| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
A library consisting of 200 kinds of methanolic extracts from medicinal plants, was screened cancer stem cell inhibitory activity. Extracts of Saururus chinensis, Trichosanthes kirilowii and Euphorbia fischeriana were bioactive. The methanol extract of S. chinensis was fractionated by a Bioassay Guided Fractionation method to give 10 kinds of tetrahydrofuran lignan derivatives, including two novel compounds, as well as three kinds of 8, 4'-oxyneolignan derivatives and three other lignan derivatives. Two tetrahydrofuran-type lignans with characteristic poly-oxygenated functional groups on the aromatic ring, namely, saurucinol I and rel-(7R, 8S, 7'S, 8'S)-4',5'-methylenedioxy- 3,4,5,3'-tetramethoxy-7,7'-epoxylignan, showed the most potent activity. Saurucinol I showed the inhibitory effect of mammosphere formation, and inhibited the CD44+/24- expression level which is an indicator of human breast cancer stem cells.
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