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Analysis of cytotoxicity to host cells of live bacilli among Mycobacterium species

Research Project

Project/Area Number 25460170
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionNagoya City University

Principal Investigator

Takii Takemasa  名古屋市立大学, 薬学研究科(研究院), 准教授 (80244573)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords感染症 / 結核菌 / 細胞傷害性 / サイトカイン / 抗酸化活性 / 細胞傷害活性 / インターロイキン / 腫瘍壊死因子 / 病原性 / 核酸 / ブースターワクチン / インターロイキン1 / 老化
Outline of Final Research Achievements

We have previously reported that virulent Mycobacteria exhibit strong cytotoxicity to host cells (JICR, 2001). Supported by this research fund, we revealed that the anti-oxidative activity of those bacilli would relate to the long time survival in the host cells and the strong activity of cytokines production from infected host cells (Kekkaku, 2015, Microbiol Immunol., 2015). So, we expected that the anti-oxidative activity of the bacilli would relate to the cytotoxicity to the host cells.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Sensors for redox stress in Mycobacteria2016

    • Author(s)
      Takemasa Takii
    • Journal Title

      kekkaku

      Volume: 90(6)

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Early-shared Mycobacterium bovis bacillus Calmette-Guérin sub-strains induce Th1 cytokine production in vivo.2015

    • Author(s)
      Taniguchi K, Miyatake Y, Hayashi D, Takami A, Itoh S, Yamamoto S, Hida S, Onozaki K, Takii T.
    • Journal Title

      Microbiol Immunol.

      Volume: 59 Issue: 11 Pages: 684-689

    • DOI

      10.1111/1348-0421.12326

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] SENSORS IN MYCOBACTERIA FOR THE DETECTION OF REDOX STRESS2015

    • Author(s)
      Takii T.
    • Journal Title

      Kekkaku.

      Volume: 90 Pages: 579-591

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reactivation of immune responses against Mycobacterium tuberculosis by boosting with the CpG oligomer in aged mice primarily vaccinated with Mycobacterium bovis BCG2013

    • Author(s)
      Taniguchi K, Takii T, Yamamoto S, Maeyama J, Iho S, Maruyama M, Iizuka N, Ozeki Y, Matsumoto S, Hasegawa T, Miyatake Y, Itoh S, Onozaki K.
    • Journal Title

      Immun Ageing

      Volume: 10 Issue: 1 Pages: 25-25

    • DOI

      10.1186/1742-4933-10-25

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood coagulation by binding to prothrombin and factor Xa via theirγ-carboxyglutamic acid (Gla) domain2013

    • Author(s)
      Itoh S, Yokoyama R, Kamoshida G, Fujiwara T, Okada H, Takii T, Tsuji T, Fujii S, Hashizume H, Onozaki K
    • Journal Title

      J Biol Chem

      Volume: 288 Issue: 30 Pages: 21569-21580

    • DOI

      10.1074/jbc.m113.451419

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bacterial sphingophospholipids containing non-hydroxy fatty acid activate murine macrophages via Toll-like receptor 4 and stimulate bacterial clearance2013

    • Author(s)
      Fujiwara, N.
    • Journal Title

      Biochim. Biophys. Acta.

      Volume: Mar 29 Issue: 6 Pages: 1177-84

    • DOI

      10.1016/j.bbalip.2013.03.008

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 結核ワクチンBCG Tokyo172 サブタイプ間のレドックス関連遺伝子の発現誘導の解析2016

    • Author(s)
      小川翔大、高見篤郎、富田陽香、徳田美季、伊藤佐生智、林大介、山本三郎、肥田重明、小野嵜菊夫、瀧井猛将
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mycobacterium avium の酸性環境下で誘導されるアルギニンデイミナーゼの解析2016

    • Author(s)
      瀧井猛将、小川翔大、山本龍二、伊藤佐生智、堀田康弘、大原直也、小川賢二、八木哲也、前田伸司、藤原永年、山崎利雄、西森 敬、後藤義孝、肥田重明、小野嵜菊夫
    • Organizer
      第89回日本細菌学会
    • Place of Presentation
      大阪国際交流会館
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] A Contribution of arginine deiminase pathway to the acid tolerance response in Mycobacterium avium sub-strains avium and hominissuis2016

    • Author(s)
      Takemasa Takii, Tetsuya Yagi, Kenji Ogawa, Shinji Maeda, Kei Nishimori, Nagatoshi Fujiwara, Yoshitaka Goto, Toshio Yamazaki, Naoya Ohara, and Kikuo Onozaki
    • Organizer
      The U.S.–Japan Cooperative Medical Sciences Program presents 50th Anniversary Celebration 18th International Conference on Emerging Infectious Diseases (EID)
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2016-01-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トリ型結核菌Mycobacterium aviumにおける酸耐性機構の解析2015

    • Author(s)
      富田陽香、小川翔大、花村菜月、宮田江里香、伊藤佐生智、肥田重明、小野嵜菊夫、瀧井猛将
    • Organizer
      日本病院薬剤師会東海ブロック日本薬学会東海支部合同学術大会2015
    • Place of Presentation
      金城学院大学
    • Year and Date
      2015-11-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Contribution of arginine deiminase pathway to the acid tolerance response in Mycobacterium avium substrains2015

    • Author(s)
      Takemasa Takii
    • Organizer
      NCU 65th Anniversary Event Biodiversity and environmental medicine in South East and East Asia base on molecular biology 2015.
    • Place of Presentation
      Nagoya City Univercity
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] BCGワクチンの副反応誘発株における遺伝子変異と宿主細胞に対する作用の解析2015

    • Author(s)
      瀧井猛将,吉田志緒美,有川健太郎,藤山理世,岩本朋忠
    • Organizer
      第90回日本結核病学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 新たな抗結核薬の探索2015

    • Author(s)
      瀧井猛将
    • Organizer
      第90回日本結核病学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] BCG Tokyo172 typeⅠ,typeⅡ間でのマクロファージからのサイトカイン誘導能の検討2015

    • Author(s)
      高見篤郎,林 大介,山本三郎,瀧井猛将
    • Organizer
      第85回実験結核研究会
    • Place of Presentation
      長崎
    • Year and Date
      2015-03-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Mycobacterium bovis BCGの酸化ストレスによるredox関連遺伝子発現変化の解析2014

    • Author(s)
      高見篤郎,富田陽香,前山順一,山本三郎,瀧井猛将
    • Organizer
      フォーラム2014:衛生薬学・環境トキシコロジー
    • Place of Presentation
      筑波
    • Year and Date
      2014-09-02
    • Related Report
      2014 Research-status Report
  • [Presentation] Mycobacterium bovis BCGの過酸化水素ストレスによるredox関連遺伝子発現変化の解析2014

    • Author(s)
      高見篤郎,富田陽香,前山順一,山本三郎,瀧井猛将
    • Organizer
      第60回日本薬学会東海支部総会・大会2014
    • Place of Presentation
      鈴鹿
    • Year and Date
      2014-07-05
    • Related Report
      2014 Research-status Report
  • [Presentation] BCG Tokyo 172 Type I , Type II間の酸化ストレス感受性とマクロファージ内生存能及び サイトカイン誘導能の比較研究2014

    • Author(s)
      瀧井猛将,伊藤佐生智,大原直也,前山順一,林 大介,山本三郎
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Presentation] マクロファージ感染Mycobacterim aviumに対するクラリスロマイシンとバフィロマイシンの相乗効果2014

    • Author(s)
      伊藤佐生智,小川賢二,八木哲也,瀧井猛将
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 抗酸菌とレドックス2014

    • Author(s)
      瀧井猛将
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] BCG Tokyo172-1に存在するサブポピュレーションの比率変化に関する検討2014

    • Author(s)
      大原直也,趙娜,和田崇之,藤原永年,前田伸司,山本三郎,瀧井猛将,前山順一
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] BCGワクチン接種による副反応事例から得たBCG Tokyo172株の分子特性2014

    • Author(s)
      岩本朋忠,有川健太郎,藤山理世,松林恵介,水尻節子,白井千香,伊地智昭浩,瀧井猛将
    • Organizer
      第89回日本結核病学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] BCG Tokyo172 type I, type II間でのマクロファージからのサイトカイン誘導能の検討2014

    • Author(s)
      瀧井猛将,高見篤郎,林 大介,山本三郎
    • Organizer
      第84回実験結核研究会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-05-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Boosting effect of Oligo-B in super aged mice primarily vaccinated with Mycobacterium bovis BCG on the protection activity against Mycobacterium tuberculosis infection

    • Author(s)
      Takemasa Takii, Keiichi Taniguchi, Saburo Yamamoto, Jun-ichi Maeyama, Sumiko Iho, Mitsuo Maruyama, Narushi Iizuka, Yuriko Ozeki, Sohkichi Matsumoto, Saotomo Itoh and Kikuo Onozaki
    • Organizer
      Front Range Mycobacteria Conference
    • Place of Presentation
      Fort Collins, CO, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Staphylococcal superantigen-like 3はToll-like receptor 2に結合する

    • Author(s)
      横山領介、伊藤 佐生智、 奥村拓也、鴨志田 剛、瀧井猛将、藤井聡、辻勉、小野嵜 菊夫、藤原俊伸
    • Organizer
      第14回PharmacoHematologyシンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 非結核性抗酸菌Mycobacterium aviumのpH依存的なarcAの発現調節に関する研究

    • Author(s)
      小川翔大、花村菜月、宮田江里香、藤原俊伸、伊藤佐生智、瀧井猛将
    • Organizer
      第25回微生物シンポジウム
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] マクロファージ内Mycobacterium aviumのClarithromycin感受性に及ぼす Bafilomycin A1の効果

    • Author(s)
      筑比地慧、宮田江里香、花村菜月、小川翔大、藤原俊伸、伊藤佐生智、瀧井猛将
    • Organizer
      第25回微生物シンポジウム
    • Place of Presentation
      静岡
    • Related Report
      2013 Research-status Report
  • [Presentation] 菌体内pHの低下によるMycobacterium aviumのarcA mRNAの発現変化の解析

    • Author(s)
      小川翔大、小川賢二、八木哲也、大原直也、後藤義孝、藤原永年、前田伸司、伊藤佐生智、瀧井猛将
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] BCG Tokyo-172 Type I , Type II間の酸化ストレス感受性とマクロファージ内生存能の比較研究

    • Author(s)
      瀧井猛将、宮竹佑治、小川翔大、谷口恵一、富田陽香、徳田美季、伊藤佐生智、大原直也、前山順一、林 大介、山本三郎
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
  • [Presentation] タバコ主流煙に含まれるマウスコラーゲン誘導関節炎増悪物質の探索

    • Author(s)
      竹野聖史、秋田邦彦、足達実季、伊藤佐生智、山中淳平、北川慎也、早川和一、小野崎菊夫、瀧井猛将
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] Mycobacterium aviumにおける酸性環境下でのアルギニンデイミナーゼの発現誘導の解析

    • Author(s)
      小川翔大、小川賢二、八木哲也、大原直也、後藤義孝、藤原永年、前田伸司、伊藤佐生智、筑比地慧、宮田江里香、高見篤郎、徳田美季、富田陽香、小野崎菊夫、瀧井猛将
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] 結核ワクチンBCG Tokyo-172 タイプ I , タイプ II間の酸化ストレス感受性とマクロファージ内生存能及びサイトカイン誘導能の比較研究

    • Author(s)
      瀧井猛将、小川翔大、谷口恵一、宮竹祐治、富田陽香、徳田実季、伊藤佐生智、大原直也、前山順一、林大介、山本三郎
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Remarks]

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/esk/gyoseki.html

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] ワクチンアジュバント及び免疫増強剤2016

    • Inventor(s)
      瀧井猛将,小野嵜菊夫,山中淳平,北川慎也,竹野聖史
    • Industrial Property Rights Holder
      瀧井猛将,小野嵜菊夫,山中淳平,北川慎也,竹野聖史
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-01-06
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] ワクチンアジュバント及び免疫増強剤2014

    • Inventor(s)
      瀧井猛将,小野嵜菊夫,伊藤佐生智,山中淳平,竹野聖史,北川慎也
    • Industrial Property Rights Holder
      名古屋市立大学、名古屋工業大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-140033
    • Filing Date
      2014-07-07
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 抗結核薬のスクリーニング方法2013

    • Inventor(s)
      瀧井猛将、前田伸司、和田崇之、伊藤佐生智、小野嵜菊夫、長谷川倫宏
    • Industrial Property Rights Holder
      中部TLO
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-05-15
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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