| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Estrogen is an essential steroid hormone for ovulatory induction. Regulatory mechanisms underlying ovulatory induction were analyzed using estrogen deficient (ArKO) mice. Responsiveness of ArKO ovaries to follicle stimulating hormone was significantly lower than that of wild-type (WT) ovaries. This was, in part, attributable to high amounts of cAMP content in ArKO ovaries prior to stimulation of the hormone, compared to that in the WT ovaries. The present study also demonstrated that co-administration of insulin with LH to 17beta-estradiol/FSH-primed ArKO mice significantly improve ovulatory rate than the rate observed in the mice stimulated with LH alone. These results indicate that estrogen keeps cAMP content at a low level before FSH stimulation and the sensitivity to insulin to be high in order to response properly to ovulatory stimulations.
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