Regulatory mechanisms of estrogen receptor gene expression by alternative promoter usage and alternative splicing
Project/Area Number |
25460319
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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Research Institution | Nippon Medical School |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | エストロゲン / エストロゲン受容体 / 多重プロモーター / 選択的スプライシング / スプライス変異体 / 恒常的転写活性化 / 生殖生理学 / 神経内分泌学 / ホルモン感受性腫瘍 / 遺伝子発現制御 / 生殖機能形態学 |
Outline of Final Research Achievements |
To determine regulatory mechanisms of estrogen receptor gene expression, alternative promoter usage and alternative splicing profiles of the estrogen receptor genes were examined. We identified modular structures of the estrogen receptor genes and several splice variants encoding N- and C-terminally-truncated estrogen receptor proteins. Some C-terminally-truncated estrogen receptor variants exhibited constitutive transcriptional transactivation in transfected cells. Ligand-independent transcriptional transactivation by steroid hormone receptors is deduced to be one of the candidate causes of malignant development of hormone-sensitive tumors. Therefore, our results may provide key mechanistic information explaining the cause of hormone-independent cell overgrowth in estrogen-sensitive tumors.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Establishment of an in vitro cell line experimental system for the studablishment of an in vitro cell line experimental system for the study oy of inhalational anesthetic mechanisms.2016
Author(s)
Nagamoto S, Iijima N, Ishii H, Takumi K, Higo S, Aikawa S, Anzai M, Matsuo I, Nakagawa S, Takashima N, Shigeyoshi Y, Sakamoto A, Ozawa H.
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Journal Title
Neurosci Lett.
Volume: 4;620:
Pages: 163-168
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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