| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
During mitosis, cyclin A and cyclin B are ubiquitylated by the anaphase promoting complex/cyclosome (APC/C) and then subjected to proteasomal degradation. However, cyclin A, but not cyclin B, begins to be degraded in the prometaphase when APC/C is inactivated by the spindle assembly checkpoint (SAC). Here we show that Apollon (also known as BRUCE or BIRC6) plays a role in SAC-independent degradation of cyclin A in early mitosis. Apollon interacts with cyclin A and facilitates cyclin A ubiquitylation. In Apollon-deficient cells, mitotic degradation of cyclin A is delayed, and total but not CDK-bound cyclin A level was increased. We propose Apollon to be a novel regulator of mitotic cyclin A degradation independent on SAC.
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