Biochemical analysis of clathrin heavy chain, CHC17 and CHC22
| Project/Area Number |
25460338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | クラスリン / 細胞内小胞輸送 / 核移行 / ミトコンドリア / ストレス / p53 / 飢餓 / メンブレントラフィック |
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| Outline of Final Research Achievements |
Clathrin heavy chains (CHCs) are known as essential molecules for intracellular vesicular transport. Recently, some reports revealed that these play important roles in mitosis and transfer. In this study, we showed that inhibition of mitochondrial respiration increased CHC17, one of CHC isoforms, in nuclear fraction by using biochemical analysis. However, immunohistorogical analysis did not show this phenomenon. Through these experiments, we revealed the biochemical characteristics of CHC22, another isoform of CHC. By using this, we success to separate CHC17 and CHC22.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] The CHC22 clathrin-GLUT4 transport pathway contributes to skeletal muscle regeneration2013
Author(s)
Hoshino S, Sakamoto K, Vassilopoulos S, Camus SM, Griffin CA, Esk C, Torres JA, Ohkoshi N, Ishii A, Tamaoka A, Funke BH, Kucherlapati R, Margeta M, Rando TA, Brodsky FM
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Journal Title
PLoS One
Volume: 8(10)
Issue: 10
Pages: e77787-e77787
DOI
Related Report
Peer Reviewed
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