Ion channel involved in stress-response and anxiety-like behavior

• Project/Area Number: 25460323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Kurashiki University of Science and the Arts (2015-2016); Kansai Medical University (2013-2014)
• Principal Investigator: Okada Masayoshi 倉敷芸術科学大学, 生命科学部, 教授 (40334677)
• Co-Investigator(Kenkyū-buntansha): 松田 博子 関西医科大学, 医学部, 教授 (10181736) ; ROMERO GUSTAVO 関西医科大学, 医学部, 研究員 (30572820)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: K+ チャネル / TREK / うつ / 不安 / K+ channel / ストレス / TREK-1 / 不安様行動 / うつ様行動 / c-fos / パッチクランプ / イオンチャネル / 視床室傍核 / 抑制性シナプス後電流

Outline of Final Research Achievements

We have screened botanical compounds and found a novel 2-pore-domain type K+ channel activator with patch-clamp recordings. This compound showed both anxiolytic and antidepressive effects in the mouse behavior tests. The compound had no effect on anti-c-fos immunoreactivity in the brain of nontreated-mouse but suppressed a stress-induced increase in the anti-c-fos immunoreactivity in a nucleus. This site of action was different from those of current anxiolytic or antidepressant, suggesting a novel mechanism this compound.
We also revealed the involvement of intracellular transport in the run-up of TREK-1 channel current.

Research Products

• [Int'l Joint Research] ベトナム科学技術アカデミー/国立天然物化学研究所(ベトナム)
• [Journal Article] Involvement of intracellular transport in TREK-1c current run-up in 293T cells. (2017)
  • Author(s): Andharia N, Joseph A, Hayashi M, Okada M, Matsuda H
  • Journal Title: Channels (Austin) Volume: 11 Issue: 3 Pages: 1-12
  • DOI: 10.1080/19336950.2017.1279368
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A pore forming peptide from spider Lachesana sp. venom induced neuronal depolarization and pain. (2015)
  • Author(s): Okada M, Corzo G, Romero-Perez GA, Coronas F, Matsuda H, Possani LD.
  • Journal Title: Biochim Biophys Acta Volume: 1850 Issue: 4 Pages: 657-666
  • DOI: 10.1016/j.bbagen.2014.11.022
  • Peer Reviewed
• [Journal Article] Increase in the titer of lentiviral vectors expressing potassium channels by current blockade during viral vector production (2015)
  • Author(s): Masayoshi Okada, NaazAndharia and Hiroko Matsuda
  • Journal Title: BMC Neuroscience Volume: 16 Issue: 1 Pages: 30-30
  • DOI: 10.1186/s12868-015-0159-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The degradation of the inwardly rectifying potassium channel, Kir2.1, depends on the expression level: examination with fluorescent proteins. (2013)
  • Author(s): Okada M, Kano M, Matsuda H.
  • Journal Title: Brain Res Volume: 1528 Pages: 8-19
  • DOI: 10.1016/j.brainres.2013.07.008
  • Peer Reviewed
• [Presentation] Involvement of intracellular transport in TREK-1c current run-up in 293T cells (2017)
  • Author(s): Naaz Andharia, Ancy Joseph, Mikio Hayashi, Masayoshi Okada, Hiroko Matsuda
  • Organizer: Experimental Biology
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2017-04-25
  • Int'l Joint Research
• [Presentation] Involvement of intracellular transport in TREK-1 current in 293T cells (2017)
  • Author(s): アンダリア ナーズ，ジョセフ アンシー，林 美樹夫，岡田 誠剛，松田 博子
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2017-03-29
• [Presentation] Modifications of TREK-1c channel (2014)
  • Author(s): Masayoshi Okada, Hiroko Matsuda, Geraldo Corzo, Fredy Coronas, Lourival Possani
  • Organizer: Gordon Research Conference
  • Place of Presentation: Mount Holyoke College, South Hadley, MA, USA
  • Year and Date: 2014-07-06 – 2014-07-11