Glycosylation of functional natural compounds for improving intestinal absorption
| Project/Area Number |
25460127
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | The Makino Memorial Foundation of Kochi Prefecture (2014-2015)
Nagoya City University (2013) |
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Principal Investigator |
Mizukami Hajime 公益財団法人高知県牧野記念財団, その他部局等, その他 (30128219)
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| Co-Investigator(Kenkyū-buntansha) |
TERASAKA Kazuyosi 名古屋市立大学, 大学院薬学研究科, 講師 (60405214)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 生理活性天然物 / 糖鎖構築 / 糖転移酵素 / 消化管吸収 / 植物機能性化合物 / 酵素的配糖体合成 / 消化管グルコシダーゼ / 機能性配糖体 / 酵素合成 |
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| Outline of Final Research Achievements |
We have isolate and characterized O- and C-glucosyltransferases from various plants including Gardenia jasminoides, Fragaria ×ananassa and Glycine max. By usig the recombinant glycosyltransferases so far obtained, a wide array of natural glycosides can be easily prepared.
To analyze the relationship between sugar chain structures of natural glycosides and intestinal absorption of these compounds, we have tried to prepare recombinant lactose-phlorizin hydrolase (LPH) originated from human or rat intestine.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] A 7-deoxyloganetic acid glucosyltransferase contributes a key step in secologanin biosynthesis in Madagascar periwinkle.2013
Author(s)
Asada, K., Salim, V., Masada-Atsumi, S., Edmunds, E., Nagatoshi, M., Terasaka, K., Mizukami, H., De-Luca, V.
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Journal Title
The Plant Cells
Volume: 25
Issue: 10
Pages: 4123-4134
DOI
Related Report
Peer Reviewed
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[Journal Article] Anti-allergic effects of enzymatically modified isoquercitrin (α-oligoglucosyl quercetin 3-O-glucoside), quercetin 3-O-glucoside, α-oligoglucosyl rutin, and quercetin, when administered orally to mice.2013
Author(s)
Makino, T., Kanemaru, M., Okuyama, S., Shimizu, R., Tanaka, H., Mizukami, H
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Journal Title
J.Nat. Med.
Volume: (in press)
Issue: 4
Pages: 881-886
DOI
Related Report
Peer Reviewed
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