Involvement of store-operated calcium entry in ischemia/reperfusion injury in the heart

• Project/Area Number: 25460287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Matsuura Hiroshi 滋賀医科大学, 医学部, 教授 (60238962)
• Co-Investigator(Kenkyū-buntansha): KOJIMA Akiko 滋賀医科大学, 医学部, 助教 (50447877) ; TOYODA Futoshi 滋賀医科大学, 医学部, 助教 (90324574)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 虚血再灌流傷害 / 容量性カルシウム流入機構 / Ca2+過負荷 / 筋小胞体 / リアノジン受容体 / 2-APB / フレカイニド / ランゲンドルフ灌漑 / 心肥大モデルマウス / TAC手術 / 心室筋細胞 / Ca2+/カルモジュリン依存性タンパクキナーゼ / 筋小胞体リアンジン受容体 / カルシウムリーク / C57BL/6Jマウス / 大動脈狭窄 / 細胞拘縮 / 筋小胞体リアノジン受容体 / RyR2 / 容量性（ストア調節性）Ca2+流入機構 / SOCE / マウス摘出灌流心 / 左心室機能 / LaCl3 / TRPC

Outline of Final Research Achievements

The present investigation provides experimental evidence to support that the store-operated Ca2+ entry (SOCE) is involved in ischemia/reperfusion injury in the heart. For example, in Langendorff-perfused mouse heart model, reperfusion following 30 min of global ischemia was associated with contractile dysfunction such as elevation of left ventricular end-diastolic pressure and reduction of left ventricular developed pressure. These dysfunctions were partially but significantly attenuated by the SOCE blockers 2-aminoethoxydiphenyl borate (2-APB) and LaCl3. In addition, flecainide, which blocks Ca2+ leak through type2 ryanodine receptor and thereby preserves Ca+ content in SR, was also effective in preventing the contractile dysfunction associated with ischemia/reperfusion injury in the heart. These observations suggest that the drugs that block Ca2+ leak from SR have protective effects against ischemia/reperfusion injury in the heart.

Research Products

• [Journal Article] Lidocaine induces ROCK-dependent membrane blebbing and subsequent cell death in rabbit articular chondrocytes. (2016)
  • Author(s): Maeda T, Toyoda F, Imai S, Tanigawa H, Kumagai K, Matsuura H, Matsusue Y.
  • Journal Title: J Orthop Res Volume: 34 Issue: 5 Pages: 754-762
  • DOI: 10.1002/jor.23092
  • Peer Reviewed
• [Journal Article] Serum albumin attenuates the open-channel blocking effects of propofol on the human Kv1.5 channel (2016)
  • Author(s): Kojima A, Bai JY, Ito Y, Ding WG, Kitagawa H, Matsuura H.
  • Journal Title: Eur J Pharmacol. Volume: in press Pages: 117-126
  • DOI: 10.1016/j.ejphar.2016.04.058
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Interaction of propofol with voltage-gated human Kv1.5 channel through specific amino acids within the pore region. (2015)
  • Author(s): Kojima A, Ito Y, Ding WG, Kitagawa H, Matsuura H.
  • Journal Title: Eur J Pharmacol Volume: 764 Pages: 622-632
  • DOI: 10.1016/j.ejphar.2015.08.007
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Putative binding sites for arachidonic acid on the human cardiac Kv 1.5 channel. (2015)
  • Author(s): Bai JY, Ding WG, Kojima A, Seto T, Matsuura H.
  • Journal Title: Br J Pharmacol Volume: 172 Issue: 22 Pages: 5281-5292
  • DOI: 10.1111/bph.13314
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Phosphatidylinositol4-phosphate 5-kinase prevents the decrease in the HERG potassium current induced by Gq protein-coupled receptor stimulation (2015)
  • Author(s): Kubo T, Ding, W-G, Toyoda F, Fujii Y, Omatsu-Kabe M, Matsuura H
  • Journal Title: J Pharmacol Sci Volume: 127 Issue: 1 Pages: 127-134
  • DOI: 10.1016/j.jphs.2014.11.013
  • Peer Reviewed / Open Access
• [Journal Article] Ionic mechanisms underlying the negative chronotropic action of propofol on sinoatrial node automaticity in guinea pig heart. (2015)
  • Author(s): Kojima A, Ito Y, Kitagawa H, Matsuura H.
  • Journal Title: Br J Pharmacol Volume: 172(3) Issue: 3 Pages: 799-814
  • DOI: 10.1111/bph.12936
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gain-of-function KCNH2 mutations in patients with Brugada syndrome. (2014)
  • Author(s): Wang Q, Ohno S, Ding WG, Fukuyama M, Miyamoto A, Itoh H, Makiyama T, Wu J, Bai J, Hasegawa K, Shinohara T, Takahashi N, Shimizu A, Matsuura H, Horie M.
  • Journal Title: J Cardiovasc Electrophysiol. Volume: 25 Issue: 5 Pages: 522533-522533
  • DOI: 10.1111/jce.12361
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A novel HCN4 mutation, G1097W, is associated with atrioventricular block. (2014)
  • Author(s): Zhou J, Ding WG, Makiyama T, Miyamoto A, Matsumoto Y, Kimura H, Tarutani Y, Zhao J, Wu J, Zang WJ, Matsuura H, Horie M.
  • Journal Title: Circ J Volume: 78(4) Pages: 2014-2014
  • NAID: 130003391031
  • Peer Reviewed / Open Access
• [Journal Article] Direct negative chronotropic action of desflurane on sinoatrial node pacemaker activity in the guinea pig heart (2014)
  • Author(s): Kojima A, Ito Y, Kitagawa H, Matsuura H, Nosaka S
  • Journal Title: Anesthesiology Volume: 120 Issue: 6 Pages: 1400-1413
  • DOI: 10.1097/aln.0000000000000165
  • Peer Reviewed / Open Access
• [Journal Article] Long QT Syndrome Type8: Novel CACNA1C Mutations Causing QT Prolongation and Variant Phenotypes. (2014)
  • Author(s): Fukuyama M, Wang Q, Kato K, Ohno S, Ding WG, Toyoda F, Itoh H, Kimura H, Makiyama T, Ito M, Matsuura H, Horie M.
  • Journal Title: Europace. Volume: in press Issue: 12 Pages: 1828-37
  • DOI: 10.1093/europace/euu063
  • Peer Reviewed / Open Access
• [Journal Article] Novel intracellular mediator of adiponectin secretion from adipocyte (2014)
  • Author(s): Omatsu-Kanbe M, Matsuura H
  • Journal Title: J Physiol Volume: 592 Issue: 23 Pages: 5141-5141
  • DOI: 10.1113/jphysiol.2014.284778
  • Peer Reviewed
• [Journal Article] Prion protein- and cardiac troponin T-marked interstitial cells from the adult myocardium spontaneously develop into beating cardiomyocytes (2014)
  • Author(s): Omatsu-Kanbe M, Nishino Y, Nozuchi N, Sugihara, H, Matsuura, Matsuura H
  • Journal Title: Scientific Reports Volume: 4:7301 Issue: 1 Pages: 1-11
  • DOI: 10.1038/srep07301
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ca2+/calmodulin potentiates IKs in sinoatrial node cells by activating Ca2+/calmodulin-dependent protein kinase II. (2014)
  • Author(s): Xie Y, Ding WG, Matsuura H.
  • Journal Title: Pflugers Arch Volume: in press Issue: 2 Pages: 241-51
  • DOI: 10.1007/s00424-014-1507-1
  • Peer Reviewed
• [Journal Article] A novel KCNQ1 missense mutation identified in a patient with juvenile-onset atrial fibrillation causes constitutively open IKs channels. (2014)
  • Author(s): Hasegawa K, Ohno S, Ashihara T, Itoh H, Ding WG, Toyoda F, Makiyama T, Aoki H, Nakamura Y, Delisle BP, Matsuura H, Horie M.
  • Journal Title: Heart Rhythm Volume: 11 Issue: 1 Pages: 67-75
  • DOI: 10.1016/j.hrthm.2013.09.073
  • Peer Reviewed
• [Journal Article] Cardiac channelopathies associated with infantile fatal ventricular arrhythmias: from the cradle to the bench. (2014)
  • Author(s): Kato K, Makiyama T, Wu J, Ding WG, Kimura H, Naiki N, Ohno S, Itoh H, Nakanishi T, Matsuura H, Horie M.
  • Journal Title: J Cardiovasc Electrophysiol Volume: 25 Issue: 1 Pages: 66-73
  • DOI: 10.1111/jce.12270
  • Peer Reviewed
• [Journal Article] A Molecular Mechanism for Adrenergic-Induced Long QT Syndrome. (2014)
  • Author(s): Wu J, Naiki N, Ding WG, Ohno S, Kato K, Zang WJ, Delisle BP, Matsuura H, Horie M.
  • Journal Title: J Am Coll Cardiol Volume: 63 Issue: 8 Pages: 819827-819827
  • DOI: 10.1016/j.jacc.2013.08.1648
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Improved functional expression of human cardiac kv1.5 channels and trafficking-defective mutants by low temperature treatment. (2014)
  • Author(s): Ding WG, Xie Y, Toyoda F, Matsuura H.
  • Journal Title: PLoS One Volume: 9 Issue: 3 Pages: e92923-e92923
  • DOI: 10.1371/journal.pone.0092923
  • Peer Reviewed
• [Journal Article] A rare KCNE1 polymorphism, D85N, as a genetic modifier of long QT syndrome (2014)
  • Author(s): Hasegawa K, Ohno S, Itoh H, Makiyama T, Aiba T, Nakano Y, Shimizu W, Matsuura H, Makita N, Horie M
  • Journal Title: J Arrhythmia Volume: in press Issue: 3 Pages: 161-166
  • DOI: 10.1016/j.joa.2013.08.004
  • Peer Reviewed
• [Journal Article] Irbesartan-mediated AT1 receptor blockade attenuates hyposmostic-induced enhancement of IKs current and prevents shortening of action potential duration in atrial myocytes. (2013)
  • Author(s): Wu J, Ding W-G, Zhao J, Zang W-J, Matsuura H, Horie M
  • Journal Title: J Renin Angiotensin Aldosterone Syst Volume: in press Issue: 4 Pages: 1-7
  • DOI: 10.1177/1470320312474855
  • Peer Reviewed
• [Journal Article] Sevoflurane protects ventricular myocytes against oxidative stress-induced cellular Ca2+ overload and hypercontracture. (2013)
  • Author(s): Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
  • Journal Title: Anesthesiology Volume: 119 Issue: 3 Pages: 606-620
  • DOI: 10.1097/aln.0b013e318292ee52
  • Peer Reviewed
• [Journal Article] Remifentanil has minimal direct effect on sinoatrial node pacemaker activity in guinea-pig heart. (2013)
  • Author(s): Kojima A, Ito Y, Kitagawa H, Matsuura H, Nosaka S.
  • Journal Title: Anesth Analg Volume: 117 Issue: 5 Pages: 1072-1077
  • DOI: 10.1213/ane.0b013e3182a470ec
  • Peer Reviewed
• [Journal Article] The COX-2 selective blocker etodolac inhibits TNFα-induced apoptosis in isolated rabbit articular chondrocytes. (2013)
  • Author(s): Kumagai K, Kubo M, Imai S, Toyoda F, Maeda T, Okumura N, Matsuura H, Matsusue Y.
  • Journal Title: International Journal of Molecular Science Volume: 14 Issue: 10 Pages: 19705-15
  • DOI: 10.3390/ijms141019705
  • Peer Reviewed
• [Journal Article] Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells (2013)
  • Author(s): Shigeki Kiyonaka, Taketoshi Kajimoto, Reiko Sakaguchi, Daisuke Shinmi, Mariko Omatsu-Kanbe, Hiroshi Matsuura, Hiromi Imamura, Takenao Yoshizaki, Itaru Hamachi, Takashi Morii, Yasuo Mori
  • Journal Title: Nature Methods Volume: 10 Issue: 12 Pages: 1232-1238
  • DOI: 10.1038/nmeth.2690
  • Peer Reviewed
• [Journal Article] Ischemic survival and constitutively active autophagy in self-beating atypically-shaped cardiomyocytes (ACMs): Characterization of a new subpopulation of heart cells (2013)
  • Author(s): Omatsu-Kanbe M, Matsuura H
  • Journal Title: J Physiol Sci Volume: 63 Issue: 1 Pages: 17-29
  • DOI: 10.1007/s12576-012-0236-5
  • NAID: 10031169584
  • Peer Reviewed
• [Journal Article] セボフルランの心筋イオンチャネルの制御機構：1．洞房結節細胞における陰性変時作用／2．心室筋細胞におけるストア作動性Ca2+流入機構の抑制 (2013)
  • Author(s): 小嶋 亜希子，北川 裕利，松浦 博，野坂 修一
  • Journal Title: 麻酔 Volume: 62
  • Peer Reviewed
• [Presentation] ヒト梗塞心筋組織におけるプリオンタンパク質を発現する心筋前駆細胞の局在 (2016)
  • Author(s): 尾松 万里子、西野 裕香、野土 希実、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] 心臓ペースメーカー細胞の緩徐脱分極相におけるジヒドロピリジン感受性ナトリウム電流の寄与 (2016)
  • Author(s): 豊田 太、丁 維光、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] 細胞外ATPはP2X受容体チャネルを活性化して洞房結節細胞の自動性を抑制する (2016)
  • Author(s): 上田 梨加、狹川 浩規、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] マウス心房筋HL-1細胞におけるPitx2cの過剰発現がカリウムチャネルの発現パターンに及ぼ す影響 (2016)
  • Author(s): 小野 真由子、豊田 太、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] ヒト心筋Kv1.5チャネルに及ぼすプロテインキナーゼ Cの調節作用 (2016)
  • Author(s): 丁 維光、白 佳玉、豊田 太、ベリエ ジャンピエル、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] 心臓L型Ca 2+ チャネルのベラパミル感受性の生後変化 (2016)
  • Author(s): 狹川 浩規、上田 梨加、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] ヒトKv1.5チャネルと抗不整脈薬ベプリジルとの相互作用について (2016)
  • Author(s): 松原 一樹、丁 維光、白 佳玉、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] ウサギ軟骨細胞におけるP2X7受容体の機能的発現 (2016)
  • Author(s): 谷川 仁士、豊田 太、前田 勉、熊谷 康佑、今井 晋二、松浦 博
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] Cardiac progenitor cells that spontaneously develop into beating cardiomyocytes in the adult mouse heart. (2015)
  • Author(s): Nishino, Y., Omatsu-Kanbe, M., Nozuchi, N., Matsuura, H.
  • Organizer: 8th FAOPS Congress; Federation of the Asian and Oceanian Physiological Societies Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Human cardiac progenitor cells expressing prion protein and cardiac troponin T in the normal and infarcted heart. (2015)
  • Author(s): Nozuchi, N., Omatsu-Kanbe, M., Nishino, Y., Matsuura, H.
  • Organizer: 8th FAOPS Congress; Federation of the Asian and Oceanian Physiological Societies Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] モルモット心筋の電気活動に及ぼすβアドレナリン受容体の調節作用 (2015)
  • Author(s): 丁 維光、白 佳玉、謝 宇、豊田 太、Bellier Jeanpierre、松浦 博
  • Organizer: 第120回日本解剖学会総会・全国学術集会、第92回日本生理学会大会・合同大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] リドカインはウサギ軟骨細胞にROCK依存性の膜ブレブと細胞死を起こす (2015)
  • Author(s): 前田 勉、豊田 太、今井 晋二、谷川 仁士、熊谷 康佑、松浦 博、松末 吉隆
  • Organizer: 第120回日本解剖学会総会・全国学術集会、第92回日本生理学会大会・合同大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 拍動する細胞 atypically-shaped cardiomyocytes (ACMs) の心筋組織における起源：プリオンタンパク質と心筋型トロポニンTを共発現する間隙細胞 (2015)
  • Author(s): 尾松万里子、野土 希実、西野 裕香、松浦 博
  • Organizer: 第120回日本解剖学会総会・全国学術集会、第92回日本生理学会大会・合同大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 心臓ペースメーカー細胞におけるL型Ca2+チャネル（Cav1.3）と持続性内向きNa+電流の機能相関 (2015)
  • Author(s): 豊田 太、丁 維光、松浦 博
  • Organizer: 第120回日本解剖学会総会・全国学術集会、第92回日本生理学会大会・合同大会
  • Place of Presentation: 神戸国際会議場・展示場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Functional analysis on a novel KCNH2 mutation identified in a Familial short QT syndrome (2014)
  • Author(s): Wang Q, Ohno S, Ding W-G, Bai J, Makiyama T, Matsuura H, Horie M.
  • Organizer: Cardiac Electrophysiology Society Meeting
  • Place of Presentation: シカゴ／アメリカ合衆国イリノイ州
  • Year and Date: 2014-11-15
• [Presentation] ヒト心筋Kv1.5電流に及ぼすアラキドン酸の作用機構 (2014)
  • Author(s): 白 佳玉，丁 維光，謝 宇，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] 洞房結節細胞のIKsに及ぼすβ2受容体刺激の調節作用 (2014)
  • Author(s): 丁 維光，白 佳玉，謝 宇，豊田 太，ベリエ ジャンピエル，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] 心臓洞房結節細胞のIKs電流に対するH2O2の作用機序 (2014)
  • Author(s): 謝 宇，丁 維光，白 佳玉，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] マウス洞房結節細胞におけるCav1.3と持続性内向きNa+電流の関連 (2014)
  • Author(s): 豊田 太，Mesirca Pietro，Dubel Stefan，丁 維光，Striessnig Joerg，Mangoni E Matteo，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] マウス心室組織より同定された自動性を有するatypically-shaped cardiomyocytes (ACMs) の起源：Prion protein (PrP) をマーカーとする心筋前駆細胞の発見 (2014)
  • Author(s): 西野 裕香，野土 希実，尾松 万里子，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] ヒト心室組織におけるprion protein (PrP) とcardiac troponin T (cTnT) を共発現する心筋前駆細胞の発見 (2014)
  • Author(s): 野土 希実，西野 裕香，尾松 万里子，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] GTP cyclohydrolase 1 and its regulatory protein (GFRP) in the rat brain stem (2014)
  • Author(s): Bellier Jean-Pierre, Ding Wei-Guang, Sakaue Yuko, Yasuhara Osamu, Matsuura Hiroshi, Tooyama Ikuo
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Presentation] Prion protein (PrP) 陽性心筋前駆細胞の融合による新たな心筋細胞の形成：マウス心室組織より同定されたatypically-shaped cardiomyocytes (ACMs) の特徴 (2014)
  • Author(s): 尾松 万里子，松浦 博
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス
• [Book] AUTOPHAGY: CANCER, OTHER PATHOLOGIES, INFLAMMATION, IMMUNITY, INFECTION, AND AGING (2015)
  • Author(s): Mariko Omatsu-Kanbe, Hiroshi Matsuura
  • Publisher: ACADEMIC PRESS
• [Remarks] 滋賀医科大学 研究情報データベース 研究者総覧・研究業績一覧
  • URL: http://www.shiga-med.ac.jp/db/pub_top.php
• [Remarks] 滋賀医科大学 研究情報データベース 研究者総覧・研究業績一覧
  • URL: http://www.shiga-med.ac.jp/db/pub_top.php