| Project/Area Number |
25460290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KISHI Hiroko 山口大学, 医学(系)研究科(研究院), 准教授 (40359899)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIYA Katsuko 鹿児島大学, 農学部, 講師 (00379942)
KOBAYASHI Sei 山口大学, 大学院医学系研究科, 教授 (80225515)
ZHANG Ying 山口大学, 大学院医学系研究科, 助教 (10711260)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 血管平滑筋 / 血管異常収縮 / 細胞骨格関連分子 / 細胞運動・遊走 / 病的シグナル伝達系 / 腫瘍細胞浸潤・転移 |
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| Outline of Final Research Achievements |
The results of this research are summarized as follows: 1) We determined the site of P1, a cytoskeleton-related molecule, where interacts with Fyn, a signaling molecule which mediates abnormal contraction of vascular smooth muscle. 2) We found that V1, a cytoskeleton-related molecule, was tyrosine-dephosphorylated and cleaved at the N-terminus by the stimulation of SPC. 3) We found that the knockdown of P1 inhibited the stress-fiber formation and cell migration. 4) We found that P1 has multiple phosphorylation sites and the time course of phosphorylation was different in each site. 5) We generated smooth muscle-specific P1 knockout mice.
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