| Project/Area Number |
25460337
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YORINAKA Gyokuchin 熊本大学, 生命科学研究部, 研究員 (50418828)
SAKAGUCHI Nobuo 熊本大学, 生命科学研究部, 教授 (70192086)
|
|---|
| Research Collaborator |
ZHANG Tianli 熊本大学, 生命科学研究部
KITAMOTO Yasunori JCHO仙台病院
WANG Xiaoping 中国幹南医科大学第一附属病院
WANG Boqun 中国幹南医科大学第一附属病院
LIU Xiaozhen 中国上海応用技術学院
JIANG Baohong 中国科学院上海薬物研究所
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | TNNI3K / 金コロイド銀増幅型検査チップ / 心筋虚血 / ゲノム薬理学 / 心疾患 / ELISA / 創薬 / 血中濃度 / 血中TNNI3K濃度 / ELISA法 / 金コロイドー銀増幅法 / 急性心筋虚血 / 心筋梗塞 / 心筋損傷診断マーカー / 創薬ゲノム薬理学 / モノクローナル抗体作成 / マウス血中TNNI3K 濃度 / ヒト血中TNNI3K 濃度 / マウス心筋梗塞モデル / 国際研究者交流 / 国際研究情報交換 / モノクローナル抗体 / 虚血性心疾患 / 新型診断薬の開発 / ELISA実験 / ゲノム創薬 |
|---|
| Outline of Final Research Achievements |
Circulating TNNI3K level was investigated using anti-TNNI3K monoclonal antibodies with ELISA and gold colloid-based TNNI3K methods to diagnose cardiac ischemia diseases including acute myocardial ischemia diseases(AMI).
Our data obtained from ELISA-method shown that circulating TNNI3K levels were significantly higher in AMI patients(p<0.01), and measurement of circulating TNNI3K concentrations is a useful diagnosing tool for AMI. Using gold colloid based-silver enhanced TNNI3K biochips, not only increased sensitivity but also enhanced specificity & shortened mesurement-time significantly(15 min)compared with ELISA method(P<0.01). The gold colloid based-silver enhanced TNNI3K biochips may be a new and good bio-diagnose tool for early diagnose of some patients suddenly onset acute myocardial ischemia diseases. In conclusion, using gold colloid-based TNNI3K biochips can significantly shorten the diagnosis period, enhance the diagnosis rate for diagnosising ischemic cardiac diseases.
|
