| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Eleven triterpene synthase homologs are found in Glycine max genome. Among them, one homolog (locus name: Glyma.20G192700) is not transcribed in G. max. This gene was amplified by PCR with genome DNA as a template, from which introns were removed. Resultant DNA was expressed in Saccharomyces cerevisiae. Accumulated product in yeast cells was analyzed by GC-MS, and identified as lupeol which is known compound. Although this result is deviated substantially from original purpose to produce new triterpene, it is succeeded in revealing that lupeol had occurred, or will occur in G. max, by functional analysis of the genomic gene.
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