Visualization of the entire differentiation process of murine M cells: suppression of their maturation in cecal patches.
| Project/Area Number |
25460261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kimura Shunsuke 北海道大学, 医学(系)研究科(研究院), 助教 (40444525)
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| Research Collaborator |
Mutoh Mami 北海道大学, 大学院歯学研究科・口腔医学専攻, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | パイエル板 / 濾胞上皮 / M細胞 / 粘膜免疫 / 上皮組織 |
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| Outline of Final Research Achievements |
The microfold (M) cell residing in the follicle-associated epithelium (FAE) is a specialized epithelial cell that initiates mucosal immune responses by sampling luminal antigens. The differentiation process of M cells remains unclear due to limitations of analytical methods. Here we found that M cells were classified into two functionally different subtypes based on the expression of Glycoprotein 2 (GP2) by newly developed image cytometric analysis. GP2-high M cells actively took up luminal microbeads whereas GP2-negative or low cells scarcely ingested them, suggesting that GP2-high M cells represent functionally mature M cells. Further, the GP2-high mature M cells were abundant in Peyer’s patch but sparse in the cecal patch: this was most likely due to a decrease in the nuclear translocation of RelB, a downstream transcription factor for the receptor activator of NF-κB signaling.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Potential role of the formation of tunneling nanotubes in HIV-1 spread in macrophages.2016
Author(s)
Hashimoto M, Bhuyan F, Hiyoshi M, Noyori O, Nasser H, Miyazaki M, Saito T, Kondoh Y, Osada H, Kimura S, Hase K, Ohno H, Suzu S
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Journal Title
J Immunol
Volume: 196
Issue: 4
Pages: 1832-1841
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues2014
Author(s)
Yuuki Obata, Shunsuke Kimura, Gaku Nakato, Keito Iizuka, Yurika Miyagawa, Yutaka Nakamura, Yukihiro Furusawa, Machiko Sugiyama, Keiichiro Suzuki, Masashi Ebisawa, Yumiko Fujimura, Hisahiro Yoshida, Toshihiko Iwanaga, Koji Hase and Hiroshi Ohno.
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Journal Title
EMBO Reports
Volume: 12
Issue: 12
Pages: 1297-1304
DOI
Related Report
Peer Reviewed
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