Mechanisms of prostanoids release from the cells
| Project/Area Number |
25460184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Tohoku University (2014-2015)
Hokkaido University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | プロスタノイド / トランスポーター / 細胞外放出 / エイコサノイド / ABCC4 / 薬物輸送担体 |
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| Outline of Final Research Achievements |
The purpose of this study is to estimate the contribution of transporters to the prostanoids release from the cells. We established the LC/MS/MS analytical methods and applied to the in vitro studies using determination of prostanoids released from culture cells and vesicullar transport assay. In the experimentes using MRP inhibitors and MRP4 siRNA, MRP4 partly contributes to the release of PGE2 and PGF2α but not TXB2 from A549 cells. EPA-derived prostanoids also showed similar results.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Regulation of the Expression and Activity of Glucose and Lactic Acid Metabolism-Related Genes by Protein Kinase C in Skeletal Muscle Cells2013
Author(s)
Otake S, Kobayashi M, Narumi K, Sasaki S, Kikutani Y, Furugen A, Watanabe M, Takahashi N, Ogura J, Yamaguchi H, Iseki K
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 36
Issue: 9
Pages: 1435-1439
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed
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