| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The calcium-activated chloride (ClCa) channel plays substantial roles in the regulation of membrane excitability in vascular smooth muscles. Recently, TMEM16A-coding protein has been identified as the molecular entity responsible for ClCa channel in several types of vascular smooth muscles. In this study, the functional expression of TMEM16A and its regulatory factors was examined in murine portal vein smooth muscle cells. TMEM16A was abundantly expressed in portal vein myocytes and formed a dimeric ClCa channel. The activity of TMEM16A ClCa channel was modified by the interaction with actin cytoskeleton. TMEM16A was also interacted with TMEM16B to form a heteromeric ClCa channel. Finally, TMEM16A was downregulated in portal vein smooth muscle cells from hepatic cirrhosis-induced portal hypertensive mice. These results provide useful information for elucidating physiological and pathological significances of TMEM16A ClCa channels in vascular smooth muscles.
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