Molecular mechanisms of cancer metastasis induced by disrupting the WNK kinase mediated cytosolic Cl- homeostasis
| Project/Area Number |
25460297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Miyazaki Hiroaki 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (30360027)
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| Co-Investigator(Kenkyū-buntansha) |
MARUNAKA Yoshinori 京都府立医科大学, 大学院医学研究科, 教授 (00127036)
NIISATO Naomi 京都学園大学, 健康医療学部, 教授 (00237645)
HOSOGI Shigekuni 京都府立医科大学, 大学院医学研究科, 講師 (30433254)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 細胞内Cl-濃度 / 細胞増殖 / 細胞接着 / 細胞運動 / WNKキナーゼ / 細胞接着因子 / NKCC / KCC / Cl-チャネル |
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| Outline of Final Research Achievements |
A malignant tumor is a serious health problem because cancer cells can spread to distant parts of the body, so-called metastasis. Since metastasis is the most common cause of death from cancer, this process is an important therapeutic target. However, the molecular mechanisms of the metastasis are not fully understood. Our recent studies show that the cytosolic Cl- plays important roles in fundamental cellular functions. Thus, we investigated the effect of cytosolic Cl- concentration ([Cl-]c) on the cell proliferation, the cell migration and the cell invasion in several gastrointestinal tumor cell lines. Our study indicates that cytosolic Cl- is a key factor regulating expression of cell adhesion molecules, including E-cadherin and integrins and that cytosolic Cl- regulates WNK and Src kinase signal cascades involved in tumor proliferation, migration and invasion. These results strongly suggest that changes of [Cl-]c would play important roles in cancer development and progression.
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Report
(4 results)
Research Products
(21 results)
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[Presentation] CALHMイオンチャネルの構造・機能の解析2014
Author(s)
樽野陽幸, Zhong ming Ma, 新里直美, 宮崎裕明, 加塩麻紀子, 孫紅昕, Foskett J Kevin, 丸中良典
Organizer
膜シンポジウム2014
Place of Presentation
神戸
Year and Date
2014-11-26 – 2014-11-27
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[Presentation] CLC anion channel regulatory phosphorylation and conserved signal transduction domains2013
Author(s)
Miyazaki, H., Yamada, T., Parton, A., Morrison, R., Kim, S., Beth A.H., and Strange, K.
Organizer
Mechanobiology of Proteins and Cells
Place of Presentation
Mount Desert Island Biological Laboratory, Bar Harbor, ME, USA
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