Screening for antiviral compounds targeting nuclear export system from natural resources

• Project/Area Number: 25460125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Nagasaki University
• Principal Investigator: WATANABE Ken 長崎大学, 医歯薬学総合研究科(医学系), 助教 (00346909)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: インフルエンザウイルス / 阻害剤 / 抗ウイルス薬 / 核外輸送 / NP / 天然資源 / ウイルス / スクリーニング

Outline of Final Research Achievements

Objective of this study is to find novel anti-viral drugs especially anti-influenza viral drugs. Using cell culture and live virus system, we elucidated anti-viral activity of various natural products and chemicals. As a result, we found that many natural products such as food and plant extracts have anti-influenza viral activity with various mechanisms of action.These compounds and extracts may be useful for the development of novel anti viral drugs.

Research Products

• [Journal Article] Identification of Small Molecule Inhibitors for Influenza A Virus Using In Silico and In Vitro Approaches, (2017)
  • Author(s): Makau JN, Watanabe K, Ishikawa T, Mizuta S, Hamada T, Kobayashi N, Nishida N
  • Journal Title: PLoS ONE Volume: 12 Issue: 3 Pages: e0173582-e0173582
  • DOI: 10.1371/journal.pone.0173582
  • NAID: 120006987539
  • Peer Reviewed / Open Access
• [Journal Article] In vitro evaluation of synergistic inhibitory effects of neuraminidase inhibitors and methylglyoxal against influenza virus infection (2015)
  • Author(s): Charyasriwong S, Watanabe K, Rahmasari R, Matsunaga A, Haruyama T, Kobayashi N
  • Journal Title: Archives of Medical Research Volume: 46 Issue: 1 Pages: 8-16
  • DOI: 10.1016/j.arcmed.2014.12.002
  • Peer Reviewed
• [Journal Article] Nuclear export of the influenza virus ribonucleoprotein complex: Interaction of Hsc70 with viral proteins M1 and NS2 (2014)
  • Author(s): Watanabe K, Shimizu T, Noda S, Tsukahara F, Maru, Y, Kobayashi N
  • Journal Title: FEBS Open Bio Volume: 4 Issue: 1 Pages: 683-688
  • DOI: 10.1016/j.fob.2014.07.004
  • NAID: 120006986451
  • Peer Reviewed / Open Access
• [Journal Article] Anti-Influenza viral effects of honey in vitro: potent high activity of manuka honey (2014)
  • Author(s): Watanabe K, Rahmasari R, Matsunaga A, Haruyama T, Kobayashi N
  • Journal Title: Archives of Medical Research Volume: 45 Issue: 5 Pages: 359-365
  • DOI: 10.1016/j.arcmed.2014.05.006
  • Peer Reviewed
• [Journal Article] Anti-influenza activity of <i>Alchemilla mollis</i> extract: Possible virucidal activity against influenza virus particles (2013)
  • Author(s): Makau JN, Watanabe K, Kobayashi N
  • Journal Title: Drug Discoveries & Therapeutics Volume: 7 Issue: 5 Pages: 189-195
  • DOI: 10.5582/ddt.2013.v7.5.189
  • NAID: 130005054779
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed
• [Journal Article] Evaluation of antiviral activity of Oligonol, an extract of <i>Litchi chinensis</i>, against betanodavirus (2013)
  • Author(s): Ichinose T, Musyoka TM, Watanabe K, Kobayashi N
  • Journal Title: Drug Discoveries & Therapeutics Volume: 7 Issue: 6 Pages: 254-260
  • DOI: 10.5582/ddt.2013.v7.6.254
  • NAID: 130003397391
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed
• [Presentation] 新たな作用機序をもつ抗インフルエンザウイルス薬の開発 (2017)
  • Author(s): 渡辺（渡邊）健、石川岳志、水田賢志、中垣岳大、大滝大樹、濱田剛、田中義正、西田教行
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2017-03-27
• [Presentation] Drug discovery for influenza virus using in silico and in vitro approaches (2016)
  • Author(s): 3.Juliann Nzembi Makau, Ken Watanabe, Takeshi Ishikawa, Satoshi Mizuta, Tsuyoshi Hamada, Nobuyuki Kobayashi, Noriyuki Nishida
  • Organizer: The 15th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2016-09-08
  • Int'l Joint Research
• [Presentation] Discovery of influenza virus inhibitors using in silico and in vitro approaches (2016)
  • Author(s): 2.Juliann Nzembi Makau, Ken Watanabe, Takeshi Ishikawa, Tsuyoshi Hamada, Nobuyuki Kobayashi, Noriyuki Nishida
  • Organizer: 第5回感染症若手フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2016-09-04
• [Presentation] Drug discovery for influenza virus (2016)
  • Author(s): 4.Juliann Nzembi Makau, Ken Watanabe, Takeshi Ishikawa, Tsuyoshi Hamada, Nobuyuki Kobayashi, Noriyuki Nishida
  • Organizer: The 3rd International Symposium for the Promotion of Science and Technology Innovation Cooperation between Africa and Japan
  • Place of Presentation: JICA市ヶ谷ビル（東京都新宿区）
  • Year and Date: 2016-07-13
  • Int'l Joint Research
• [Presentation] In silicoスクリーニングによる抗インフルエンザ化合物の探索 (2016)
  • Author(s): 渡辺健、石川岳志、水田賢志、大滝大樹、濱田剛、田中義正、西田教行
  • Organizer: 第136回日本薬学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-03-29
• [Presentation] インフルエンザウィルス感染に対するMethylglycoxalとNeuraminidase阻害薬の併用による相乗的阻害効果についての検討
  • Author(s): チャリャシリワン シリワン、松永 彩香、渡辺 健、小林 信之
  • Organizer: 第49回日本ウイルス学会九州支部総会
  • Place of Presentation: 長崎大学良順会館（長崎県長崎市）
• [Presentation] トルコ民族薬用植物の抗インフルエンザ活性: Alchemilla mollis 抽出物のインフルエンザウイルスに対する殺ウイルス活性の可能性
  • Author(s): Juliann Nzembi Makau、渡辺健、小林信之
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール（長崎県長崎市）
• [Patent(Industrial Property Rights)] キノリノン化合物および抗RNAウイルス治療薬 (2017)
  • Inventor(s): 水田賢志、渡邊健、西田教行、濱田剛、石川岳志、田中義正、大滝大樹
  • Industrial Property Rights Holder: 長崎大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-072230
  • Filing Date: 2017-03-31