Maturation and degeneration of oocyte compared with neuronal degeneration.

• Project/Area Number: 25460256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Takahashi Noriyuki 聖マリアンナ医科大学, 医学部, 研究技術員 (80267450)
• Co-Investigator(Kenkyū-buntansha): 石塚 文平 聖マリアンナ医科大学, 医学部, 名誉教授 (80097336)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 卵巣 / 卵成熟 / 卵変性 / 神経 / 神経変性

Outline of Final Research Achievements

The findings are that 1) no statistical differences between wild-type and NF-H KO mice were found in reproductive parameters, indicating that NF-H expression itself scarcely influence on reproductive system, 2) IDPN showed a transient reproductive toxicity on gonads of both sexes directly and low dose IDPN does not promote oocyte maturation, and 3) neuronal proteins tau and FMR1 were expressed in specific types (including oocyte) of ovarian cells.

Research Products

• [Journal Article] Cresyl violet stains mast cells selectively: Its application to counterstaining in immunohistochemistry. (2017)
  • Author(s): Takahashi N, Tarumi W, Hamada N, Ishizuka B and Itoh MT
  • Journal Title: Zoological Science Volume: 34(2) Issue: 2 Pages: 147-150
  • DOI: 10.2108/zs160162
  • NAID: 40021168652
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The stage- and cell type-specific localization of fragile X mental retardation protein in rat ovaries. (2015)
  • Author(s): Takahashi N, Tarumi W, Itoh MT and Ishizuka B
  • Journal Title: Reproductive Sciences Volume: 22(12) Issue: 12 Pages: 1524-9
  • DOI: 10.1177/1933719115589416
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The stage- and cell-type specific localization of fragile X mental retardation protein in rat ovaries (2015)
  • Author(s): Noriyuki Takahashi, Wataru Tarumi, Masanori T Itoh and Bunpei Ishizuka
  • Journal Title: Reproductive Sciences Volume: 不明
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Acute toxic effects of 3,3'-iminodipropionitrile on hypothalamic-pituitary-gonadal axis in male rats. (2014)
  • Author(s): Takahashi N, Hamada N, Ishizuka B
  • Journal Title: Reproductive Toxicology Volume: 43 Pages: 19-25
  • DOI: 10.1016/j.reprotox.2013.10.007
  • Peer Reviewed
• [Journal Article] Involvement of hyaluronan synthesis in ovarian follicle growth in rats. (2013)
  • Author(s): Takahashi N, Tarumi W, Ishizuka B
  • Journal Title: Reproduction Volume: 147 Issue: 2 Pages: 189-197
  • DOI: 10.1530/rep-13-0464
  • Peer Reviewed
• [Presentation] The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes. (2016)
  • Author(s): 高橋則行・鈴木直・伊藤正則・石塚文平
  • Organizer: 日本動物学会第87回大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] ラット排卵時におけるIDPN投与の影響 (2015)
  • Author(s): 高橋則行・樽見航・浜田直美・河村和弘・鈴木直・伊藤正則・石塚文平
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ：新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] ラット卵母細胞におけるFMRPの存在 (2015)
  • Author(s): 伊藤正則・樽見航・高橋則行・石塚文平
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ：新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] 卵胞成長におけるヒアルロン酸の必要性 (2014)
  • Author(s): 高橋則行・浜田直美・樽見航・河村和弘・田中守・鈴木直・伊藤正則・石塚文平
  • Organizer: 日本動物学会第85回仙台大会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Toxic effects of 3,3'-iminodipropionitrile on hypothalamic-pituitary-gonadal axis in male rats. (2013)
  • Author(s): Takahashi N, Hamada N and Ishizuka B
  • Organizer: The 9th Conference of the Pacific Rim Society for Fertility and Sterility
  • Place of Presentation: 神戸国際会議場
• [Presentation] 3,3'-iminodipropionitrile (IDPN)の生殖軸における毒性について (2013)
  • Author(s): 高橋則行・樽見航・浜田直美・伊藤正則・河村和弘・田中守・鈴木直・石塚文平
  • Organizer: 第58回日本生殖医学会学術講演会
  • Place of Presentation: 神戸国際会議場