Functional role of NO-induced Ca2+ release in skeletal muscle

• Project/Area Number: 25460303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Yamazawa Toshiko 東京慈恵会医科大学, 医学部, 講師 (00282616)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 一酸化窒素 / カルシウム / リアノジン受容体 / 骨格筋

Outline of Final Research Achievements

We found that nitric oxide (NO) -induced Ca2+ release (NICR), and that NICR is involved in NO-induced neuronal cell death. We investigated properties of the RyR1 channels carrying disease-associated mutations at the N-terminal region. HEK293 cells expressing the mutant RyR1 channels exhibited alterations in Ca2+ homeostasis, i.e., enhanced CICR and NICR sensitivity, decrease of ER Ca2+ contents, increases in resting cytoplasmic Ca2+ concentration, changes in pattern of electrostatic interaction. We investigated characterization of RyR1C3636A channels in skeletal muscle.

Research Products

• [Journal Article] Nitric oxide-induced calcium release: neuronal cell death (2016)
  • Author(s): 山澤 徳志子、柿澤 昌
  • Journal Title: Folia Pharmacologica Japonica Volume: 147 Issue: 4 Pages: 200-205
  • DOI: 10.1254/fpj.147.200
  • NAID: 130005143839
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed
• [Journal Article] Nitric-oxide induced calcium release: regulatory mechanism and physiological function (2016)
  • Author(s): 柿澤 昌、山澤徳志子
  • Journal Title: Folia Pharmacologica Japonica Volume: 147 Issue: 4 Pages: 194-199
  • DOI: 10.1254/fpj.147.194
  • NAID: 130005143841
  • ISSN: 0015-5691, 1347-8397
  • Peer Reviewed
• [Journal Article] Chlorogenic acid, a polyphenol in coffee, protects neurons against glutamate neurotoxicity (2015)
  • Author(s): Mikami, Y. and Yamazawa, T.
  • Journal Title: Life Sciences Volume: 139 Pages: 69-73
  • DOI: 10.1016/j.lfs.2015.08.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Divergent Activity Profiles of Type 1 Ryanodine Receptor Channels Carrying Malignant Hyperthermia and Central Core Disease Mutations in the Amino-Terminal Region. (2015)
  • Author(s): Murayama T, Kurebayashi N, Yamazawa T, Oyamada H, Suzuki J, Kanemaru K, Oguchi K, Iino M, Sakurai T.
  • Journal Title: PLoS One Volume: 10 Issue: 6 Pages: e0130606-e0130606
  • DOI: 10.1371/journal.pone.0130606
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 第93回日本生理学会大会 (2016)
  • Author(s): 山澤德志子、中村直俊、佐藤真理、佐藤主税
  • Organizer: 大気圧電子顕微鏡による分泌腺組織の水中観察
  • Place of Presentation: 札幌国際会議場 （北海道）
  • Year and Date: 2016-03-22
• [Presentation] 大気圧電子顕微鏡による唾液腺組織の水中観察 (2016)
  • Author(s): 山澤德志子、中村直俊、佐藤真理、佐藤主税
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2016-03-09
• [Presentation] Correlation of molecular dynamics analysis and calcium signaling in mutant ryanodine receptors (2016)
  • Author(s): Toshiko Yamazawa, Takashi Murayama, Hideto Oyamada, Junji Suzuki, Nagomi Kurebayashi, Kazunori Kanemaru, Maki Yamaguch, Shigeru Takemori, Chikara Sato, Masamitsu Iino
  • Organizer: 60 th The Biophysical Society Annual Meeting
  • Place of Presentation: Los Angeles CA, USA
  • Year and Date: 2016-02-27
  • Int'l Joint Research
• [Presentation] 変異リアノジン受容体における分子動力学解析とカルシウムシグナル可視化解析の相関 (2015)
  • Author(s): 山澤 德志子, 村山 尚, 小山田 英人, 鈴木 純二, 呉林 なごみ, 金丸 和典, 山口 眞紀, 竹森 重, 小口 勝司, 櫻井 隆, 飯野 正光
  • Organizer: 第53回生物物理学会年会
  • Place of Presentation: 金沢大学（石川県）
  • Year and Date: 2015-09-13
• [Presentation] 骨格筋の増殖・分化に及ぼすポリアミンの作用 (2015)
  • Author(s): 山澤 德志子、大野 哲生、村山 尚、大城戸 真喜子、櫻井 隆、山口 眞紀
  • Organizer: 第一回日本筋学会学術集会
  • Place of Presentation: 国立精神・神経センター （東京都）
  • Year and Date: 2015-08-08
• [Presentation] Functional analysis of type 1 ryanodine receptor carrying disease associated mutations. (2015)
  • Author(s): Toshiko Yamazawa, Takashi Murayama, Hideto Oyamada, Junji Suzuki, Nagomi Kurebayashi, Kazunori Kanemaru, Maki Yamaguchi, Shigeru Takemori, Masamitsu Iino.
  • Organizer: "Muscle: Excitation-Contraction Coupling" Gordon Research Conference
  • Place of Presentation: Sunday River ME, USA
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] ポリフェノールの神経保護作用 (2015)
  • Author(s): 山澤德志子、三上義礼
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 中枢神経における一酸化窒素によるカルシウム放出 (2015)
  • Author(s): 山澤德志子
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] 1型リアノジン受容体N末領域における悪性高熱症に関わる機能的変異 (2014)
  • Author(s): 山澤 德志子、村山 尚、小山田 英人、鈴木 純二、呉林 なごみ、金丸 和典、山口 眞紀、竹森 重、飯野 正光
  • Organizer: 第52回日本生物物理学会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Functional analysis of ryanodine receptor carrying malignant hyperthermia associated mutations. (2014)
  • Author(s): Toshiko Yamazawa, Takashi Murayama, Hideto Oyamada, Junji Suzuki, kazunori Kanemaru, Nagomi Kurebayashi, Katsuji Oguch, Takashi Sakurai, Shigeru Takemori, Masamitsu Iino
  • Organizer: WCP（国際薬理学会）
  • Place of Presentation: Cape town, South Africa
  • Year and Date: 2014-07-13 – 2014-07-18
• [Presentation] Exploration of functional mutations of ryanodine receptor in malignant hyperthermia.
  • Author(s): Yamazawa T, Oyamada H, Murayama T, Kurebayashi N, Oguchi K, Sakurai T, Iino M, Takemori S.
  • Organizer: IUPS(国際生理学会)
  • Place of Presentation: Birmingham, UK.
• [Presentation] 悪性高熱症関連変異をもたらしている1型リアノジン受容体の機能解析
  • Author(s): 山澤德志子、村山尚、小山田英人、 鈴木純二、金丸和典、呉林なごみ、飯野正光、竹森重
  • Organizer: 第58回生物物理学会年会
  • Place of Presentation: 京都国際会館、京都府
• [Presentation] Functional analysis of ryanodine receptor carrying malignant hyperthermia associated mutations.
  • Author(s): Yamazawa T, Murayama T, Oyamada H, Suzuki J, Kanemaru K, Kurebayashi N, Oguch K, Sakurai T, Takemori S, Iino M.
  • Organizer: 58th Biophysical society annual meeting
  • Place of Presentation: SanFrancisco, USA
• [Presentation] 骨格筋の増殖・分化に対するポリアミンの作用
  • Author(s): 山澤徳志子、大野哲夫、大城戸真喜子、山本裕大、山口眞紀、竹森重
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学、鹿児島県
• [Presentation] N末端悪性高熱症関連突然変異をもたらしている1型リアノジン受容体の機能解析
  • Author(s): 山澤 德志子, 村山 尚, 小山田 英人, 鈴木 純二,金丸 和典, 呉林 なごみ, 小口 勝司, 櫻井 隆 , 竹森 重 , 飯野 正光
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 東北大学、宮城県