Functional analysis of Dlg1 in the extension and fusion of developing tissues.

• Project/Area Number: 25460240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Gunma University
• Principal Investigator: Kogo Akiko 群馬大学, 大学院医学系研究科, 講師 (20340242)
• Co-Investigator(Kenkyū-buntansha): 向後 寛 群馬大学, 大学院医学系研究科, 講師 (20282387) ; 松崎 利行 群馬大学, 大学院医学系研究科, 教授 (30334113) ; 野村 隆士 藤田保健衛生大学, 医学部, 講師 (20325161) ; 下村 敦司 北海道医療大学, リハビリテーション科学部, 教授 (50340237)
• Research Collaborator: NEMOTO Kanako ; IMAI Airi
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 心臓 / コルチ器 / 収斂伸長 / 細胞運動 / DLG1 / 細胞間ジャンクション / 発生 / 聴覚上皮 / ミオシンVI / 非筋肉型ミオシン / 上皮 / convergent extension / 平面内極性 / 器官形成 / ノックアウトマウス / 細胞極性 / 収斂的伸長 / マウス

Outline of Final Research Achievements

DLG1 is a scaffolding protein that anchors various proteins near the plasma membrane. Null mutant mice for Dlg1 gene exhibits congenital defects in many organs. To clarify the function of DLG1 protein, developmental process was examined in the outflow tract of the heart and the organ of Corti in the inner ear, whose developments are impaired in the mutant. In the development of outflow tract, lengthening of the truncus arteriosus was not enough in the mutant. This result implicates defects in the cell migration process from the second heart field to the nascent outflow tract region. In the organ of Corti, loss of function of the DLG1 protein caused abnormal patterns in cell-cell junction remodeling and rearrangement of epithelial cells during the tissue elongation. These results showed that DLG1 regulates cell motility which is necessary for the tissue extension.

Research Products

• [Journal Article] Requirement of DLG1 for cardiovascular development and tissue elongation 1 during cochlear, enteric, and skeletal development: possible role in convergent extension. (2015)
  • Author(s): Akiko Iizuka-Kogo, Takao Senda, Tetsu Akiyama, Atsushi Shimomura, Ryuji Nomura, Yoshimi Hasegawa, Ken-ichi Yamamura, Hiroshi Kogo, Nobuhiko Sawai, Toshiyuki Matsuzaki
  • Journal Title: PLoS ONE Volume: 10 Issue: 4 Pages: e0123965-e0123965
  • DOI: 10.1371/journal.pone.0123965
  • Peer Reviewed / Open Access
• [Journal Article] Targeted deletion of the C-terminus of the mouse adenomatous polyposis coli tumor suppressor results in neurologic phenotypes related to schizophrenia (2014)
  • Author(s): Takanori Onouchi, Katsunori Kobayashi, Kazuyoshi Sakai, Atsushi Shimomura, Ron Smits, Chiho Sumi-Ichinose, Masafumi Kurosumi Keizo Takao, Ryuji Nomura, Akiko Iizuka-Kogo, Hidenori Suzuki,Kazunao Kondo, Tetsu Akiyama Tsuyoshi Miyakawa Riccardo Fodde and Takao Senda, Riccardo Fodde, Takao
  • Journal Title: Molecular Brain Volume: 7 Issue: 1 Pages: 21-21
  • DOI: 10.1186/1756-6606-7-21
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands (2013)
  • Author(s): Susa T, Sawai N, Aoki T, Iizuka-Kogo A, Kogo H, Negishi A, Yokoo S, Takata K, Matsuzaki T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 46 Issue: 6 Pages: 187-197
  • DOI: 10.1267/ahc.13037
  • NAID: 130003381873
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Journal Article] Decreased apoptosis and persistence of the common nephric duct during the development of an aberrant vesicoureteral junction in dlg1 gene-targeted mice (2013)
  • Author(s): Akiko Iizuka-Kogo, Tetsu Akiyama, Takao Senda
  • Journal Title: Anat Rec. Volume: 296 Issue: 12 Pages: 1936-1942
  • DOI: 10.1002/ar.22814
  • Peer Reviewed
• [Presentation] マウスコルチ器の組織形成における細胞間ジャンクションの再編成 (2016)
  • Author(s): 向後晶子、根本奏子、今井愛理、向後寛、松崎利行
  • Organizer: 第121回日本解剖学会全国学術集会
  • Place of Presentation: ビッグパレットふくしま（郡山）
  • Year and Date: 2016-03-29
• [Presentation] Dlg1遺伝子KOマウス発生過程における内耳聴覚上皮のcell junction remodeling異常 (2015)
  • Author(s): 向後晶子、向後寛、澤井信彦、松崎利行
  • Organizer: 第38回分子生物学会年会
  • Place of Presentation: 神戸国際展示場（神戸）
  • Year and Date: 2015-12-03
• [Presentation] コルチ器の伸長における予定感覚上皮の形態学的解析 (2015)
  • Author(s): 向後晶子・向後寛・澤井信彦・松崎利行
  • Organizer: 第120回日本解剖学会全国学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-22
• [Presentation] Convergent extensionによるコルチ器の伸長とDlg1の機能 (2014)
  • Author(s): 向後晶子・向後寛・千田隆夫・松崎利行
  • Organizer: 第37回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-27
• [Presentation] Dlg1遺伝子ノックアウトマウスにおける心臓奇形の発症機構
  • Author(s): 向後晶子、向後寛、千田隆夫、松崎利行
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 下野市（自治医科大学）
• [Remarks] 群馬大学大学院医学系研究科 生体構造学
  • URL: http://anatcb.dept.med.gunma-u.ac.jp/