| Project/Area Number |
25460255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Atsumasa 東京理科大学, 基礎工学部・生物工学科, 助教 (00535809)
NAKAJIMA Tadaaki 東京理科大学, 基礎工学部・生物工学科, 助教 (40631213)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 卵管 / 子宮 / 膣 / マイクロアレイ / 運命決定 / クローン性株細胞 / 液性因子 / 運命決定因子 |
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| Outline of Final Research Achievements |
In this study, identification of fate determining factors for oviductal, uterine and vaginal epithelium were attempted. First, transcriptome analysis was performed among developmental and adult oviducts, uteri and vaginae, and upregulated genes in developmental oviducts, uteri or vaginae were identified as candidate genes of fate determining factors. Clonal cell lines with tissue- and developmental stage-specific phenotypes were established from developmental oviductal, uterine and vaginal mesenchyme. Furthermore, 3D co-culture system was also established using the cell line for identification of the factors. Thus, the fate determining factors secreted from mesenchyme and acting into epithelium will be identified in the culture system.
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