Sequential morphogenetic changes of lymphatic endothelium caused by molecular genetic cues

• Project/Area Number: 25460249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Iwate Medical University
• Principal Investigator: ISOGAI SUMIO 岩手医科大学, 医学部, 准教授 (60212966)
• Co-Investigator(Kenkyū-buntansha): SHIMODA HIROSHI 弘前大学, 医学研究科, 教授 (20274748)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リンパ管 / 小型魚類 / リンパ管発生 / 胸管 / ゼブラフィッシュ / Arrey Tomography / ミュータント / メダカ / 肺魚 / モデル動物

Outline of Final Research Achievements

As well known, lymph-endothelial cells are originated from venous endothelia in zebrafish and medaka embryos. We investigated sequential phenotypic changes which are closely related with the molecular genetic changes to become the lymph endothelium from the venous endothelium.
1. We compiled the anatomical atlas of lymph vascular systems in zebrafish and medaka from embryos through adults.2. We investigated the sequential morphogenetic changes of lymph-endothelial cells with Arrey Tomography.3. Using VEGFR3 mutant zebrafish, we analyzed developmental mechanisms of cervical lymph-sac.

Research Products

• [Journal Article] Integration of vascular systems between the brain and spinal cord in zebrafish (2015)
  • Author(s): Kimura E., Isogai S., Hitomi J
  • Journal Title: Developmental Biology Volume: 406 Pages: 40-51
  • Peer Reviewed
• [Presentation] ヒトの血管とリンパ管に残された謎を魚で解く (2015)
  • Author(s): 磯貝純夫
  • Organizer: 第24回日本形成外科学会基礎学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2015-10-08
  • Invited
• [Presentation] 小型魚類のリンパ輸送経路 (2015)
  • Author(s): 齊藤絵里奈, 磯貝純夫, 成田大一, 岡野大輔, 浅野義哉, 下田浩
  • Organizer: 第39回日本リンパ学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-27 – 2015-03-28
• [Presentation] Morphogenetic mechanisms of the lymphatic endothelial cells in zebrafish and medaka. (2015)
  • Author(s): Isogai Sumio
  • Organizer: 第120回日本解剖学会総会全国学術集会、解剖学会生理学会合同大会2015シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] Novel mechanisms involved in the endothelial differentiation of arteries and veins during vasculogenesis and angiogenesis. (2015)
  • Author(s): Erina Saito, Sumio Isogai, Eiji Kimura, Hiroshi Shimoda, Jiro Hitomi
  • Organizer: 第120回日本解剖学会総会全国学術集会、解剖学会生理学会合同大会2015シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] リンパ管内皮の始原的特性 (2015)
  • Author(s): 磯貝純夫
  • Organizer: リンパ・免疫系懇話会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-20
  • Invited
• [Presentation] Angioblast takes different way whether it becomes an artery or a vein in the endothelial differentiation and the tube formation. (2014)
  • Author(s): Sumio Isogai, Erina Saito, Eiji Kimura, Jiro Hitomi
  • Organizer: North American Vascular Biology
  • Place of Presentation: USA CA
  • Year and Date: 2014-10-18 – 2014-10-23
• [Presentation] 小型魚類リンパ管の標識法の開発 (2014)
  • Author(s): 磯貝純夫
  • Organizer: 第65回日本動物学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-09-11 – 2014-09-13
• [Book] 細胞 (2015)
  • Author(s): 磯貝純夫
  • Total Pages: 4
  • Publisher: ニューサイエンス社