Lb. reuteri BM53-1 inhibits the S. mutans biofilm formation through the alteration of gene expressions

• Project/Area Number: 25460121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Hiroshima University
• Principal Investigator: NODA MASAFUMI 広島大学, 医歯薬保健学研究院 (薬), 特任講師 (40457289)
• Co-Investigator(Kenkyū-buntansha): SUGIYAMA Masanori 広島大学, 大学院医歯薬保健学研究院, 教授 (30106801)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: プロバイオティクス / 乳酸菌 / S. mutans / バイオフィルム / 遺伝子破壊ベクター / バイオサーファクタント

Outline of Final Research Achievements

We previously isolated the plant-derived lactic acid bacterium (LAB) strain, designated BM53-1, from catmint (Actinidia polygama) and identified it as Lactobacillus reuteri. The one of the caries causative bacterium Streptococcus mutans forms biofilm, and the BM53-1 strain produces anti biofilm-forming activity compound in culture broth. In the present study, it was found that the anti biofilm compound alternates the gene expression balances of gtf genes, which are necessary to biofilm formation, by using the quantitative PCR method.

Research Products

• [Journal Article] Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived <i>Lactobacillus brevis</i> 174A (2015)
  • Author(s): Noda, M., Miyauchi, R., Danshiitoodol, N., Higashikawa, F., Kumagai, T., Matoba, Y., Sugiyama, M.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 38 Issue: 12 Pages: 1902-1909
  • DOI: 10.1248/bpb.b15-00505
  • NAID: 130005112637
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] くらしとプロバイオティクス. (2015)
  • Author(s): 野田正文
  • Journal Title: たんじゅうさん Volume: 14 Pages: 16-17
• [Journal Article] Oral lactic acid bacteria related to the occurrence and/or progression of dental caries in Japanese preschool children (2015)
  • Author(s): Shimada, A., Noda, M., Matoba, Y., Kumagai, T., Kozai, K., Sugiyama, M.
  • Journal Title: Bioscience of Microbiota, Food and Health Volume: 34 Issue: 2 Pages: 29-36
  • DOI: 10.12938/bmfh.2014-015
  • NAID: 130005066553
  • ISSN: 2186-3342
  • Peer Reviewed / Open Access
• [Presentation] 植物乳酸菌Lactobacillus brevis 174Aの産生する二成分バクテリオシンの特性ならびに産生制御機構の解析 (2015)
  • Author(s): 野田正文, Narandalai Danshiitsoodol, 的場康幸, 熊谷孝則, 東川史子, 杉山政則
  • Organizer: 第54回日本薬学会・日本薬剤師会・日本病院薬剤師会 中国四国支部学術大会
  • Place of Presentation: 高知県高知市
  • Year and Date: 2015-10-31
• [Presentation] 乳酸菌と大腸菌間で複製する温度感受性シャトルベクターの構築 (2015)
  • Author(s): 岡慶人, 熊谷孝則, 井手日奈子, 野田正文, 的場康幸, 杉山政則
  • Organizer: 日本乳酸菌学会2015年度大会
  • Place of Presentation: 千葉県市川市
  • Year and Date: 2015-07-11
• [Presentation] Lactobacillus brevis 174Aの産生する二成分バクテリオシンの特徴とその発現制御機構 (2015)
  • Author(s): 野田正文, 宮内瑠美, Narandalai Danshiitsoodol, 的場康幸, 熊谷孝則, 東川史子, 杉山政則
  • Organizer: 日本乳酸菌学会2015年度大会
  • Place of Presentation: 千葉県市川市
  • Year and Date: 2015-07-11
• [Presentation] 腸内細菌・乳酸菌と健康とのかかわり (2014)
  • Author(s): 野田正文
  • Organizer: 日本消化器病学会中国支部例会 第74回市民公開講座
  • Place of Presentation: 広島
  • Year and Date: 2014-11-30
  • Invited
• [Presentation] 乳幼児口腔内より分離されたLactobacillus属乳酸菌の耐酸性と齲蝕との相関性 (2013)
  • Author(s): 野田正文, 島田歩, 大黒彩加, 的場康幸, 熊谷孝則, 香西克之, 杉山政則
  • Organizer: 日本乳酸菌学会2013年度大会
  • Place of Presentation: 札幌