The search and functional analysis of the natural compound promoting the differentiation of regulatory T cells.

• Project/Area Number: 25460132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Musashino University
• Principal Investigator: KOTANI Hitoshi 武蔵野大学, 薬学研究所, 助教 (10594640)
• Research Collaborator: INOUE Makoto 愛知学院大学, 薬学部, 教授 (50191888) ; YOSHIMURA Akihiko 慶應義塾大学, 医学部, 教授 (90182815) ; TANABE Hiroki 北陸大学, 薬学部, 准教授 (10415606) ; KATAKAWA Kazuaki 武蔵野大学, 薬学部, 講師 (90433606)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 制御性T細胞 / 免疫賦活 / 生薬 / 天然物 / 沈香 / 癌 / インターフェロンガンマ / 自己免疫疾患 / ジンコウ / サイトカイン / Treg

Outline of Final Research Achievements

In this study, I searched for the natural compound which regulated the differentiation of the regulatory T cell which controlled excessive immunity. As a result, I found that the ingredient included in the crude drug called JINKO inhibited the differentiation of the regulatory T cells from Naive T cells. This natural compound can activate immunity by restraining the differentiation of the regulatory T cells, and anti-tumor activity is expected.

Research Products

• [Journal Article] Rexinoids isolated from Sophora tonkinensis with a gene expression profile distinct from the synthetic rexinoid bexarotene. (2014)
  • Author(s): Inoue M, Tanabe H, Nakashima K, Ishida Y, Kotani H.
  • Journal Title: J Nat Prod. Volume: 25;77(7) Issue: 7 Pages: 1670-7
  • DOI: 10.1021/np5002016
  • Peer Reviewed
• [Journal Article] Retinoic acid receptor agonist activity of naturally occurring diterpenes. (2014)
  • Author(s): Hiroki Tanabe, Tomohisa Yasui, Hitoshi Kotani, Akito Nagatsu, Makoto Makishima, Sakae Amagaya, Makoto Inoue
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 22 Issue: 12 Pages: 3204-3212
  • DOI: 10.1016/j.bmc.2014.03.047
  • Peer Reviewed
• [Journal Article] CDK inhibitors suppress Th17 and promote iTreg differentiation, and ameliorate experimental autoimmune encephalomyelitis in mice. (2013)
  • Author(s): Yoshida H, Kotani H, Kondo T, Tani I, Wei X, Tsuruta S, Kimura A, Asakawa M, Ito M, Nagai S, Yoshimura A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 435 Issue: 3 Pages: 378-384
  • DOI: 10.1016/j.bbrc.2013.04.096
  • Peer Reviewed
• [Journal Article] TRAF6 Is Essential for Maintenance of Regulatory T Cells That Suppress Th2 Type Autoimmunity. (2013)
  • Author(s): Muto G, Kotani H, Kondo T, Morita R, Tsuruta S, Kobayashi, T., Luche H, Fehling HJ, Walsh M, Choi Y, Yoshimura A.
  • Journal Title: PLoS One Volume: 8 (9) Issue: 9 Pages: e74639-e74639
  • DOI: 10.1371/journal.pone.0074639
  • Peer Reviewed
• [Presentation] 制御性T細胞の分化誘導を調節する生薬エキスのスクリーニング (2014)
  • Author(s): 小谷仁司 中川竜介 吉村昭彦 渡辺恵史
  • Organizer: 日本生薬学会第61回年会
  • Place of Presentation: 福岡大学薬学部
  • Year and Date: 2014-09-13 – 2014-09-14