Identification of regulatory factors for TMEM16A calcium-activated chloride channel
| Project/Area Number |
25460104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pharmacology in pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
YAMAMURA Hisao 名古屋市立大学, 薬学研究科(研究院), 准教授 (80398362)
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| Co-Investigator(Renkei-kenkyūsha) |
IMAIZUMI Yuji 名古屋市立大学, 大学院薬学研究科, 教授 (60117794)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | クロライドチャネル / カルシウム / TMEM16 / 血管 / 平滑筋 / 門脈 / 門脈圧亢進症 / イオンチャネル / TMEM16A / 肝硬変 / 胆管結紮モデル / イメージング / アクチン / スプライスバリアント |
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| Outline of Final Research Achievements |
The calcium-activated chloride (ClCa) channel plays substantial roles in the regulation of membrane excitability in vascular smooth muscles. Recently, TMEM16A-coding protein has been identified as the molecular entity responsible for ClCa channel in several types of vascular smooth muscles. In this study, the functional expression of TMEM16A and its regulatory factors was examined in murine portal vein smooth muscle cells. TMEM16A was abundantly expressed in portal vein myocytes and formed a dimeric ClCa channel. The activity of TMEM16A ClCa channel was modified by the interaction with actin cytoskeleton. TMEM16A was also interacted with TMEM16B to form a heteromeric ClCa channel. Finally, TMEM16A was downregulated in portal vein smooth muscle cells from hepatic cirrhosis-induced portal hypertensive mice. These results provide useful information for elucidating physiological and pathological significances of TMEM16A ClCa channels in vascular smooth muscles.
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Report
(4 results)
Research Products
(142 results)
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[Journal Article] Pathogenic role of calcium-sensing receptors in the development and progression of pulmonary hypertension.2016
Author(s)
Tang H, Yamamura A, Yamamura H, Song S, Fraidenburg DR, Chen J, Gu Y, Pohl NM, Zhou T, Jiménez-Pérez L, Ayon RJ, Desai AA, Goltzman D, Rischard F, Khalpey ZI, Black SM, Garcia JG, Makino A, Yuan JX.
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Journal Title
Am J Physiol Lung Cell Mol Physiol.
Volume: in press
Issue: 9
Pages: L846-L859
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Notch Activation of Ca2+ Signaling Mediates Hypoxic Pulmonary Vasoconstriction and Pulmonary Hypertension2015
Author(s)
Smith KA, Voiriot G, Tang H, Fraidenburg DR, Song S, Yamamura H, Yamamura A, Guo Q, Wan J, Pohl NM, Tauseef M, Bodmer R, Ocorr K, Thistlethwaite PA, Haddad GG, Powell FL, Makino A, Mehta D, Yuan JX
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Journal Title
Am J Respir Cell Mol Biol
Volume: in press
Issue: 3
Pages: 355-367
DOI
Related Report
Peer Reviewed
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[Journal Article] Up-regulation of KCa3.1 K^+ channel in mesenteric lymph node CD4^+ T-lymphocytes from a mouse model of dextran sodium sulfate-induced inflammatory bowel disease2014
Author(s)
Ohya S, Fukuyo Y, Kito H, Shibaoka R, Matsui M, Niguma H, Maeda Y, Yamamura H, Fujii M, Kimura K, Imaizumi Y
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Journal Title
Am J Physiol Gastrointest Liver Physiol
Volume: in press
Issue: 10
Pages: G873-G885
DOI
Related Report
Peer Reviewed
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[Presentation] Enhanced Ca2+-sensing receptor function in idiopathic pulmonary arterial hypertension
Author(s)
Aya Yamamura, Qiang Guo, Hisao Yamamura, Adriana M. Zimnicka, Nicole M. Pohl, Kimberly A. Smith, Ruby A. Fernandez1, Amy Zeifman, Ayako Makino, Hui Dong, Jason X.-J. Yuan
Organizer
IUPS2013
Place of Presentation
Birmingham, UK
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