Production of new triterpene and establishment of high production system of triterpene
Project/Area Number |
25460142
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Natural medicines
|
Research Institution | Nigata University of Phermacy and Applied Life Sciences |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | トリテルペン / トリテルペン合成酵素 / ゲノム遺伝子 / シロイヌナズナ / ダイズ / ルペオール / 生合成 / ゲノム |
Outline of Final Research Achievements |
Eleven triterpene synthase homologs are found in Glycine max genome. Among them, one homolog (locus name: Glyma.20G192700) is not transcribed in G. max. This gene was amplified by PCR with genome DNA as a template, from which introns were removed. Resultant DNA was expressed in Saccharomyces cerevisiae. Accumulated product in yeast cells was analyzed by GC-MS, and identified as lupeol which is known compound. Although this result is deviated substantially from original purpose to produce new triterpene, it is succeeded in revealing that lupeol had occurred, or will occur in G. max, by functional analysis of the genomic gene.
|
Report
(4 results)
Research Products
(3 results)
-
[Journal Article] Recombinant yeast as a functional tool for understanding bitterness and cucurbitacin biosynthesis in watermelon(Citrullus spp.)2015
Author(s)
91)Rachel Davidovich-Rikanati, Lior Shalev, Nadine Baranes, Ayala Meir, Maxim Itkin, Shahar Cohen, Kobi Zimbler, Vitaly Portnoy, Yutaka Ebizuka, Masaaki Shibuya, Yosef Burger, Nurit Katzir, Arthur A. Schaffer, Efraim Lewinsohn1and Ya’akov Tadmor
-
Journal Title
Yeast
Volume: 32
Pages: 103-114
DOI
Related Report
Peer Reviewed / Open Access
-
-