| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We found that nitric oxide (NO) -induced Ca2+ release (NICR), and that NICR is involved in NO-induced neuronal cell death. We investigated properties of the RyR1 channels carrying disease-associated mutations at the N-terminal region. HEK293 cells expressing the mutant RyR1 channels exhibited alterations in Ca2+ homeostasis, i.e., enhanced CICR and NICR sensitivity, decrease of ER Ca2+ contents, increases in resting cytoplasmic Ca2+ concentration, changes in pattern of electrostatic interaction. We investigated characterization of RyR1C3636A channels in skeletal muscle.
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