Reconstruction of vaginal microbiota to prevent and treat miscarriage and premature birth
| Project/Area Number |
25460226
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Medical pharmacy
|
|---|
| Research Institution | Meiji Pharmaceutical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 膣内細菌叢 / 妊婦 / Lactobacillus / 膣細菌 / LAMP / 迅速検出法 / 細菌性膣症 / Gardnerella / 膣 / 微生物叢 / パイロシーケンス / qPCR / Lactobacilus spp. |
|---|
| Outline of Final Research Achievements |
Miscarriage and premature birth occur due to an unbalance in the vaginal microbiota. Therefore, novel prevention and therapeutic method should be ble to reconstruct the unbalance in vaginal microbiota. During the last three years, we elucidated the following three issues; 1) Predominant Lactobacillus sp. 1 in pregnant women was identified by comprehensively analyzing vaginal microbiota using pyrosequencing method. 2) Cytotoxicity due to Gardnerella sp. was reduced in the presence of Lactobacillus sp. 1 on HeLa cell and also Lactobacillus sp. 1 inhibited the production of inflammatory cytokine. 3) LAMP (Loop-Mediated Isothermal Amplification) method was developed in order to detect Gardnerella sp. with high sensitivity and rapidly.
These findings suggested that Lactobacillus sp. 1 has become a candidate of probiotics to prevent or treat bacterial vaginosis.
|
Report
(4 results)
Research Products
(30 results)
-
-
[Journal Article] Selection of orthologous genes for construction of a highly resolved phylogenetic tree and clarification of the phylogeny of Trichosporonales species.2015
Author(s)
Takashima, M., Manabe, R., Iwasaki, W., Ohyama, A., Ohkuma, M., and Sugita, T.
-
Journal Title
PLOS ONE
Volume: 10
Issue: 8
Pages: e0131217-e0131217
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
