| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Outline of Final Research Achievements |
DISC1 is a candidate gene for susceptibility to schizophrenia. We have reported that DISC1 protein is accumulated on Golgi apparatus in cultured neurons, which suggests that it is a component of transporting vesicles budding from Trans-Golgi network. However, the functional significance of DISC1 on Golgi-apparatus has been essentially unknown. Here, we report that DISC1 interacts with intracellular domain of precursor pro-Neuregulin-1 (pro-NRG1) and regulates its secretion and then propose our hypothesis that DISC1 promotes the formation and budding of vesicles including DISC1-interacting proteins. For the more strict quantification of secretion, we developed a synchronized budding system of single-passed transmembrane protein through Golgi apparatus, and revealed that the budding of pro-NRG1 and the secretion of mature NRG1 were relatively impaired in full-length DISC1 deficient hippocampal neuron.
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