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Maintenance mechanism of anterior pituitary progenitor cells by homophilic cell adhesion

Research Project

Project/Area Number 25460275
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionJichi Medical University

Principal Investigator

Kikuchi Motoshi  自治医科大学, 医学部, 教授 (60332988)

Co-Investigator(Kenkyū-buntansha) AZUMA Morio  自治医科大学, 医学部, 助教 (90709643)
YASHIRO Takashi  自治医科大学, 医学部, 教授 (80119859)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords下垂体前葉 / 細胞分化 / カドヘリン / Notch / 下垂体 / cadherin / 前駆細胞 / 腺下垂体 / 細胞接着 / 細胞間相互作用 / 細胞接着因子 / Notchシグナル
Outline of Final Research Achievements

The anterior pituitary is composed of types of hormone-producing cells. Progenitor cells that can differentiate into the types of cells were reported to be maintained in adult tissue. Differentiation is regulated by humoral factors, however, the mechanism to maintain progenitor cells was not understood. We previously showed Notch signaling, a regulator of progenitor cells in many organs, is activated in particular cell aggregates in rat pituitary. In this study, immunohistochemistry revealed a close relationship between Notch signaling molecules and cell adhesion molecule E-cadherin. Expression of the target HES1 decreased by E-cadherin inhibition in primary culture. Notch receptor and ligand were always co-localized within cells, suggesting homologous cells have reciprocal effects. We are further analyzing genes regulated by Notch signaling. The present study suggests that E-cadherin-mediated cell attachment is responsible for maintenance of progenitor cells through Notch signaling.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (22 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 5 results) Presentation (11 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Folliculostellate cell interacts with pericyte via TGFbeta2 in rat anterior pituitary.2016

    • Author(s)
      Tsukada T, Azuma M, Horiguchi K, Fujiwara K, Kouki T, Kikuchi M, Yashiro T.
    • Journal Title

      J Endocrinol

      Volume: JOE-16-0033

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of retinoic acid on growth hormone-releasing hormone receptor, growth hormone secretagogue receptor gene expression and growth hormone secretion in rat anterior pituitary cells.2016

    • Author(s)
      Maliza R, Fujiwara K, Tsukada T, Azuma M, Kikuchi M, Yashiro T.
    • Journal Title

      Endocr J

      Volume: Epub

    • NAID

      130005159557

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Maintenance of the Extracellular Matrix in Rat Anterior Pituitary Gland: Identification of Cells Expressing Tissue Inhibitors of Metalloproteinases2015

    • Author(s)
      Azuma M, Tofrizal A, Maliza R, Batchuluun K, Ramadhani D, Syaidah R, Tsukada T, Fujiwara K, Kikuchi M, Horiguchi K, Yashiro T
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 48 Issue: 6 Pages: 185-192

    • DOI

      10.1267/ahc.15020

    • NAID

      130005116785

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the heparin-binding growth factor midkine and its receptor, Ptprz1, in adult rat pituitary2014

    • Author(s)
      Fujiwara K, Horiguchi K, Maliza R, Tofrizal A, Batchuluun K, Ramadhani D, Syaidah R, Tsukada T, Azuma M, Kikuchi M, Yashiro T
    • Journal Title

      Cell Tissue Res

      Volume: 359 Issue: 3 Pages: 909-914

    • DOI

      10.1007/s00441-014-2073-8

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland2014

    • Author(s)
      Ramadhani D, Tsukada T, Fujiwara K, Azuma M, Kikuchi M, Yashiro T
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 47 Issue: 5 Pages: 231-237

    • DOI

      10.1267/ahc.14031

    • NAID

      130004700244

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Folliculostellate Cells Are Required for Laminin Release from Gonadotrophs in Rat Anterior Pituitary2014

    • Author(s)
      Tsukada T, Fujiwara K, Horiguchi K, Azuma M, Ramadhani D, Tofrizal A, Batchuluun K, Maliza R, Syaidah R, Kikuchi M, Yashiro T
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 47 Issue: 5 Pages: 239-245

    • DOI

      10.1267/ahc.14036

    • NAID

      130004700245

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In situ hybridization analysis of the temporospatial expression of the midkine/pleiotrophin family in rat embryonic pituitary gland2014

    • Author(s)
      Fujiwara K,他9名
    • Journal Title

      Cell Tissue Res.

      Volume: 357 Issue: 1 Pages: 337-344

    • DOI

      10.1007/s00441-014-1875-z

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reassembly of Anterior Pituitary Organization by Hanging Drop Three-Dimensional Cell Culture2013

    • Author(s)
      Tsukada T, Kouki T, Fujiwara K, Ramadhani D, Horiguchi K, Kikuchi M, and Yashiro T
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 46 Issue: 4 Pages: 121-127

    • DOI

      10.1267/ahc.13015

    • NAID

      130003361079

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of T-cadherin as a novel proliferation associated protein on folliculo-stellatecellsin ratanterior pituitary2016

    • Author(s)
      Khongorzul Batchuluun et al.
    • Organizer
      第121回 日本解剖学会総会・全国学術集会
    • Place of Presentation
      福島県郡山市
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gene expression analysis of folliculostellate cells in ‘transitional zone’ of anterior pituitary gland of rat – special relevance to circadian rhythm –2015

    • Author(s)
      Rita Maliza et al.
    • Organizer
      第30回日本下垂体研究会
    • Place of Presentation
      富山県黒部市
    • Year and Date
      2015-08-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] E-cadherin mediates Notch signaling in the rat anterior pituitary2015

    • Author(s)
      Batchuluun K, Azuma M, Yashiro T, Kikuchi M
    • Organizer
      第120回 日本解剖学会総会・全国学術集会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of tissue inhibitors of metalloproteinases-expressing cells in rat anterior pituitary gland2014

    • Author(s)
      Azuma M, Batchuluun K, Fujiwara K, Tsukada T, Kikuchi M, Yashiro T
    • Organizer
      The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞接着因子カドヘリンから腺下垂体前駆細胞を考える2014

    • Author(s)
      菊地元史
    • Organizer
      第 18 回日本内分泌病理学会学術総会
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2014-11-01 – 2014-11-02
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] . Histological and functional characterization of Notch signaling in the adult rat pituitary gland2014

    • Author(s)
      Batchuluun K, Azuma M, Yashiro T, Kikuchi M
    • Organizer
      第41回日本神経内分泌学会学術集会プログラム
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Presentation] Effect of Notch2 signaling on cell proliferation and Sox2 expression in S100b-positive cells in the rat pituitary gland2014

    • Author(s)
      Batchuluun K, Azuma M, Yashiro T, Kikuchi M
    • Organizer
      第29回日本下垂体研究会学術集会
    • Place of Presentation
      東京都八王子市
    • Year and Date
      2014-08-08 – 2014-08-10
    • Related Report
      2014 Research-status Report
  • [Presentation] ラット下垂体前葉におけるエンケファリン前駆体遺伝子発現細胞の同定2014

    • Author(s)
      藤原 研、塚田 岳大、東 森生、Dini Ramadhani、Tofrizal bin Alimuddin、Rita Maliza、Khongorzul Batchuluun、菊地 元史、屋代 隆
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      栃木県下野市
    • Related Report
      2013 Research-status Report
  • [Presentation] Folliculostellate cells interach with microvessel mural cell ‘pericyte’ to maintain collagen arrangement ib rat anterior pituitary development of rat2013

    • Author(s)
      Takehiro Tukada, Ken Fujiwara, Dini Ramadhai, Tom KOUKI, Motoshi Kikuchi and Takashi Yashiro
    • Organizer
      The Endocrine Society’s 95th Annual Meeting & Expo
    • Place of Presentation
      San Francisco
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット下垂体前葉における新規細胞種の発見-Desmin-immunopositive Perivascular Cell-2013

    • Author(s)
      屋代 隆、Depicha Jindatip、Alimuddin Tofrizal、幸喜 富、東 森生、菊地 元史、堀口 幸太郎
    • Organizer
      日本下垂体研究会第28回学術集会
    • Place of Presentation
      岩手県花巻市
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット下垂体前葉におけるTGFB2の発現とその作用2013

    • Author(s)
      塚田 岳大、堀口 幸太郎、菊地 元史、屋代 隆
    • Organizer
      第38回目日本比較内分泌学会大会
    • Place of Presentation
      宮崎県宮崎市
    • Related Report
      2013 Research-status Report
  • [Remarks]

    • URL

      http://www.jichi.ac.jp/histology/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 自治医科大学解剖組織

    • URL

      http://www.jichi.ac.jp/histology/

    • Related Report
      2014 Research-status Report
  • [Remarks] 自治医科大学 解剖学講座 組織学部門 研究テーマ

    • URL

      http://www.jichi.ac.jp/histology

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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