URL: http://www.wormbase.org/species/c_elegans/gene/WBGene00001819#0b-9d-4