The roles of uPA/uPAR on the inflammatory osteoclastogenesis

• Project/Area Number: 25460078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Doshisha Women's College of Liberal Arts
• Principal Investigator: KANNO YOSUKE 同志社女子大学, 薬学部, 助教 (40416178)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: uPAR / uPA / LPS / osteoclast / bone / osteoclasts / inflammation

Outline of Final Research Achievements

The aim of this study was to clarify the role of uPA/uPAR on the inflammatory bone loss. We found that uPA attenuated inflammatory osteoclastogenesis through the activation of AMPK. Additionally, we found that uPAR regulated inflammatory osteoclastogenesis through the integrin/Akt pathway. Moreover, we found that uPAR regulated inflammation-induced TNF-α production. These data suggest that uPA/uPAR plays an important roles on the inflammatory bone loss.

Research Products

• [Journal Article] uPA attenuated LPS-induced inflammatory osteoclastogenesis through the plasmin/PAR-1/Ca2+/CaMKK/AMPK axis. (2016)
  • Author(s): Kanno Y, Ishisaki A, Kawashita E, Kuretake H, Ikeda K, Matsuo O.
  • Journal Title: Int J Biol Sci. Volume: 12 Issue: 1 Pages: 63-71
  • DOI: 10.7150/ijbs.12690
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] α2AP mediated myofibroblast formation and the development of renal fibrosis in unilateral ureteral obstruction (2014)
  • Author(s): Yosuke Kanno, Eri Kawashita, Akiko Kokado, Hiromi Kuretake, Kanako Ikeda, Kiyotaka Okada, Mariko Seishima, Shigeru Ueshima, Osamu Matsuo, Hiroyuki Matsuno
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 5967-5967
  • DOI: 10.1038/srep05967
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 27. Involvement of alpha2-antiplasmin in dendritic growth in hippocampal neurons (2013)
  • Author(s): Eri Kawashita, Yosuke Kanno, Haruka Asayama, Kiyotaka Okada, Shigeru Ueshima, Osamu Matsuo, Hiroyuki Matsuno
  • Journal Title: Journal of Neurochemistry Volume: 126 Issue: 1 Pages: 58-69
  • DOI: 10.1111/jnc.12281
  • Peer Reviewed
• [Journal Article] Cilostazol attenuates spontaneous microaggregation of platelets in type 2 diabetic patients with insufficient platelet response to aspirin. (2013)
  • Author(s): Araki S, Matsuno H, Haneda M, Koya D, Kanno Y, Kume S, Isshiki K, Araki H, Ugi S, Kawai H, Kashiwagi A, Uzu T, Maegawa H.
  • Journal Title: Diabetes Care Volume: 36(7) Issue: 7 Pages: e92-e93
  • DOI: 10.2337/dc12-2702
  • Peer Reviewed
• [Presentation] 骨代謝におけるalpha2-antiplasminの役割 (2015)
  • Author(s): 呉竹 紘実, 菅野 陽介, 河下 映里, 池田 夏菜子, 松尾 理
  • Organizer: 日本薬学会 第１３５年会
  • Place of Presentation: 神戸学院大学
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 骨代謝におけるalpha2-antiplasminの役割 (2014)
  • Author(s): 呉竹紘実、河下映里、池田夏菜子、菅野陽介
  • Organizer: 第６４回 日本薬学会近畿支部総会・大会
  • Place of Presentation: 京都薬科大学
  • Year and Date: 2014-10-11
• [Presentation] 線維化病態の発症・進行におけるalpha2-antiplasminの役割 (2014)
  • Author(s): 菅野陽介
  • Organizer: 生体機能と創薬シンポジウム
  • Place of Presentation: 近畿大学薬学部
  • Year and Date: 2014-08-28 – 2014-08-29
  • Invited
• [Presentation] The role of alpah2AP in the development of renal interstitial fibrosis. (2013)
  • Author(s): Y Kanno, A Kokado, E Kawashita, M Tani, K Okada, S Ueshima, O Matsuo, H Matsuno
  • Organizer: XXIV Congress of the international society on thrombosis and hamostasis
  • Place of Presentation: オランダ アムステルダム
• [Presentation] The role of plasmin(ogen) in the pathogenesis of inflammatory bowel diseases. (2013)
  • Author(s): S Inaba, Ykanno, E Kawashita, K Okada, S Ueshima, O Matsuo, H Matsuno
  • Organizer: XXIV Congress of the international society on thrombosis and hamostasis
  • Place of Presentation: オランダ アムステルダム
• [Presentation] Alpha2-antiplasmin is a potential regulator of neuronal morphology. (2013)
  • Author(s): E Kawashita, Y Kanno, H Asayama, K Okada, S Ueshima, O Matsuo, H Matsuno
  • Organizer: XXIV Congress of the international society on thrombosis and hamostasis
  • Place of Presentation: オランダ アムステルダム
• [Presentation] A role of Cilostazol in Alzheimer’s disease treatment: Induction of LRP1 expression in endothelial cells. (2013)
  • Author(s): Y Yano, C Takeshima, E Kawashita, Y Kanno, H Matsuno
  • Organizer: XXIV Congress of the international society on thrombosis and hamostasis
  • Place of Presentation: オランダ アムステルダム
• [Presentation] The role of EXT in the pathogenesis of vascular complications of diabetes. (2013)
  • Author(s): N Wakabayashi, M Kimura, Y Kanno, E Kawashita, H Matsuno
  • Organizer: XXIV Congress of the international society on thrombosis and hamostasis
  • Place of Presentation: オランダ アムステルダム
• [Presentation] 糖尿病性アルツハイマー病発症におけるエンドトキシンの関与Endotoxin induces the pathogenesis of Alzheimer's disease related to diabetes mellitus (2013)
  • Author(s): 矢野 裕子, 河下 映里, 菅野 陽介, 茸谷 麻帆, 松野 浩之
  • Organizer: 第８６回日本生化学会大会
  • Place of Presentation: 横浜
• [Remarks] 同志社女子大学 研究者データベース
  • URL: http://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/2842/2842_Researcher.html
• [Remarks] 同志社女子大学 研究者データベース
  • URL: http://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/2842/2842_Researcher.html