• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism and regulation of immune activation cascade by aminopeptidase contained exosome.

Research Project

Project/Area Number 25460172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionTeikyo Heisei University

Principal Investigator

OGAWA Yuko  帝京平成大学, 薬学部, 准教授 (30267330)

Co-Investigator(Renkei-kenkyūsha) TSUJIMOTO Masafumi  帝京平成大学, 薬学部, 教授 (00281668)
YANOSHITA Ryohei  帝京平成大学, 薬学部, 教授 (90267336)
GOTO Yoshikuni  帝京平成大学, 薬学部, 講師 (90455345)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsexosome / マクロファージ / DPP IV / 唾液 / LPS / Toll like receptor 4 / 一酸化窒素 / 細胞間情報伝達 / エキソソーム / インターフェロンーγ / ERAP1 / 貪食
Outline of Final Research Achievements

Human whole saliva contains two types of extracellular vesicles. One type highly expresses dipeptidyl peptidase-4 (DPP4) and the other type rarely expresses them. We designate former as DPP IV-exosome (DPP4exo), and later as deficient DPP IV-exosome (dDPP4exo). DPP4exo contained lipopolysaccharide (LPS) abundantly, and dDPP4exo contained it slightly. Although large amount of LPS interacted weakly with DPP4exo or existed separately forming micelle, part of LPS bind DPP4exo tightly. DPP4exo alone did not activate murine macrophage, however, DPP4exo with interferon-gamma produce high level of nitric oxide (NO) from murine macrophage. dDPP4exo did not cause NO production, even when interferon-gamma was added. DPP4exo is presumed to induce NO production from macrophage, when inflammation caused by bacterial infection, and associated with increased levels of interferon-gamma in oral cavity.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Products Report
  • Research Products

    (12 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) Remarks (4 results)

  • [Journal Article] Next-Generation Sequencing of Protein-Coding and Long Non-protein-Coding RNAs in Two Types of Exosomes Derived from Human Whole Saliva2016

    • Author(s)
      Yuko Ogawa, Masafumi Tsujimoto, Ryohei Yanoshita
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 39 Issue: 9 Pages: 1496-1507

    • DOI

      10.1248/bpb.b16-00297

    • NAID

      130005415106

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      Products Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of Phenylalanine 297 in the Construction of the Substrate Pocket of Human Aminopeptidase B.2015

    • Author(s)
      Ohnishi A., Watanabe J., Ogawa Y., Goto Y., Hattori A., Tsujimoto M.
    • Journal Title

      Biochemistry

      Volume: 54(39) Issue: 39 Pages: 6062-70

    • DOI

      10.1021/acs.biochem.5b00964

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of glutamine-169 in the substrate recognition of human aminopeptidase B2014

    • Author(s)
      Ogawa Y, Ohnishi A, Goto Y, Sakuma Y, Watanabe J, Hattori A, Tsujimoto M
    • Journal Title

      Biochim Biophys Acta

      Volume: 1840 Issue: 6 Pages: 1872-1881

    • DOI

      10.1016/j.bbagen.2014.01.002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト唾液由来エキソソームに存在するJPSの存在状態の解析2016

    • Author(s)
      小川 裕子, 粂田 奈宝子, 矢ノ下 良平
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター (宮城県仙台市)
    • Year and Date
      2016-09-26
    • Related Report
      Products Report
  • [Presentation] ヒト唾液由来エキソソームの消化管内環境における安定性の検討2015

    • Author(s)
      粂田 奈宝子
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒト唾液由来エキソソームの消化酵素に対する安定性の検討2015

    • Author(s)
      小川 裕子, 秋元 義弘, 粂田 奈宝子, 谷口 由真, 米川 綾, 小倉 葵, 矢ノ下 良平
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      デザイン・クリエイティブセンター神戸(兵庫県神戸市)
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト唾液エキソソームによるマクロファージの活性化2014

    • Author(s)
      小川(池本) 裕子, 粂田 奈宝子, 後藤 芳邦, 池本 守, 辻本 雅文, 矢ノ下 良平
    • Organizer
      第87回日本生化学会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト唾液由来エキソソームの安定性の検討

    • Author(s)
      小川裕子, 角田 圭, 登松 千晴, 秋元 義弘, 矢ノ下良平
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市総合体育館
    • Related Report
      2013 Research-status Report
  • [Remarks] 膜機能研究ユニット

    • URL

      http://pharm.thu.ac.jp/research/unit/makukinou.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 帝京平成大学 膜機能研究ユニット

    • URL

      http://pharm.thu.ac.jp/research/unit/makukinou.html

    • Related Report
      2014 Research-status Report
  • [Remarks] 帝京平成大学 薬学部 膜機能研究ユニットホームページ

    • URL

      http://pharm.thu.ac.jp/research/unit/makukinou.html

    • Related Report
      2013 Research-status Report
  • [Remarks]

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi