Characterization of the NS1 mutants induced by a chemical substance
Project/Area Number |
25460206
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Medical pharmacy
|
Research Institution | Chubu University |
Principal Investigator |
HAYASHI Kyoko 中部大学, 生命健康科学研究所, 研究員 (60110623)
|
Co-Investigator(Kenkyū-buntansha) |
LEE Jung-Bum 富山大学, 大学院医学薬学研究部(薬学), 助教 (40332655)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | インフルエンザウイルス / スフィドロフラン誘導体 / 変異ウイルス / 病原性 / NS1蛋白 / 遺伝子解析 / NS1 蛋白 / NS1タンパク |
Outline of Final Research Achievements |
An influenza virus mutant induced by treating the virus with a sphydrofuran derivative (MFPT) was found to possess attenuated pathogenicity when compared with that of non-treated parent virus. Sequence analysis revealed that a mutation, P164S in NS1 protein was detected in the mutant. Nucleoprotein was found to be retained in the nuclei in the mutant virus-infected cells. It can be shown that the mutation might be one of the key residues to control NS1 function, and as a result, might be a favorable mutation in the ns gene for the attenuation of influenza virus. When the sphydrofuran derivative was intravaginally applied to mice, the virus yields decreased dose-dependently against both herpes simplex virus types 1 and 2.
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Report
(4 results)
Research Products
(2 results)