• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

In vivo measurement of developmental signaling activities during dorso-ventral patterning in zebrafish embryo

Research Project

Project/Area Number 25460258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionFujita Health University

Principal Investigator

KONDOW Akiko  藤田保健衛生大学, 総合医科学研究所, 助教 (90396838)

Co-Investigator(Kenkyū-buntansha) SATO Yoichi  東京大学, 生産技術研究所, 教授 (70302627)
Co-Investigator(Renkei-kenkyūsha) OHNUMA Kiyoshi  長岡技術科学大学, 工学(系)研究科(研究院), 准教授 (50396834)
KOBAYASHI Tetsuya  東京大学, 生産技術研究所, 准教授 (90513359)
NONAKA Shigenori  基礎生物学研究所, 時空間制御研究室, 准教授 (90435529)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsライブイメージング / 発生学・形態形成学 / ゼブラフィッシュ / 発生生物学
Outline of Final Research Achievements

During establishing the dorso-ventral pattern in vertebrate embryos, the timing of cells to be expose to a certain signal is known to be important. However it is not well known when and where each cell is expose to a certain signal in an embryo during development. In order to investigate the spatio-temporal change of a TGF-beta super family Bmp signaling in each cells during dorso-ventral patterning in zebrafish, we tried to develop a method to track Nodal signal activities, another TGF-beta super family member, in each cells of a zebrafish embryo. We visualized Nodal response by the localization of Smad2-Smad4 complex to nucleus, and observed it with digital scanned light sheet microscope. We were also successful in developing a novel 3D cell tracking method.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2014 2013

All Presentation (4 results)

  • [Presentation] 3D Cell Tracking under dense cell culture conditions by preserving the structure of neighbor cells2014

    • Author(s)
      Ryoma Bise, Yoichi Sato, Akiko Kondow, Tetsuya J. Kobayashi, Kiyoshi Ohnuma
    • Organizer
      IEEE International Symposium on Biomedical Imaging (ISBI) 2014
    • Place of Presentation
      北京、中華人民共和国
    • Year and Date
      2014-04-29 – 2014-05-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 全胚ライブイメージングによる形態形成の理解2013

    • Author(s)
      近藤晶子、大沼清、野中茂紀、橋本敬一郎
    • Organizer
      定量生物学の会第六回年会
    • Place of Presentation
      大阪大学銀杏会館、大阪
    • Related Report
      2013 Research-status Report
  • [Presentation] 大局的細胞移動推定及び局所的相対位置関係類似度を用いた密な状態における3次元細胞トラッキング2013

    • Author(s)
      備瀬竜馬、佐藤洋一、近藤晶子、小林徹也、大沼清
    • Organizer
      第16回画像の認識・理解シンポジウムMIRU2013
    • Place of Presentation
      国立情報学研究所、東京
    • Related Report
      2013 Research-status Report
  • [Presentation] 密な状況での細胞群の3次元追跡2013

    • Author(s)
      備瀬竜馬、佐藤洋一、近藤晶子、小林徹也、大沼清
    • Organizer
      第13回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学本郷キャンパス、東京
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi