| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
Constituents in decoctions of a representative Kampo prescription and related formulations were analyzed quantitatively. HPLC conditions for simultaneous analyses of those constituents with a wide range of polarities including hydrolysable tannins were established. Particularly, we focused on the interactions between the constituents affecting the compositional changes in the constituents. Precipitations in the decoctions were also analyzed using UV-visible spectral analyses. Participation of the electrostatic and hydrophobic interactions in forming the precipitations were suggested. We found that GPC analyses are effective for the behaviors of polymeric polyphenols of rhubarb, a representative crude drug, and precipitations are formed as a result of interactions between those polyphenols of rhubarb and constituents derived from some other crude drugs in respective Kampo prescriptions.
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