Regulatory mechanisms of ovulation by estrogen

• Project/Area Number: 25460315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Kochi University
• Principal Investigator: Toda Katsumi 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (40197893)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エストロゲン / 遺伝子改変マウス / アロマターゼ / 排卵 / 排卵誘導 / インスリン / タンパク質リン酸化 / 生殖腺刺激ホルモン

Outline of Final Research Achievements

Estrogen is an essential steroid hormone for ovulatory induction. Regulatory mechanisms underlying ovulatory induction were analyzed using estrogen deficient (ArKO) mice. Responsiveness of ArKO ovaries to follicle stimulating hormone was significantly lower than that of wild-type (WT) ovaries. This was, in part, attributable to high amounts of cAMP content in ArKO ovaries prior to stimulation of the hormone, compared to that in the WT ovaries. The present study also demonstrated that co-administration of insulin with LH to 17beta-estradiol/FSH-primed ArKO mice significantly improve ovulatory rate than the rate observed in the mice stimulated with LH alone. These results indicate that estrogen keeps cAMP content at a low level before FSH stimulation and the sensitivity to insulin to be high in order to response properly to ovulatory stimulations.

Research Products

• [Journal Article] PNPLA3 is involved in hepatic fatty acid and triglyceride metabolism through XBP1 and modulation of endoplasmic reticulum stress in mice. (2016)
  • Author(s): Ochi T, Munekage K, Ono M, Higuchi T, Tsuda M, Hayashi Y, Okamoto N, Toda K, Sakamoto S, Oben JA, Saibara T
  • Journal Title: Hepatol Res Volume: 印刷中 Issue: 6 Pages: 584-592
  • DOI: 10.1111/hepr.12587
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of ovarian responses to equine chorionic gonadotropin of aromatase-deficient mice with or without 17-estradiol supplementation. (2016)
  • Author(s): Katsumi Toda, Yoshihiro Hayashi, Masafumi Ono, Toshiji Saibara
  • Journal Title: Endocrinology Volume: 157 Issue: 5 Pages: 2093-2103
  • DOI: 10.1210/en.2015-1701
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Co-administration of insulin with a gonadotropin partly improves ovulatory responses of estrogen-deficient mice (2015)
  • Author(s): Katsumi Toda, Yoshihiro Hayashi, Masafumi Ono, Toshiji Saibara
  • Journal Title: Molecular and Cellular Endocrinology Volume: 411 Pages: 177-186
  • DOI: 10.1016/j.mce.2015.04.027
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regional difference in sex steroid action on formation of morphological sex differences in the anteroventral periventricular nucleus and principal nucleus of the bed nucleus of the stria terminalis. (2014)
  • Author(s): Kanaya M, Tsuda M., Sagoshi S, Nagata K, Morimoto C, Thu CK, Today K, Kato S, Ogawa S, Tsukahara S
  • Journal Title: PLoS One Volume: 9 Issue: 11 Pages: e112616-e112616
  • DOI: 10.1371/journal.pone.0112616
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A romatese-null mice expressing enhanc ed green fluorescent protein in germ cells provide a model system to ass ess estrogen-dependent ovulatory res ponses (2013)
  • Author(s): Toda K,Ono M,Saibara T(他3名)
  • Journal Title: Transgenic Res Volume: (E-pub) Issue: 2 Pages: 293-302
  • DOI: 10.1007/s11248-013-9771-y
  • Peer Reviewed / Open Access