Application of D-cycloserine (an anti-tubercular agent) biosynthetic genes to drug production
| Project/Area Number |
25460119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Hiroshima University |
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Principal Investigator |
Kumagai Takanori 広島大学, 医歯薬保健学研究院(薬), 准教授 (70274058)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 抗結核薬 / D-サイクロセリン / 遺伝子破壊 / 代謝工学 / 異種生産 |
|---|
| Outline of Final Research Achievements |
In the present study, high level heterologous production system of an anti-tubercular agent, D-cycloserine (D-CS), by Escherichia (E.) coli was established through co-expression of the four D-CS biosynthetic genes (dcsC, dcsD, dcsE and dcsG) and a self-resistance gene, dcsJ, in combination with metabolic engineering of the E. coli host. In addition, enzymatic functions of DcsA and DcsG involved in the D-CS biosynthesis were clarified by using recombinant proteins expressed in E. coli and purified.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Aspergillus oryzae S-03 produces gingipain inhibitors as a virulence factor Porphyromonas gingivalis2014
Author(s)
Danshiitsoodol, N., Yamashita, H., Noda, M., Kumagai, T., Matoba, Y., and Sugiyama, M.
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Journal Title
J. Bacteriol. Virol.
Volume: 44
Pages: 152-161
Related Report
Peer Reviewed
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[Journal Article] Establishment of an in vitroD-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway2013
Author(s)
Uda, N., Matoba, Y., Kumagai, T., Oda, K., Noda, M., and Sugiyama, M
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Journal Title
Antimicrob. Agents Chemother
Volume: 57
Issue: 6
Pages: 2603-2612
DOI
Related Report
Peer Reviewed
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