| Project/Area Number |
25460295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Tohse Noritsugu 札幌医科大学, 医学部, 教授 (80192657)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | rat / heart primordium / E-C coupling / calcium transient / α-cardiac actin / troponin-C |
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| Outline of Final Research Achievements |
During the usual excitation-contraction coupling of mature cardiomyocyte, the calcium transient brings the contraction. However, the calcium transient of immature cardiomyocyte fails to bring the contraction, especially just after the beginning of the calcium transient. No previous study shows the mechanism about the inconsistency between the beginning of the calcium transient and the beginning of the contraction. The heart primordium at the beginning of the calcium transient is so small that it has been considered difficult to evaluate the RNAs and proteins of cardiomyocyte. Here, we show that microarray analysis, highly sensitive western blot analysis and whole mount immunostaining are powerful techniques for evaluating the developmental change in expression amount, phosphorylation ratio and cellular localization of RNAs and proteins.
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