Application of Staphylococcual immune disturbing toxins, SSLs to development of newly drugs and treatments.

• Project/Area Number: 25460171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Itoh Saotomo 名古屋市立大学, 薬学研究科(研究院), 講師 (70308013)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細菌毒素 / 免疫回避 / 黄色ブドウ球菌毒素 / スーパー抗原様毒素

Outline of Final Research Achievements

Staphylococcal superantigen-like proteins(SSL) recognize and disturb various host immune molecules, and involved in immune evasion of the bacteria. In this study, we analayzed molecular mechanisms of binding SSLs to host target molecules.

Research Products

• [Journal Article] Early-shared Mycobacterium bovis bacillus Calmette-Guérin sub-strains induce Th1 cytokine production in vivo. (2015)
  • Author(s): Taniguchi K, Miyatake Y, Hayashi D, Takami A, Itoh S, Yamamoto S, Hida S, Onozaki K, Takii T.
  • Journal Title: Microbiol Immunol. Volume: 59 Issue: 11 Pages: 684-689
  • DOI: 10.1111/1348-0421.12326
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of liposomal nanoconstructs targeting P-selectin (CD62P)-expressing cells by using a sulfated derivative of sialic acid (2014)
  • Author(s): Itoh S, Kawano K, Takeshita K, Maitani Y, Tsuji T
  • Journal Title: Pharm Res Volume: (in press) Issue: 10 Pages: 2868-2875
  • DOI: 10.1007/s11095-014-1383-6
  • Peer Reviewed
• [Journal Article] Reactivation of immune responses against Mycobacterium tuberculosis by boosting with the CpG oligomer in aged mice primarily vaccinated with Mycobacterium bovis BCG (2013)
  • Author(s): Taniguchi K, Takii T, Yamamoto S, Maeyama J, Iho S, Maruyama M, Iizuka N, Ozeki Y, Matsumoto S, Hasegawa T, Miyatake Y, Itoh S, Onozaki K.
  • Journal Title: Immun Ageing Volume: 10 Issue: 1 Pages: 25-25
  • DOI: 10.1186/1742-4933-10-25
  • Peer Reviewed
• [Journal Article] Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood coagulation by binding to prothrombin and factor Xa via theirγ-carboxyglutamic acid (Gla) domain (2013)
  • Author(s): Itoh S, Yokoyama R, Kamoshida G, Fujiwara T, Okada H, Takii T, Tsuji T, Fujii S, Hashizume H, Onozaki K
  • Journal Title: J Biol Chem Volume: 288 Issue: 30 Pages: 21569-21580
  • DOI: 10.1074/jbc.m113.451419
  • Peer Reviewed
• [Journal Article] Cigarette smoke condensate extracts induce IL-1 beta production from rheumatoid arthritis patient-derived synoviocytes, but not osteoarthritis patient-derived synoviocytes, through aryl hydrocarbon receptor-dependent NF-kappa B activation and novel NF-kappa B sites. (2013)
  • Author(s): Adachi M, Okamoto S, Chujyo S, Arakawa T, Yokoyama M, Yamada K, Hayashi A, Akita K, Takeno M, Ito S, Takii T, Waguri-Nagaya Y, Otsuka T, Hayakawa K, Miyazawa K, Onozaki K.
  • Journal Title: J Interferon & Cytokine Research Volume: 33 Issue: 6 Pages: 297-307
  • DOI: 10.1089/jir.2012.0107
  • Peer Reviewed
• [Presentation] 結核ワクチンBCG Tokyo172 サブタイプ間のレドックス関連遺伝子の発現誘導の解析 (2016)
  • Author(s): 小川翔大、高見篤郎、富田陽香、徳田美季、伊藤佐生智、林大介、山本三郎、肥田重明、小野嵜菊夫、瀧井猛将
  • Organizer: 日本薬学会 第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] 黄色ブドウ球菌スーパー抗原様タンパク質SSL10の血液凝固抑制の作用機序の解析及びその責任領域の同定 (2016)
  • Author(s): 伊藤 佐生智，村瀬 千鶴子，瀧井 猛将，小野嵜 菊夫，肥田 重明
  • Organizer: 日本薬学会 第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] トリ型結核菌Mycobacterium aviumにおける酸耐性機構の解析 (2015)
  • Author(s): 富田陽香、小川翔大、山本龍二、花村菜月、宮田江里香、堀田康弘、伊藤佐生智、肥田重明、瀧井猛将
  • Organizer: 日本薬学会東海支部会合同学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-11-01
• [Presentation] 酸性環境下でのMycobacterium avium のアミノ酸代謝とアンモニア産生との関係 (2015)
  • Author(s): 富田陽香、小川翔大、花村菜月、宮田江里香、伊藤佐生智、肥田重明、小野嵜菊夫、瀧井猛将
  • Organizer: 第27回微生物シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-09-04
• [Presentation] 抗結核作用を有するジチオカルバメート糖誘導体（OCT313）の作用機序の解析 (2015)
  • Author(s): 徳田美季、長谷川倫宏、寺本真由美、伊藤佐生智、肥田重明、小野嵜菊夫、瀧井猛将
  • Organizer: 第27回微生物シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-09-04
• [Presentation] 黄色ブドウ球菌スーパー抗原様タンパク質SSL10の血液凝固抑制メカニズムの解析 (2015)
  • Author(s): 伊藤佐生智，村瀬千鶴子，瀧井猛将，小野嵜菊夫，肥田重明
  • Organizer: 第27回微生物シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-09-04
• [Presentation] Staphylococcal superantigen-like protein 5 と Matrix metalloproteinase 9 の結合 (2015)
  • Author(s): 栗坂知里、奥 輝明、伊藤佐生智、辻 勉
  • Organizer: 第16回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2015-06-13
• [Presentation] 黄色ブドウ球菌毒素を認識するRNAアプタマーの創製 (2015)
  • Author(s): 伊藤 佐生智
  • Organizer: 第9回長野ミーティング「生物資源の有効活用を目指して」
  • Place of Presentation: 長野
  • Year and Date: 2015-01-25 – 2015-01-26
• [Presentation] 黄色ブドウ球菌毒素SSLの標的分子の探索 (2014)
  • Author(s): 伊藤 佐生智
  • Organizer: 第15回 Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2014-05-23 – 2014-05-24
  • Invited
• [Presentation] BCG Tokyo 172 Type I , Type II間の酸化ストレス感受性とマクロファージ内生存能及びサイトカイン誘導能の比較研究 (2014)
  • Author(s): 瀧井猛将、伊藤佐生智、大原直也、前山順一、林 大介、山本三郎
  • Organizer: 第89回日本結核病学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-09 – 2014-05-10
• [Presentation] マクロファージ感染Mycobacterim aviumに対するクラリスロマイシンとバフィロマイシンの相乗効果 (2014)
  • Author(s): 伊藤佐生智、小川賢二、八木哲也、瀧井猛将
  • Organizer: 第89回日本結核病学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-09 – 2014-05-10
• [Presentation] Identification of host target proteins of staphylococcal superantigenlike exotoxins. (2014)
  • Author(s): 伊藤 佐生智
  • Organizer: 第８回「長野ミーティング：生物資源の有効利用を目指して」
  • Place of Presentation: 長野
• [Presentation] Staphylococcal superantigen-like protein 10 (SSL10) binds to vitamin K-dependent blood coagulation factors via their γ-carboxyglutamic acid (Gla) domain and inhibits blood coagulation. (2013)
  • Author(s): Saotomo Itoh, Ryosuke Yokoyama, Go Kamoshida, Toshinobu Fujiwara, Hiromi Okada, Takemasa Takii, Tsutomu Tsuji, Satoshi Fujii, Hideki Hashizume, and Kikuo Onozaki
  • Organizer: EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology
  • Place of Presentation: Heidelberg, Germany
• [Presentation] Staphylococcal superantigen like 3(SSL3)はtoll like receptor 2（TLR2）の細胞外ドメインに結合し，TLR2リガンドによるマクロ ファージのサイトカイン産生を抑制する (2013)
  • Author(s): 横山領介，伊藤佐生智，鴨志田剛，奥村拓也，藤原俊伸，瀧井猛将，藤井聡，辻勉，小野嵜菊夫
  • Organizer: 第58回日本ブドウ球菌研究会
  • Place of Presentation: 東京
• [Presentation] Staphylococcal superantigen-like protein 5 (SSL5)とその宿主側標的分子matrixmetalloproteinase (MMP)-9の結合様式の解析 (2013)
  • Author(s): 花井 朱璃，伊藤 佐生智，藤原 俊伸，瀧井 猛将，辻 勉，小野嵜 菊夫
  • Organizer: 第２５回微生物シンポジウム
  • Place of Presentation: 静岡
• [Presentation] Staphylococcal superantigen-like 3はToll-like receptor 2に結合する (2013)
  • Author(s): 横山領介，伊藤 佐生智， 奥村拓也，鴨志田 剛，瀧井猛将，藤井聡，辻勉，小野嵜 菊夫，藤原俊伸
  • Organizer: 第14回PharmacoHematologyシンポジウム
  • Place of Presentation: 東京