In vivo measurement of developmental signaling activities during dorso-ventral patterning in zebrafish embryo
| Project/Area Number |
25460258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Fujita Health University |
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Principal Investigator |
KONDOW Akiko 藤田保健衛生大学, 総合医科学研究所, 助教 (90396838)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yoichi 東京大学, 生産技術研究所, 教授 (70302627)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNUMA Kiyoshi 長岡技術科学大学, 工学(系)研究科(研究院), 准教授 (50396834)
KOBAYASHI Tetsuya 東京大学, 生産技術研究所, 准教授 (90513359)
NONAKA Shigenori 基礎生物学研究所, 時空間制御研究室, 准教授 (90435529)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ライブイメージング / 発生学・形態形成学 / ゼブラフィッシュ / 発生生物学 |
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| Outline of Final Research Achievements |
During establishing the dorso-ventral pattern in vertebrate embryos, the timing of cells to be expose to a certain signal is known to be important. However it is not well known when and where each cell is expose to a certain signal in an embryo during development. In order to investigate the spatio-temporal change of a TGF-beta super family Bmp signaling in each cells during dorso-ventral patterning in zebrafish, we tried to develop a method to track Nodal signal activities, another TGF-beta super family member, in each cells of a zebrafish embryo. We visualized Nodal response by the localization of Smad2-Smad4 complex to nucleus, and observed it with digital scanned light sheet microscope. We were also successful in developing a novel 3D cell tracking method.
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Report
(4 results)
Research Products
(4 results)
