Analysis of the onset mechanism of myeloproliferative neoplasms by the quantitative phosphorylation proteome analysis
Project/Area Number |
25460073
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Keio University |
Principal Investigator |
TAGO MEGUMI 慶應義塾大学, 薬学部, 准教授 (30445192)
|
Co-Investigator(Renkei-kenkyūsha) |
SUGIYAMA NAOYUKI 京都大学, 薬学部, 准教授 (50545704)
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Research Collaborator |
Ueda Fumihito
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | JAK2V617F変異体 / 定量的リン酸化プロテオーム解析 / 慢性骨髄増殖性腫瘍 / エリスロポエチン受容体 / DDX5 / JAK2 / CIS / リン酸化プロテオーム解析 / プロテオーム / シグナル伝達 |
Outline of Final Research Achievements |
A somatic mutation (V617F) in tyrosine kinase JAK2 was found in the majority of myeloproliferative neoplasm (MPN) patients. It has been shown that the JAK2 V617F mutant was constitutively active and induced the cytokine-independent cell proliferation and tumorigenesis, suggesting that it behaves as a potent oncogene product. However, the molecular mechanism how JAK2 V617F mutant induces cellular transformation has not been elucidated. To clarify the molecular mechanism of JAK2 V617F mutant-induced transformation, it is necessary to analyze the states of all signal molecules at the downstream of JAK2 V617F mutant at the same time. Therefore, we performed quantitative phosphoproteome analysis to identify the phosphorylated proteins at downstream of JAK2 V617F mutant and analyze the roles of these molecules.
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Report
(4 results)
Research Products
(48 results)
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[Presentation] コーヒー豆抽出液が示す抗炎症作用の検討2016
Author(s)
谷尾 真由美, 野中 勇佑, 武田 峰佳, 八木 利恭,古旗 賢二, 多胡 憲治, 多胡 めぐみ, 田村 悦臣
Organizer
日本薬学会第136年会(横浜)
Place of Presentation
パシフィコ横浜(神奈川県・横浜市)
Year and Date
2016-03-29
Related Report
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[Presentation] コーヒーによる白内障抑制効果の検討2016
Author(s)
石森 奈奈, 中澤 洋介, 多胡 めぐみ, 岡 美佳子,竹鼻 眞, 田村 悦臣
Organizer
日本薬学会第136年会(横浜)
Place of Presentation
パシフィコ横浜(神奈川県・横浜市)
Year and Date
2016-03-29
Related Report
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