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Elucidating the role of Ras downstream pathways and Ras signal strength in angio/lymphangiogenesis and epidermal morphogenesis

Research Project

Project/Area Number 25460263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionThe University of Tokyo

Principal Investigator

ICHISE Hirotake  東京大学, 医科学研究所, 講師 (10313090)

Co-Investigator(Kenkyū-buntansha) ICHISE Taeko  東京大学, 医科学研究所, 助教 (00396863)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsマウス / Ras / Erk / リンパ管 / 内皮間葉移行 / 表皮 / Raf / PI3キナーゼ / RalGEF / 皮膚表皮 / トランスジェニックマウス / MAPキナーゼ / リンパ管内皮細胞
Outline of Final Research Achievements

Recent studies have demonstrated that endothelial-to-mesenchymal transition (EndMT) is implicated in human diseases. However, the molecular basis of EndMT remains largely unknown. We found that α-smooth muscle actin-positive lymphatic endothelial cells (LECs) appear in mouse lymphedematous skin in vivo. Mouse immortalized LECs lost their characteristics and underwent EndMT when cultured in FGF2-depleted medium in vitro. FGF2 depletion acted synergistically with TGFβ to induce EndMT. In contrast, H-Ras-overexpressing LECs were resistant to EndMT. Activation of Ras not only upregulated FGF2-induced activation of the Erk MAP kinases and LEC-specific gene expression, but also suppressed TGFβ-induced activation of Smad2 by modulating Smad2 phosphorylation by Erk MAPKs. Our findings provide a new insight into the FGF2-Ras-MAPK-dependent mechanism that maintains and modulates the LEC trait.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2016 2014

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Phospholipase Cγ2 Is Required for Luminal Expansion of the Epididymal Duct during Postnatal Development in Mice.2016

    • Author(s)
      Ichise H, Ichise T, Yoshida N.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 3 Pages: e0150521-e0150521

    • DOI

      10.1371/journal.pone.0150521

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of a tamoxifen-inducible Cre-driver mouse strain for widespread and temporal genetic modification in adult mice2016

    • Author(s)
      Hirotake Ichise, Akiko Hori, Seiji Shiozawa, Saki Kondo, Yumi Kanegae, Izumu Saito, Taeko Ichise, and Nobuaki Yoshida
    • Journal Title

      Experimental Animals

      Volume: 65 Issue: 3 Pages: 231-244

    • DOI

      10.1538/expanim.15-0126

    • NAID

      130006882370

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Cd6 gene as a permissive locus for targeted transgenesis in the mouse.2014

    • Author(s)
      Ichise, H., Ichise, T., Sasanuma, H., Yoshida, N.
    • Journal Title

      genesis

      Volume: 52 Issue: 5 Pages: 440-450

    • DOI

      10.1002/dvg.22779

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] FGF2-induced Ras-MAPK signalling maintains lymphatic endothelial cell identity by upregulating endothelial-cell-specific gene expression and suppressing TGFβ signalling through Smad2.2014

    • Author(s)
      Ichise, T., Yoshida, N., Ichise, H.
    • Journal Title

      Journal of Cell Science

      Volume: 127 Pages: 845-857

    • DOI

      10.1242/jcs.137836

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

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Published: 2014-07-25   Modified: 2019-07-29  

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