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Elucidation of machinery for saikosaponin biosynthesis in Bupleurum falcatum L.

Research Project

Project/Area Number 25460145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Natural medicines
Research InstitutionTokushima Bunri University

Principal Investigator

Noji Masaaki  徳島文理大学, 薬学部, 准教授 (80271534)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミシマサイコ / サイコサポニン生合成 / シトクロムP450 / グリコシルトランスフェラーゼ
Outline of Final Research Achievements

In order to identify the genes involved in saikosaponin biosynthesis, we first established expressed sequence tag (EST) databases of roots, leaves, and stems from Bupleurum falcatum L. by using next-generation sequencing platforms.
Some candidate genes that may be concerned with the biosynthesis of saikosaponins were available by searching EST databases of B. falcatum. Sixteen new cDNAs encoding cytochrome P450 were isolated and were characterized using a yeast expression system. One of these cytochrome P450s, R144792, catalyzes the C-28 hydroxylation of beta-amyrin, and another cytochrome P450, Rnn2525, catalyzes the C-16 alpha hydroxylation of beta-amyrin, that are essential for the biosynthesis of saikosaponins. Furthermore, two new cDNAs encoding glycosyltransferase were isolated from B. falcatum.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2016 2015 2014

All Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] ミシマサイコ由来のシトクロムP450遺伝子の単離と解析2016

    • Author(s)
      山口誠美、野路征昭、遠藤加奈子、兼目裕充、高橋宏暢、岡田岳人、浅川義範、梅山明美、豊田正夫
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] サイコサポニン生合成に関与する糖転移酵素遺伝子の探索2016

    • Author(s)
      新見明子、野路征昭、西森千晶、兼目裕充、高橋宏暢、岡田岳人、浅川義範、梅山明美、豊田正夫
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular cloning and characterization of structural genes for saikosaponin biosynthetic enzymes in Bupleurum falcatum L.2015

    • Author(s)
      Masaaki Noji, Hiromichi Kenmoku, Taketo Okada, Hironobu Takahashi, Akemi Umeyama, Masao Toyota, Yoshinori Asakawa
    • Organizer
      Inaugural Symposium of the Phytochemical Society of Asia 2015
    • Place of Presentation
      Tokushima, Japan
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] サイコサポニン生合成に関与するシトクロムP450遺伝子の単離と機能解析2015

    • Author(s)
      野路征昭、中渡瀬光華、武岡志保、後藤真弓、兼目裕充、高橋宏暢、岡田岳人、豊田正夫、浅川義範
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] サイコサポニン生合成に関与するシトクロムP450の解析2014

    • Author(s)
      野路征昭、後藤真弓、武岡志保、兼目裕充、高橋宏暢、岡田岳人、豊田正夫、浅川義範
    • Organizer
      日本生薬学会第61回年回
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-09-13 – 2014-09-14
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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