Tumor cell dynamism and molecular mechanism invloved in tumor vascular metastasis in novel cancer research model of zebrafish

• Project/Area Number: 25460262
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Hirosaki University
• Principal Investigator: Shimoda Hiroshi 弘前大学, 医学(系)研究科(研究院), 教授 (20274748)
• Co-Investigator(Kenkyū-buntansha): ISOGAI SUMIO 岩手医科大学, 医学部, 准教授 (60212966)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ゼブラフィッシュがんモデル / 腫瘍脈管新生 / 生体イメージング / がん血管・リンパ管性転移 / 生体ライブイメージング / 腫瘍リンパ管・血管新生

Outline of Final Research Achievements

A novel cancer research model of transgenic zebrafishes, which allowed a precise analysis of cancer cell dynamism for metastasis through blood and lymphatic vasculature, was developed. Live imaging of human tumor cells in the metastasis model unveiled some unique patterns of tumor vascular invasion and of motion of tumor cells through vasculature. The zebrafish implanted with human pancreatic cancer cells producing VEGF-A often demonstrated focal growth of vascular endothelial cells at tumor invasion sites. The molecular mechanisms involved in tumor vascular invasion including microangiogenesis and/or lymphangiogenesis is under investigation.
達成度

Research Products

• [Presentation] Lymphatic transport pathway in small fish (2015)
  • Author(s): ⑥Erina Saito, Sumio Isogai, Hirokazu Narita, Daisuke Okano, Hiroshi Shimoda
  • Organizer: 25th World Congress of Lymphology
  • Place of Presentation: SanFrancisco, USA
  • Year and Date: 2015-09-07
  • Int'l Joint Research
• [Presentation] Novel mechanisms involved in the endothelial differentiation of arteries and veins (2015)
  • Author(s): 斉藤絵里奈、磯貝純夫、木村栄治、下田浩、人見次郎
  • Organizer: 第１２０回日本解剖学会総会・学術集会、第92回日本生理学会大会合同集会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] 小型魚類の機能形態学的解析 (2014)
  • Author(s): 斉藤絵里奈、出口友則、磯貝純夫、成田大一、岡野大輔、浅野義哉、下田浩
  • Organizer: 第55回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 松本市中央公民館
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] 小型魚類リンパ管の組織化学的解析 (2014)
  • Author(s): 齊藤絵里奈、出口友則、磯貝純夫、出口友則、成田大一、岡野大輔、浅野義哉、下田浩
  • Organizer: 第119回日本解剖学会総会・学術集会
  • Place of Presentation: 自治医科大学キャンパス（栃木県下野市）
• [Presentation] 小型魚類におけるリンパ管の発生過程と機能形態 (2013)
  • Author(s): 齊藤絵里奈、磯貝純夫、出口友則、成田大一、浅野義哉、下田浩
  • Organizer: 第54回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 航空会館（東京・新橋）
• [Presentation] 小型魚類の血管・リンパ管系の構築とがん移植モデルの確立
  • Author(s): 下田浩、齊藤絵里奈、渡邉誠二、浅野義哉、谷利樹、岡野大輔、成田大一、外崎敬和
  • Organizer: 弘前医学会
  • Place of Presentation: 弘前大学メディカルコミュニケーションセンター（青森県弘前市）