| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In the present study, high level heterologous production system of an anti-tubercular agent, D-cycloserine (D-CS), by Escherichia (E.) coli was established through co-expression of the four D-CS biosynthetic genes (dcsC, dcsD, dcsE and dcsG) and a self-resistance gene, dcsJ, in combination with metabolic engineering of the E. coli host. In addition, enzymatic functions of DcsA and DcsG involved in the D-CS biosynthesis were clarified by using recombinant proteins expressed in E. coli and purified.
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