| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Anethole expresses synergistic antifungal activity via restriction of over-expression of drug exhaust pump PDR5 and its transcription factor PDR3. We found that the restriction was involved in the restriction of PDR5 and PDR3 over-expression induced by LGE1, which is over-expressed as a result of deprivation of mitochondrial DNA. In addition, the synergy disappeared in strains lacking PMR1 and genes related to chromatin remodeling complexes SWI/SNF. Whereas anethole accelerated Ca2+ accumulation, drug exhaust was constitutively restricted in the strain described above. Therefore, these results indicated that regulatory mechanism of Ca2+ homeostasis was involved in regulation of genes related to drug exhaust and elevation of Ca2+ levels was provably secondary effects on the gene regulation.
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