Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Outline of Final Research Achievements |
We explored functional roles of miR-S1 encoded by SV40 virus in DNA replication and cytokine expression within HEK293 cells transfected with SV40 genome vectors. Semi-quantitative qPCR analysis showed that miR-S1-3p rather than miR-S1-5p was more abundantly expressed in cells transfected with SV40 genome vectors. Consistently, reporter assays showed that miR-S1-3p had much more repressive effects on luciferase activity than miR-S1-5p did. Replication rate of viral DNA were evaluated by qPCR analysis in various types of cells transfected with SV40 vectors with or without miR-S1 expression. These results indicated that the replication rate increased proportionally to T antigen expression, but was retarded by its excess expression, often observed in transfected cells without miR-S1 expression. Examination of cytokine expressions in HEK293 cells transfected with SV40 vectors revealed that miR-S1 affected the expression of TNFalpha and IL17F through its modulation of T antigen expression.
|