| Project/Area Number |
25460243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OTANI Hiroki 島根大学, 医学部, 教授 (20160533)
MATSUMOTO Akihiro 島根大学, 医学部, 助教 (70346378)
INOUE Takayuki 島根大学, 医学部, 助教 (50581386)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 中枢神経系 / マイクロRNA / 組織形成 |
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| Outline of Final Research Achievements |
We searched the microRNAs (miRNAs) which were related to histogenesis of the cerebrum in mice and analyzed their roles in the development of the cerebral cortex. We analyzed the change in expression of miRNAs. We chose let7b-5p, which showed the most increased miRNA level from E12 to E15 and miR409-3p, which showed the most decreased miRNA level in the same period. Using RNA interference to analyze the function of let7b-5b, we injected the double strand RNA of let7b-5p into the ventricle of mouse embryos on E13 using in-utero operation. Roughness around the ventricle was observed and irregularity of cell arrangement was detected around the cells in the part of cerebral cortex on E15. The most of cells in which ds RNA were detected were S phase in the cell cycle. The roles of these miRNAs were to regulate histogenesis in the cerebral cortex during embryonic period.
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