Search for a lead compound based on antiausteric activity
| Project/Area Number |
25460117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Hokuriku University (2014-2016)
University of Toyama (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 膵臓がん / 栄養飢餓耐性解除 / 和漢薬 / 活性成分 / 抗がん剤 / 穿心蓮 |
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| Outline of Final Research Achievements |
Preferential cytotoxicity of 24 extracts was determined against PANC-1 and PSN-1 human pancreatic cancer cells and extracts of Andrographis Herba, Isodonis Herba, and Licopodium showed activities. Then, phytochemical investigation of the 70% EtOH extract of Andrographis Herba led to the isolation of 21 known compounds consisting of six labdane-type diterpenes, six flavones, three flavanones, two sterols, a fatty acid, a phthalate, a triterpene, and a monoterpene. Among them, 14-deoxy-11,12-didehydroandrographolide displayed the most potent preferential cytotoxicity against PANC-1 and PSN-1 cells with PC50 values of 10.0 microM and 9.27 microM, respectively. Microscopical observation, double staining with ethidium bromide (EB) and acridine orange (AO), and flow cytometry with propidium iodide/annexin V double staining indicated that 14-deoxy-11,12-didehydroandrographolide triggered apoptosis-like cell death in NDM with an amino acids and/or serum-sensitive mode.
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Report
(5 results)
Research Products
(3 results)
