| Project/Area Number |
25460145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Natural medicines
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
Noji Masaaki 徳島文理大学, 薬学部, 准教授 (80271534)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ミシマサイコ / サイコサポニン生合成 / シトクロムP450 / グリコシルトランスフェラーゼ |
|---|
| Outline of Final Research Achievements |
In order to identify the genes involved in saikosaponin biosynthesis, we first established expressed sequence tag (EST) databases of roots, leaves, and stems from Bupleurum falcatum L. by using next-generation sequencing platforms.
Some candidate genes that may be concerned with the biosynthesis of saikosaponins were available by searching EST databases of B. falcatum. Sixteen new cDNAs encoding cytochrome P450 were isolated and were characterized using a yeast expression system. One of these cytochrome P450s, R144792, catalyzes the C-28 hydroxylation of beta-amyrin, and another cytochrome P450, Rnn2525, catalyzes the C-16 alpha hydroxylation of beta-amyrin, that are essential for the biosynthesis of saikosaponins. Furthermore, two new cDNAs encoding glycosyltransferase were isolated from B. falcatum.
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