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Activation mechanisms of AMPK by nitrite

Research Project

Project/Area Number 25460332
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General pharmacology
Research InstitutionThe University of Tokushima

Principal Investigator

TSUCHIYA Koichiro  徳島大学, 大学院医歯薬学研究部, 教授 (70301314)

Co-Investigator(Kenkyū-buntansha) TAMAKI Toshiaki  徳島大学, 大学院医歯薬学研究部, 教授 (80179879)
IKEDA Yasumasa  徳島大学, 大学院医歯薬学研究部, 准教授 (60432754)
KIHIRA Yoshitaka  徳島大学, 大学院医歯薬学研究部, 助教 (90377276)
ISHIZAWA Keisuke  徳島大学, 大学院医歯薬学研究部, 教授 (60398013)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords亜硝酸塩 / AMPK / 糖代謝 / 脂質代謝
Outline of Final Research Achievements

We have shown that dietary supplied nitrate, which substantially exists in our daily diets including vegetables, is reduced into nitrite, and becomes a significant source for NO production playing tissues- and vaso-protective roles especially in hypoxic tissues. During the study period, we found a phenomenon that nitrite directly activated the AMP-activated protein kinase (AMPK) in a concentration dependent manner. To clarify this mechanism, we investigated the activation of AMPK by a549 cells that lacks LKB1 and fails to facilitate the AMPK phosphorylation. In addition, pre-treatment of STO609, a CaMKK inhibitor, suppressed the AMPK phosphorylation. These data suggest that nitrite activates AMPK through both LKB1 and CaMKK pathway.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 Other

All Presentation (16 results)

  • [Presentation] Dietary nitrite ameliorates glucose tolerance and hyperlipidemia in diet-induced obesity rats.2016

    • Author(s)
      Koichiro Tsuchiya, Licht Miyamoto, Yosuke Tomida, Megumi Yamane, Yuuki Tsuchihashi, Katsunori Tsuda, Yasumasa Ikeda, Toshiaki Tamaki
    • Organizer
      The 9th International NO Conference
    • Place of Presentation
      仙台国際センター・宮城県・仙台市
    • Year and Date
      2016-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] Dietary nitrite ameliorates glucose tolerance and hyperlipidemia in diet-induced obesity rats.2015

    • Author(s)
      Yuki Tsuchihashi, Licht Miyamoto, Yosuke Tomida, Megumi Yamane, Kazuya Takenokuma, Yasumasa Ikeda, Toshiaki Tamaki, Koichiro Tsuchiya
    • Organizer
      The 6th International Conference on Food Factors: ICoFF 2015
    • Place of Presentation
      Coex, Seoul, KOREA
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Impact of AMPK activation on the metabolomic alterations in contracting skeletal muscles.2015

    • Author(s)
      Licht Miyamoto, Tatsuro Egawa, Rieko Oshima, Eriko Kurogi, Koichiro Tsuchiya, Tatsuya Hayashi
    • Organizer
      The 6th International Conference on Food Factors: ICoFF 2015
    • Place of Presentation
      Coex, Seoul, KOREA
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] カフェインによるAMPK活性化メカニズムの解析2015

    • Author(s)
      竹之熊 和也、宮本 理人、友川 剛己、土屋 浩一郎
    • Organizer
      第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      高知市文化プラザカルポート・高知県・高知市
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sodium nitrite activates AMPK-eNOS signaling pathway2014

    • Author(s)
      山根 萌
    • Organizer
      2014 Asian Society for Mitochondrial Research and Medicine (ASMRM) Conference
    • Place of Presentation
      Grand Hyatt Taipei, Taipei, Taiwan
    • Year and Date
      2014-11-14 – 2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト腎糸球体内皮細胞における亜硝酸塩によるAMPK-eNOS経路の活性化2014

    • Author(s)
      山根 萌
    • Organizer
      第126回日本薬理学会近畿部会
    • Place of Presentation
      和歌山県JAビル(和歌山県和歌山市)
    • Year and Date
      2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 亜硝酸塩によるAMPK-eNOS経路活性化2014

    • Author(s)
      山根 萌
    • Organizer
      生体機能と創薬シンポジウム2014
    • Place of Presentation
      近畿大学(大阪府大阪市)
    • Year and Date
      2014-08-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Significance of AMPK-eNOS pathway in renal protective effects of nitrite.

    • Author(s)
      Koichiro Tsuchiya, Licht Miyamoto, Megumi Yamane, Mai Kono, Yosuke Tomida, Keisuke Ishizawa, Yoshitaka Kihira, Yasumasa Ikeda, Toshiaki Tamaki
    • Organizer
      5th International meeting on the role of nitrite and nitrate in physiology, pathophysiology and therapeutics
    • Place of Presentation
      Pittsburgh Univ. Pittsburgh PA (USA)
    • Related Report
      2013 Research-status Report
  • [Presentation] 亜硝酸塩によるAMPK活性化作用とその意義

    • Author(s)
      宮本理人、山根萌、河野舞、冨田洋輔、石澤啓介、玉置俊晃、土屋浩一郎
    • Organizer
      第13回日本NO学会学術集会
    • Place of Presentation
      沖縄県医師会館(沖縄県沖縄市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 亜硝酸塩による腎保護作用とその機序

    • Author(s)
      土屋浩一郎、宮本理人、石澤啓介、冨田洋輔、山根 萌、木平孝高、池田康将、玉置俊晃
    • Organizer
      第66回日本酸化ストレス学会学術集会
    • Place of Presentation
      ウィンクあいち(愛知県名古屋市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Nitrite amelioates glucose tolerance and hyperlipidemia in diet-induced obesity rats

    • Author(s)
      Yosuke Tomida, Licht Miyamoto, Megumi Yamane, Kazuya Takenokuma, Mai Kono, Keisuke Ishizawa, Toshiaki Tamaki and Koichiro Tsuchiya
    • Organizer
      第18回アディポサイエンスシンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府大阪市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 亜硝酸塩による糖脂質代謝の改善作用とそのメカニズム

    • Author(s)
      冨田洋輔、宮本理人、山根萌、河野舞、竹之熊和也、石澤啓介、玉置俊晃、土屋浩一郎
    • Organizer
      次世代を担う創薬・医療薬理シンポジウム2013
    • Place of Presentation
      熊本大学薬学部(熊本県熊本市)
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト腎糸球体内皮細胞における亜硝酸塩によるAMPK-eNOS活性化経路の検討

    • Author(s)
      山根 萌、宮本理人、冨田洋輔、石澤啓介、池田康将、木平孝高、玉置俊晃、土屋浩一郎
    • Organizer
      第25回腎とフリーラジカル研究会
    • Place of Presentation
      昭和大学(東京都品川区)
    • Related Report
      2013 Research-status Report
  • [Presentation] 亜硝酸イオンのAMPK活性化作用と糖・脂質代謝に対する効果の検討

    • Author(s)
      冨田洋輔、宮本理人、山根萌、河野舞、竹之熊和也、石澤啓介、玉置俊晃、土屋浩一郎
    • Organizer
      第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
    • Place of Presentation
      松山大学(愛媛県松山市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Nitrite amelioates glucose tolerance and hyperlipidemia in diet-induced obesity rats

    • Author(s)
      Yosuke Tomida, Licht Miyamoto, Megumi Yamane, Kazuya Takenokuma, Mai Kono, Keisuke Ishizawa, Toshiaki Tamaki and Koichiro Tsuchiya
    • Organizer
      2013 International Conference on Diabetes and Metabolism &5th Scientific Meeting of the Asian Association for the Study of Diabetes
    • Place of Presentation
      Grand Hilton Seoul Hotel (Seoul, Korea)
    • Related Report
      2013 Research-status Report
  • [Presentation] Significance of AMPK in renal protective and metabolic actions of nitrite

    • Author(s)
      Licht Miyamoto, Megumi Yamane, Yosuke Tomida, Kazuya Takenokuma, Keisuke Ishizawa, Toshiaki Tamaki, Koichiro Tsuchiya
    • Organizer
      SFRRI2014
    • Place of Presentation
      京都国際会議場(京都府京都市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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