Development of a model for the monitoring ontogenic expression of CYP3A by used of secreted reporter-expressing iPS cells

• Project/Area Number: 25460195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: University of Shizuoka (2015-2016); Tohoku Pharmaceutical University (2013-2014)
• Principal Investigator: Sasaki Takamitsu 静岡県立大学, 薬学部, 講師 (20382674)
• Co-Investigator(Kenkyū-buntansha): 永田 清 東北医科薬科大学, 薬学部, 教授 (80189133)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: iPS細胞 / CYP3A4 / レポーター遺伝子 / 肝細胞 / 薬物代謝学 / CYP3A

Outline of Final Research Achievements

Human induced pluripotent stem cells (iPSCs) are a valuable source of hepatocytes for applications in drug development studies. Human CYP3A genes show unique expression changes, which are influenced by human development, and this developmental switch in expression between CYP3A4 and CYP3A7 genes occurs during the first 1 - 2 years after birth. In this study, based on the reproduction of the CYP3A expression pattern in the development, we aimed to establish methods for the differentiation of iPSCs into functional hepatocytes and evaluate novel transcriptional mechanism of pharmacokinetic-related genes. As results, we identified novel mechanisms involved in the induction of CYP3A4 and MRP3, and modified our previously established methods based on these results.

Research Products

• [Int'l Joint Research] NCI/NIH(米国)
• [Journal Article] Construction of the Database of Rat Repeated-dose Toxicity Tests of Pesticides for the Toxicological Characterization of Hepatocyte Hypertrophy (2017)
  • Author(s): 増田茜、増田雅美、中島宏之、小島弘幸、北村繁幸、浦丸直人、保坂卓臣、佐々木崇光、吉成浩一―
  • Journal Title: YAKUGAKU ZASSHI Volume: 137 Issue: 5 Pages: 611-622
  • DOI: 10.1248/yakushi.16-00259
  • NAID: 130005631433
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2017-05-01
  • Peer Reviewed / Open Access
• [Journal Article] Activation of nuclear receptor CAR by an environmental pollutant perfluorooctanoic acid. (2017)
  • Author(s): Abe, T., Takahashi, M., Kano, M., Amaike, Y., Ishii, C., Maeda, K., Kudoh, Y., Morishita, T., Hosaka, T., Sasaki, T., Kodama, S., Matsuzawa, A., Kojima, H., Yoshinari, K.
  • Journal Title: Arch. Toxicol. Volume: 印刷中 Issue: 6 Pages: 2365-2374
  • DOI: 10.1007/s00204-016-1888-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of p38 Mitogen-Activated Protein Kinase by Clotrimazole Induces Multidrug Resistance-Associated Protein 3 Activation through a Novel Transcriptional Element (2016)
  • Author(s): Sasaki T, Inami K, Numata Y, Funakoshi K, Yoshida M, Kumagai T, Kanno S, Matsui S, Toriyabe T, Yamazoe Y, Yoshinari K, Nagata K
  • Journal Title: J Pharmacol Exp Ther Volume: 31 Issue: 1 Pages: 102-109
  • DOI: 10.1124/jpet.115.231589
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] レポーター遺伝子導入培養細胞を用いた健康食品によるCYP1A1/1A2誘導の網羅的評価 (2016)
  • Author(s): 熊谷 健, 佐々木崇光, 永田 清
  • Journal Title: 医療薬学 Volume: 42 Pages: 701-708
  • NAID: 130006163861
  • Peer Reviewed
• [Journal Article] CYP2A13 Genetic Polymorphisms in Relation to the Risk of Bladder Cancer in Japanese Smokers (2016)
  • Author(s): Kumondai M, Hosono H, Orikasa K, Yoichi A, Sugimura H, Ozono S, Sugiyama T, Takayama T, Sasaki T, Hirasawa N, Hiratsuka M
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 39 Issue: 10 Pages: 1683-1686
  • DOI: 10.1248/bpb.b16-00422
  • NAID: 130005598497
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Indirubin, a component of Ban-Lan-Gen, activates CYP3A4 gene transcription through the human pregnane X receptor (2016)
  • Author(s): Kumagai T, Aratsu Y, Sugawara R, Sasaki T, Miyairi S, Nagata K
  • Journal Title: Drug Metab Pharmacokinet Volume: 31 Issue: 2 Pages: 139-145
  • DOI: 10.1016/j.dmpk.2016.01.002
  • NAID: 50014431254
  • Peer Reviewed
• [Journal Article] Genetic Polymorphisms of <i>CYP2A6</i> in a Case-Control Study on Bladder Cancer in Japanese Smokers (2016)
  • Author(s): Kumondai M, Hosono H, Orikasa K, Yoichi A, Sugimura H, Ozono S, Sugiyama T, Takayama T, Sasaki T, Hirasawa N, Hiratsuka M
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 39 Issue: 1 Pages: 84-89
  • DOI: 10.1248/bpb.b15-00604
  • NAID: 130005116502
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Development of a highly reproducible system to evaluate inhibition of cytochrome P450 3A4 activity by natural medicines. (2015)
  • Author(s): Sato Y, Sasaki T, Takahashi S, Kumagai T, Nagata K
  • Journal Title: J Pharm Pharm Sci Volume: 18 Issue: 4 Pages: 316-327
  • DOI: 10.18433/j3vk5g
  • Peer Reviewed
• [Journal Article] CYP2A6 genetic polymorphism is associated with decreased susceptibility to squamous cell lung cancer in Japanese smokers (2015)
  • Author(s): Hosono H, Kumondai M, Arai T, Sugimura H, Sasaki T, Hirasawa N, Hiratsuka M
  • Journal Title: Drug Metab Pharmacokinet Volume: 30 Issue: 4 Pages: 263-268
  • DOI: 10.1016/j.dmpk.2015.04.002
  • NAID: 50014429961
  • Peer Reviewed
• [Journal Article] Suppression of Cytochrome P450 3A4 Function by UDP-Glucuronosyltransferase 2B7 through a Protein-Protein Interaction: Cooperative Roles of the Cytosolic Carboxyl-Terminal Domain and the Luminal Anchoring Region. (2015)
  • Author(s): Miyauchi Y, Nagata K, Yamazoe Y, Mackenzie PI, Yamada H, Ishii Y
  • Journal Title: Mol Pharmacol Volume: 88 Issue: 4 Pages: 800-812
  • DOI: 10.1124/mol.115.098582
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Simultaneous evaluation of human CYP3A4 and ABCB1 induction by reporter assay in LS174T cells, stably expressing their reporter genes (2015)
  • Author(s): Inami K, Sasaki T, Kumagai T, Nagata K
  • Journal Title: Biopharm Drug Dispos. Volume: 36 Issue: 3 Pages: 139-147
  • DOI: 10.1002/bdd.1927
  • Peer Reviewed
• [Journal Article] 培養細胞を用いた健康食品と医薬品との薬物相互作用の薬物相互作用の網羅的調査 (2015)
  • Author(s): 熊谷 健、佐々木崇光、佐藤 裕、髙橋昌悟、細川正清、頭金正博、松永民秀、大森 栄、永田 清
  • Journal Title: 東京都病院薬剤師会雑誌 Volume: 64 Pages: 15-22
• [Journal Article] 健康食品と医薬品の併用は薬物相互作用をおこす？ (2014)
  • Author(s): 永田 清、熊谷 健、 佐々木崇光
  • Journal Title: 医薬品相互作用研究 Volume: 38 Pages: 23-30
• [Journal Article] Histone deacetylase inhibitor valproic acid promotes the differentiation of human induced pluripotent stem cells into hepatocyte-like cells. (2014)
  • Author(s): Kondo Y, Iwao T, Yoshihashi S, Mimori K, Ogihara R, Nagata K, Kurose K, Saito M, Niwa T, Suzuki T, Miyata N, Ohmori S, Nakamura K, Matsunaga T.
  • Journal Title: PLoS One. Volume: 9 Issue: 8 Pages: 1-11
  • DOI: 10.1371/journal.pone.0104010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 調剤薬局来局者を対象とした健康食品の使用実態調査とその情報に基づいたCYP2D6活性阻害評価 (2014)
  • Author(s): 佐々木崇光, 熊谷 健, 佐々木瞳, 稲見敬太, 佐藤 裕, 髙橋昌悟, 松永民秀, 頭金正博, 細川正清, 大森 栄, 永田 清
  • Journal Title: 医療薬学 Volume: 40 Pages: 488-499
  • NAID: 130005098367
  • Peer Reviewed
• [Journal Article] An Efficient Method for Differentiation of Human Induced Pluripotent Stem Cells into Hepatocyte-like Cells Retaining Drug Metabolizing Activity (2014)
  • Author(s): Kondo Y, Iwao T, Nakamura K, Sasaki T, Takahashi S, Kamada N, Matsubara T, Gonzalez FJ, Akutsu H, Miyagawa Y, Okita H, Kiyokawa N, Toyoda M, Umezawa A, Nagata K, Matsunaga T, Ohmori S
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 29 Issue: 3 Pages: 237-243
  • DOI: 10.2133/dmpk.DMPK-13-RG-104
  • NAID: 130004463382
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Differentiation of Human Induced Pluripotent Stem Cells into Functional Enterocyte-like Cells Using a Simple Method (2014)
  • Author(s): Iwao T et al.
  • Journal Title: Drug Metabolism and Pharmacokinetics Volume: 29 Issue: 1 Pages: 44-51
  • DOI: 10.2133/dmpk.DMPK-13-RG-005
  • NAID: 130004463343
  • ISSN: 1347-4367, 1880-0920
  • Peer Reviewed
• [Journal Article] Alteration of the function of the UDP-glucuronosyltransferase 1A subfamily by cytochrome P450 3A4: different susceptibility for UGT isoforms and UGT1A1/7 variants (2014)
  • Author(s): Ishii Y, Koba H, Kinoshita K, Oizaki T, Iwamoto Y, Takeda S, Miyauchi Y, Nishimura Y, Egoshi N, Taura F, Morimoto S, Ikushiro S, Nagata K, Yamazoe Y, Mackenzie PI, Yamada H
  • Journal Title: Drug Metabolism and Disposition Volume: 42 Issue: 2 Pages: 229-238
  • DOI: 10.1124/dmd.113.054833
  • Peer Reviewed
• [Presentation] 化学物質のヒトP450阻害活性とその肝障害発症との関連性 (2017)
  • Author(s): 佐々木崇光、渡邉美智子、清水佑記、櫛田まどか、隠岐仁美、竹下潤一、保坂卓臣、吉成浩一
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] インビボ毒性試験データを用いた核内受容体PXRおよびCARの新規機能の探索 (2017)
  • Author(s): 橘内陽子、竹下潤一、佐々木崇光、保坂卓臣、吉成浩一
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] 結合DNA配列の相違がPXR依存的転写活性化に及ぼす影響 (2017)
  • Author(s): 保坂卓臣、山崎悠里子、曽田勇介、佐々木崇光、吉成浩一
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] オボムコイドカラムを用いる光学活性医薬品肝代謝物の高感度LC-MS/MS分析 (2017)
  • Author(s): 工藤悠翔，中村美鈴，轟木堅一郎，佐々木崇光，清水佑記，和田圭右，大槻秀幸，水野 初，閔 俊哲，吉成浩一，豊岡利正
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] 分子記述子と階層的クラスタリングを利用したラット肝毒性のインシリコ予測の試み (2016)
  • Author(s): 吉成浩一、中山晴香、橘内陽子、隠岐仁美、保坂卓臣、佐々木崇光、竹下潤一
  • Organizer: 第29回日本動物実験代替法学会
  • Place of Presentation: 福岡
  • Year and Date: 2016-11-16
• [Presentation] 薬物代謝酵素シトクロムP450阻害活性を指標とした化学物質の生物学的プロファイリング (2016)
  • Author(s): 渡邉美智子、清水佑記、櫛田まどか、隠岐仁美、竹下潤一、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 第29回日本動物実験代替法学会
  • Place of Presentation: 福岡
  • Year and Date: 2016-11-16
• [Presentation] 新規マウスPXR活性化物質のセルベーススクリーニング (2016)
  • Author(s): 清水佑記、渡邉美智子、土屋早希、加納誠人、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 日本薬物動態学会 第31回年会
  • Place of Presentation: 松本
  • Year and Date: 2016-10-13
• [Presentation] ヒト肝細胞でのCYP3A4誘導予測におけるインシリコモデルとヒトPXRレポーターアッセイの比較 (2016)
  • Author(s): 保坂卓臣、永井美香、佐々木崇光、今野芳浩、吉成浩一
  • Organizer: 日本薬物動態学会 第31回年会
  • Place of Presentation: 松本
  • Year and Date: 2016-10-13
• [Presentation] 薬物代謝を考慮したPPARαレポーターアッセイ系の構築 (2016)
  • Author(s): 若月綾乃、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] 化学物質のヒトシトクロムP450阻害活性の網羅的評価 (2016)
  • Author(s): 渡邉美智子、清水佑記、櫛田まどか、隠岐仁美、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] マウスPXRの新規活性化物質の探索 (2016)
  • Author(s): 加納誠人、阿部太紀、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] インビトロにおけるマウス肝細胞のCAR依存的な細胞増殖 (2016)
  • Author(s): 天池優斗、阿部太紀、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] 腸管由来LS180細胞とヒト肝細胞におけるCYP3A4誘導の比較 (2016)
  • Author(s): 鶴田聡志、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] CYP3A4遺伝子の基本的転写活性化に関わる新規シスエレメントの探索 (2016)
  • Author(s): 小田桐玲生、佐々木崇光、熊谷健、永田清
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] CYP26A1レポーター遺伝子発現ウイルスを用いた低濃度下におけるレチノイン酸合成・代謝酵素活性阻害評価系の検討 (2016)
  • Author(s): 塩谷安奈里、渡邊美智子、渡辺郁実、角田駿哉、若生俊也、佐々木崇光、熊谷健、永田清
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] 多環芳香族炭化水素によるCYP2D6発現誘導機構の解析 (2016)
  • Author(s): 稲見敬太、佐々木崇光、熊谷健、吉成浩一、永田清
  • Organizer: 平成28年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 静岡
  • Year and Date: 2016-09-17
• [Presentation] オボムコイドカラムとLC-MS/MSを組み合せた光学活性医薬品の肝代謝物網羅的解析 (2016)
  • Author(s): 工藤悠翔、中村美鈴、轟木健一郎、佐々木崇光、清水佑記、和田圭右、大槻秀幸、水野初、閔俊哲、吉成浩一、豊岡利正
  • Organizer: 日本分析化学会年会第65年会
  • Place of Presentation: 札幌
  • Year and Date: 2016-09-14
• [Presentation] 健康食品による薬物代謝P450活性阻害の網羅的評価 (2016)
  • Author(s): 永田清、佐藤裕、熊谷健、佐々木崇光
  • Organizer: フォーラム2016衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2016-09-10
• [Presentation] マウスPXRの新規活性化物質の探索 (2016)
  • Author(s): 加納誠人、阿部太紀、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: フォーラム2016衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2016-09-10
• [Presentation] 肝障害性化学物質のヒトシトクロムP450阻害活性 (2016)
  • Author(s): 渡邉美智子、清水佑記、櫛田まどか、隠岐仁美、竹下潤一、 保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: フォーラム2016衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2016-09-10
• [Presentation] ラット反復投与毒性試験データを用いた肝細胞肥大の毒性学的特徴の解明 (2016)
  • Author(s): 増田茜、増田雅美、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-06-29
• [Presentation] 核内受容体PXRと臓器サイズ制御シグナルHippo pathwayのクロストーク (2016)
  • Author(s): 阿部太紀、保坂卓臣、佐々木崇光、児玉進、松沢厚、吉成浩一
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-06-29
• [Presentation] 新規マウスPXR活性化物質の探索 (2016)
  • Author(s): 加納誠人、阿部太紀、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-06-29
• [Presentation] 環境汚染物質perfluorocarboxylic acids (PFCAs) によるマウス及びヒトCAR活性化作用 (2016)
  • Author(s): 髙橋美玲、阿部太紀、小島弘幸、保坂卓臣、佐々木崇光、吉成浩一
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-06-29
• [Presentation] New approach for the prediction of repeated-dose toxicity using in vitro and in silico methods in combination with in vivo toxicity database (2016)
  • Author(s): Kouichi YOSHINARI, Hitomi OKI, Takamitsu Sasaki, Takuomi Hosaka, Jun-ichi TAKESHITA
  • Organizer: ICCA-LRI and NIHS Workshop
  • Place of Presentation: 淡路島
  • Year and Date: 2016-06-15
  • Int'l Joint Research
• [Presentation] Environmental pollutants PFCAs are phenobarbital-like indirect human and mouse CAR activators (2016)
  • Author(s): Mirei Takahashi, Taiki Abe, Hiroyuki Kojima, Takuomi Hosaka, Takamitsu Sasaki, Kouichi Yoshinari
  • Organizer: 11th International ISSX Meeting
  • Place of Presentation: Busan
  • Year and Date: 2016-06-12
  • Int'l Joint Research
• [Presentation] PXR stimulates the growth factor-mediated hepatocyte proliferation by inhibiting FOXO-mediated transcription of cell cycle suppressor genes (2016)
  • Author(s): Taiki Abe, Ryota Shizu, Satoshi Benoki, Miki Takahashi, Susumu Kodama, Masaaki Miyata, Hosaka Takuomi, Takamitsu Sasaki, Atsushi Matsuzawa, Kouichi Yoshinari
  • Organizer: 11th International ISSX Meeting
  • Place of Presentation: Busan
  • Year and Date: 2016-06-12
  • Int'l Joint Research
• [Presentation] Activation of p38 MAPK by clotrimazole enhances the transctiption of human MRP3 through a novel transcriptional element (2016)
  • Author(s): Takamitsu Sasaki, Keita Inami, Takeshi Kumagai, Kouichi Yoshinari, Kiyoshi Nagata
  • Organizer: 11th International ISSX Meeting
  • Place of Presentation: Busan
  • Year and Date: 2016-06-12
  • Int'l Joint Research
• [Presentation] ヒト肝P450発現模倣細胞を利用した薬物代謝活性評価系の確立 (2016)
  • Author(s): 佐々木 崇光、佐藤 裕、熊谷 健、吉成 浩一、永田 清
  • Organizer: 第23回HAB研究機構学術年会
  • Place of Presentation: つくば
  • Year and Date: 2016-06-01
  • Int'l Joint Research
• [Presentation] Construction of cell evaluation systems that mimic the P450 activity in human hepatocytes (2015)
  • Author(s): Sato Y, Sasaki T, Arai Y, Kumagai T, Nagata K
  • Organizer: 30 th, JSSX annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2015-11-12
• [Presentation] Protein-protein interaction of cytochrom P450 (CYP) 3A4 and UDP-glucuronosyltransferase (UGT) 1A7 in living cells: functional interaction and fluorescent resonance energy transfer (FRET) analysis (2015)
  • Author(s): Egoshi N, Kinoshita K, Koba H, Ikushiro S, Nagata K, Yamazoe Y, Peter IM, Yamada H, Ishii Y
  • Organizer: 30 th, JSSX annual meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2015-11-12
• [Presentation] Profiles of immune cells and reproduction of immune-mediated drug-induced injury (2015)
  • Author(s): Fujisaka Y, Kondo Y, Sasaki T, Kogure T, Inoue J, Nakagome Y, Wakui Y, Iwata T, Morosawa T, Umetu T, Suzuki H, Yamaguchi H, Mano N, Nagata K, Shimosegawa T
  • Organizer: American College of Gastroenterology 2015 annual scientific meeting
  • Place of Presentation: Honolulu
  • Year and Date: 2015-10-16
  • Int'l Joint Research
• [Presentation] Profiles of immune in peripheral blood and reproduction of immune-mediated drug-induced injury in vitro (2015)
  • Author(s): Fujisaka Y, Kondo Y, Kogure T, Inoue J, Nakagome Y, Iwata T, Morosawa T, Umetu T, Suzuki H, Yamaguchi H, Sasaki T, Mano N, Nagata K, Shimosegawa T
  • Organizer: 第19回日本肝臓学会
  • Place of Presentation: 東京
  • Year and Date: 2015-10-08
• [Presentation] レチノイン酸代謝酵素CYP26の活性阻害評価系構築 (2015)
  • Author(s): 新井 悠、佐々木崇光、稲見敬太、佐藤 裕、熊谷 健、吉成浩一、永田 清
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 岩手
  • Year and Date: 2015-09-26
• [Presentation] レポーター遺伝子導入細胞株を用いた健康食品によるCYP1A1/1A2誘導の網羅的評価 (2015)
  • Author(s): 永田 清、佐々木崇光、熊谷 健
  • Organizer: フォーラム2015 衛生薬学・環境トキシコロジー
  • Place of Presentation: 神戸
  • Year and Date: 2015-09-17
• [Presentation] サイクリン依存性キナーゼ1(Cdk1)がCYP2D6及びCYP3A4遺伝子発現に及ぼす影響の解析 (2015)
  • Author(s): 稲見敬太、佐々木崇光、中島菜月、小野 緑、熊谷 健、永田 清
  • Organizer: 平成27年度内外環境応答・代謝酵素研究会
  • Place of Presentation: 旭川
  • Year and Date: 2015-07-18
• [Presentation] 薬物代謝酵素発現細胞及び誘導評価細胞を用いた薬物性肝障害研究について (2015)
  • Author(s): 佐々木崇光、熊谷 健、永田 清
  • Organizer: 第42回日本毒性学会学術年会
  • Place of Presentation: 金沢
  • Year and Date: 2015-06-29
  • Invited
• [Presentation] Cyclin-dependent kinase 1 involved in regulation of CYP2D6 expression. (2014)
  • Author(s): Inami K, Sasaki T, Kanno S, Kumagai T, Nagata K
  • Organizer: 19th North American ISSX Meeting and 29th JSSX Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-10-19 – 2014-10-23
• [Presentation] A novel evaluation system for inhibition of CYP3A4 activity by dietary supplements. (2014)
  • Author(s): Sato Y, Takahashi S, Sasaki T, Kumagai T, Nagata K
  • Organizer: 19th North American ISSX Meeting and 29th JSSX Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-10-19 – 2014-10-23
• [Presentation] Suppression of cytochrome P450 3A4 actiivity by UDP-glucuronosyltaransferase (UGT) 2B7: The role of charged residue(s) in the cytosolic tail of UGT2B7 (2014)
  • Author(s): Miyauchia Y, Ishiia Y, Nagata K, Yamazoe Y, Mackenzie PI, Yamada H
  • Organizer: 19th North American ISSX Meeting and 29th JSSX Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-10-19 – 2014-10-23
• [Presentation] Effect of Ban-Lan-Gen on transactivation of the CYP1A1 and CYP1A2 genes A novel evaluation system for inhibition of CYP3A4 activity by dietary supplements (2014)
  • Author(s): Kumagai T, Kiyomatsu T , Sasaki T, Nagata K
  • Organizer: 19th North American ISSX Meeting and 29th JSSX Meeting
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-10-19 – 2014-10-23
• [Presentation] 板藍根含有成分のCYP1A1/1A2遺伝子発現誘導に及ぼすPXRの影響 (2014)
  • Author(s): 熊谷 健、清松孝行、佐々木崇光、永田 清
  • Organizer: 創薬研究センターシンポジウム
  • Place of Presentation: 東北薬科大学（宮城県仙台市）
  • Year and Date: 2014-06-14
• [Presentation] AhRを介さない多環芳香族炭化水素によるCYP誘導機構の解明 (2014)
  • Author(s): 稲見敬太、佐々木崇光、菅野秀一、熊谷 健、永田 清
  • Organizer: 創薬研究センターシンポジウム
  • Place of Presentation: 東北薬科大学（宮城県仙台市）
  • Year and Date: 2014-06-14
• [Presentation] アデノウイルスを用いた新規CYP3A4活性阻害実験系の開発と健康食品による薬物代謝活性阻害評価 (2014)
  • Author(s): 佐藤 裕、髙橋昌悟、佐々木崇光、熊谷 健、永田 清
  • Organizer: 創薬研究センターシンポジウム
  • Place of Presentation: 東北薬科大学（宮城県仙台市）
  • Year and Date: 2014-06-14
• [Presentation] Pharmacokinetic function of hepatocyte-like cells differentiated from human iPS cells (2013)
  • Author(s): Kondo Y, Iwao T, Ogihara R, Sasaki T, Nagata K, Kurose K, Horikawa T, Niwa T, Yamaori S, Ohmori S, Nakamura K, Matsunaga T
  • Organizer: 28th JSSX annual meeting
  • Place of Presentation: Tokyo
• [Presentation] Introduction of a N-glycosylation site to UDP-glucurosyltransferase 2B3 alters the sensitivity to cytochrome P450 3A1-dependent modulation (2013)
  • Author(s): Nakamura T, Miyauchi Y, Takeda T, Yamazoe Y, Nagata K, Mackenzie P, Yamada H, Ishii Y
  • Organizer: 28th JSSX annual meeting
  • Place of Presentation: Tokyo
• [Presentation] Cytochrome P450 3A4 restores the catalytic property of udp-glucuronosyltransferase 1A1*6, one of the allelic variants which are concerned for causing gilbert syndrome (2013)
  • Author(s): Kinoshita K, Oizaki T, Iwamoto Y, Nishimura Y, Miyauchi Y, Ikushiro S, Yamazoe Y, Nagata K, Mackenzie P, Yamada H, Ishii Y
  • Organizer: 28th JSSX annual meeting
  • Place of Presentation: Tokyo
• [Presentation] Suppression of cytochrome p450 3A4 activity By UDP-glucuronosyltransferase 2B7: crucial role of the length of UGT2B7 cytosolic tail in the suppression (2013)
  • Author(s): Miyauchi Y, Nagata K, Yamazoe Y, Mackenzie P, Yamada H, Ishii Y
  • Organizer: 28th JSSX annual meeting
  • Place of Presentation: Tokyo
• [Presentation] CYP2D6活性阻害評価系の構築と健康食品の使用実態調査に基づいた薬物相互作用の解析 (2013)
  • Author(s): 佐々木瞳、佐々木崇光、稲見敬太、熊谷 健、永田 清
  • Organizer: 第23回日本医療薬学会年会
  • Place of Presentation: 仙台
• [Presentation] 新規P450活性阻害実験系の開発と健康食品による薬物代謝活性阻害評価 (2013)
  • Author(s): 佐藤 裕、髙橋昌悟、石橋幸奈、根本あゆみ、齋藤詩奈子、角間元美、榊 聡美、伏見 彩、佐々木崇光、熊谷 健、永田 清
  • Organizer: フォーラム2013衛生薬学・環境トキシコロジー
  • Place of Presentation: 福岡
• [Presentation] ヒトiPS細胞由来肝細胞を用いた薬物代謝酵素誘導評価 (2013)
  • Author(s): 松永民秀、近藤祐樹、荻原留理、岩尾岳洋、永田 清、黒瀬光一、堀川隆司、丹波卓朗、山折 大、大森 栄、中村克徳
  • Organizer: フォーラム2013衛生薬学・環境トキシコロジー
  • Place of Presentation: 福岡
• [Presentation] CYP3A4遺伝子発現誘導に及ぼす健康食品の影響 (2013)
  • Author(s): 熊谷 健、中澤洋一、工藤裕太、齋藤雄大、竹屋里恵、笠原 彩、佐々木崇光、永田 清
  • Organizer: フォーラム2013衛生薬学・環境トキシコロジー
  • Place of Presentation: 福岡
• [Presentation] 肝分化iPS細胞へのLETF同時導入によるCYP3A4発現影響 (2013)
  • Author(s): 佐藤 裕、高橋昌悟、佐々木崇光、松永民秀、永田 清
  • Organizer: 2013年度P450、UGT、SULT研究会
  • Place of Presentation: 宮崎
• [Presentation] 多環芳香族炭化水素によるCYP2D6およびCYP3A4の発現調節機構の解析 (2013)
  • Author(s): 稲見敬太、佐々木崇光、熊谷 健、菅野秀一、永田 清
  • Organizer: 2013年度P450、UGT、SULT研究会
  • Place of Presentation: 宮崎
• [Presentation] MRP3遺伝子発現誘導に関与するMAPK経路の解析 (2013)
  • Author(s): 稲見敬太、、佐々木崇光、沼田喜弘、船越広大、新井 悠、菅野秀一、熊谷 健、永田 清
  • Organizer: 創薬研究センターシンポジウム
  • Place of Presentation: 仙台
• [Presentation] 健康食品である板藍根のCYP3A4発現誘導に及ぼす影響 (2013)
  • Author(s): 熊谷 健、菅原亮輔、三浦真知、佐々木崇光、宮入伸一、永田 清
  • Organizer: 創薬研究センターシンポジウム
  • Place of Presentation: 仙台
• [Book] エンドトキシン・自然免疫研究 17 (2014)
  • Author(s): 坂口修平、永田 清
  • Total Pages: 105
  • Publisher: 医学図書出版
• [Book] 敗血症の診断・治療の実情と病態・メカニズムをふまえた開発戦略 (2013)
  • Author(s): 坂口修平、永田 清
  • Total Pages: 12
  • Publisher: 技術情報協会