Simulation analysis on astrocytic K+ buffering
| Project/Area Number |
25460331
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Murakami Shingo 大阪大学, 医学(系)研究科(研究院), 助教 (40437314)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | アストロサイト / カリウム緩衝 / シミュレーション / システムバイオロジー / 内向き整流性カリウム電流 / 恒常性 / イオン輸送 / 水輸送 / 脳浮腫 / 内向き整流性カリウムチャネル / 水チャネル |
|---|
| Outline of Final Research Achievements |
In response to elevation of extracellular K+ concentration ([K+]out), astrocytes clear excessive extracellular K+ to maintain proper environment for neural activity. Here we conducted simulation analysis on the mechanisms of the astrocytic K+ clearance and swelling. Astrocyte models were constructed by incorporating into a compartment model various mechanisms. Simulated response of astrocyte models to high [K+]out revealed significant contributions of NKCC and Na,K-ATPase to the increases of the intracellular K+ and Cl- concentrations, and swelling. Moreover, we show that the KIR channel localized at synaptic cleft absorbs the excessive K+, while the K+ release through KIR channel localized at perivascular is enhanced. Further analysis of simulated drug effects shows that K+ uptake, K+ release and swelling can be modulated differently by blocking each of the ion channels and transporters.
|
Report
(4 results)
Research Products
(19 results)
-
-
-
-
-
-
[Journal Article] 内耳内リンパ液の特殊電位環境の成立機構の理解2013
Author(s)
Hiroshi Hibino, Fumiaki Nin, Shingo Murakami, Katsumi Doi, Toshihiro Suzuki, Yasuo Hisa, Yoshihisa Kurachi
-
Journal Title
Nippon Jibiinkoka Gakkai Kaiho
Volume: 116
Issue: 2
Pages: 60-68
DOI
NAID
ISSN
0030-6622, 1883-0854
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
