Role of ion transporter localization and activity in myogenic responses

• Project/Area Number: 25460350
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Fukuoka University
• Principal Investigator: KITA SATOMI 福岡大学, 医学部, 准教授 (10461500)
• Co-Investigator(Kenkyū-buntansha): FUJII Makoto 福岡大学, 医学部, 助教 (30398086) ; GOTOH Yusuke 福岡大学, 医学部, 助教 (90609489)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 筋原性収縮 / イオン輸送 / リン脂質

Outline of Final Research Achievements

Myogenic tone have been known to plays important role for regulation of the peripheral artery resistance and blood stream in heart, kidney and brain, but the molecular mechanism still unclear. To claryfy the role of NCX1 expression and localization in myogenic tone, we generated smooth muscle spesific NCX1-EGFP transgenic mice. We found that myogenic responses were attenuated in PIP5K-beta knockout mice. Furthermore, gene expression level of NCX1 was decreased in PIP5K-beta knockout mice, compared with wild-type mice, suggesting that regulation of NCX1 activity by PIP2 is involved in myogenic tone.

Research Products

• [Journal Article] Nicorandil stimulates a Na+/Ca2+ exchanger by acting guanylate cyclase in guinea pig cardiac myocytes. (2016)
  • Author(s): Wei J, Watanabe Y, Takeuchi K, Yamashita K, Tashiro M, Kita S, Iwamoto T, Watanabe H, Kimura J
  • Journal Title: Pflugers Arch Volume: 468(4) Issue: 4 Pages: 693-703
  • DOI: 10.1007/s00424-015-1763-8
  • Peer Reviewed / Open Access
• [Journal Article] Na+/Ca2+ exchanger-heterozygote knockout mice display increased relaxation in gastric fundus and accelerated gastric transit in vivo. (2016)
  • Author(s): Azuma YT, Hayashi S, Nishiyama K, Kita S, Mukai K, Nakajima H, Iwamoto T, Takeuchi T
  • Journal Title: Neurogastroenterol Motil Volume: 印刷中 Issue: 6 Pages: 827-836
  • DOI: 10.1111/nmo.12779
  • Peer Reviewed
• [Journal Article] Genetic knockout and pharmacologic inhibition of NCX2 cause natriuresis and hypercalciuria. (2015)
  • Author(s): Gotoh Y, Kita S, Fujii M, Tagashira H, Horie I, Arai Y, Uchida S, Iwamoto T
  • Journal Title: Biochem Biophys Res Commun Volume: 456 (2) Issue: 2 Pages: 670-675
  • DOI: 10.1016/j.bbrc.2014.12.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phosphoinositide analysis using the HPLC system equipped with a fraction collector and the TSKgel SAX column. (2015)
  • Author(s): Kita S, Tagashira H, Gotoh Y, Fujii M, Iwamoto T
  • Journal Title: Med Bull Fukuoka Univ Volume: 42 (1) Pages: 175-181
  • NAID: 110009890548
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Na+/Ca2+ exchanger 1 transgenic mice display increased relaxation in the distal colon. (2014)
  • Author(s): Nishiyama K, Morioka A, Kita S, Nakajima H, Iwamoto T, Azuma YT, Takeuchi T
  • Journal Title: Pharmacology Volume: 94 (5-6) Issue: 5-6 Pages: 230-238
  • DOI: 10.1159/000363246
  • Peer Reviewed / Open Access
• [Journal Article] Overactive bladder mediated by accelerated Ca^<2+> influx mode of Na^+/Ca^<2+> exchanger in smooth muscle (2013)
  • Author(s): Yamamura H, Cole WC, Kita S, Hotta S, Murata H, Suzuki Y, Ohya S, Iwamoto T, Imaizumi Y
  • Journal Title: Am J Physiol Cell Physiol Volume: 305(3) Issue: 3 Pages: C299-C308
  • DOI: 10.1152/ajpcell.00065.2013
  • Peer Reviewed
• [Journal Article] Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger (2013)
  • Author(s): Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
  • Journal Title: Science Volume: 341 Issue: 6142 Pages: 168-172
  • DOI: 10.1126/science.1239002
  • Peer Reviewed
• [Journal Article] Pretreatment of donor islets with the Na(+) /Ca(2+) exchanger inhibitor improves the efficiency of islet transplantation (2013)
  • Author(s): Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y.
  • Journal Title: Am J Transplant Volume: 13 Issue: 8 Pages: 2154-2160
  • DOI: 10.1111/ajt.12306
  • Peer Reviewed
• [Journal Article] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II (2013)
  • Author(s): Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
  • Journal Title: Hypertension Volume: 62(5) Issue: 5 Pages: 872-8
  • DOI: 10.1161/hypertensionaha.113.01543
  • Peer Reviewed
• [Journal Article] Na⁺/Ca²⁺ Exchanger 1/2 Double-Heterozygote Knockout Mice Display Increased Nitric Oxide Component and Altered Colonic Motility (2013)
  • Author(s): Nishiyama K, Azuma YT, Kita S, Azuma N, Hayashi S, Nakajima H, Iwamoto T, Takeuchi T
  • Journal Title: Journal of Pharmacological Sciences Volume: 123 Issue: 3 Pages: 235-245
  • DOI: 10.1254/jphs.13114FP
  • NAID: 130003382606
  • ISSN: 1347-8613, 1347-8648
  • Peer Reviewed
• [Presentation] 遺伝子改変マウスを用いた血管NCLXの機能解析 (2016)
  • Author(s): 喜多紗斗美、田頭秀章、荒井勇二、岩本隆宏
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-09
• [Presentation] 遺伝子改変マウスを用いたミトコンドリアNa+/Ca2+交換体の心血管機能解析 (2015)
  • Author(s): 喜多紗斗美、田頭秀章、岩本隆宏
  • Organizer: 第68回日本薬理学会西南部会
  • Place of Presentation: 海峡メッセ下関（山口県下関市）
  • Year and Date: 2015-11-21
• [Presentation] 遺伝子改変マウスを用いたNCLXの心血管機能解析 (2015)
  • Author(s): 喜多紗斗美、田頭秀章、岩本隆宏
  • Organizer: 生理研研究会2015「心臓・血管系の包括的な機能統合研究」
  • Place of Presentation: 岡崎自然科学研究機構明大寺地区（愛知県岡崎市）
  • Year and Date: 2015-10-29
• [Presentation] ミトコンドリアNa+/Ca2+交換体（NCLX）の生理機能： NCLXノックアウトマウスを用いた解析 (2015)
  • Author(s): 喜多紗斗美、田頭秀章、岩本隆宏
  • Organizer: 第8回トランスポーター研究会九州部会
  • Place of Presentation: 鹿児島大学医学部鶴陵会館（鹿児島県鹿児島市）
  • Year and Date: 2015-07-18
• [Presentation] NCLXノックアウトマウスの心機能解析 (2015)
  • Author(s): 喜多紗斗美、田頭秀章、岩本隆宏
  • Organizer: 第10回トランスポーター研究会年会
  • Place of Presentation: 慶應義塾大学薬学部芝共立キャンパス（東京都港区）
  • Year and Date: 2015-06-20
• [Presentation] NCXの構造・機能解析における最近の進歩 (2015)
  • Author(s): 喜多紗斗美、岩本隆宏
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] Na+/Ca2+交換輸送体の活性制御と血管トーヌス調節 (2014)
  • Author(s): 喜多紗斗美、岩本隆宏
  • Organizer: 第56回日本平滑筋学会総会
  • Place of Presentation: 新横浜プリンスホテル（神奈川県横浜市）
  • Year and Date: 2014-08-07 – 2014-08-08
• [Presentation] NCX1 inhibitor improves the efficiency of islet transplantation. (2014)
  • Author(s): 喜多 紗斗美、米良 利之、伊東 威、小玉 正太、安波 洋一、岩本 隆宏
  • Organizer: 第9回トランスポーター研究会
  • Place of Presentation: 名古屋市立大学大学院薬学研究科（愛知県名古屋市）
  • Year and Date: 2014-06-14 – 2014-06-15
• [Presentation] 膵β細胞のCa2+調節機構におけるNa+/Ca2+交換体の役割 (2014)
  • Author(s): 喜多紗斗美、岩本隆宏
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
• [Presentation] Na+/Ca2+ exchange inhibitor improves the efficiency of islet transplantation (2014)
  • Author(s): Satomi Kita, Toshiyuki Mera, Takeshi Itoh, Shohta Kodama, Yohichi Yasunami, Takahiro Iwamoto
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 東北大学百周年記念会館川内萩ホール（宮城県仙台市）
• [Presentation] マウス腸間膜細動脈の筋原性収縮およびalpha1アゴニスト収縮におけるTRPC3/6の関与について (2013)
  • Author(s): 喜多紗斗美、荒井勇二、堀江一郎、岩本隆宏
  • Organizer: 第8回トランスポーター研究会年会
  • Place of Presentation: 熊本大学薬学部（熊本県熊本市）
• [Presentation] Involvement of TRPC3/6 channels in myogenic tone regulation and alpha1-adrenoceptor-mediated vasoconstriction of murine mesenteric arteries (2013)
  • Author(s): Satomi Kita, Yuji Arai, Ichiro Horie, Takahiro Iwamoto
  • Organizer: The 2nd HD Physiology International Symposium
  • Place of Presentation: 丸の内マイプラザホール（東京都千代田区）
• [Presentation] PIP2によるNa+/Ca2+交換輸送体機能制御の分子機構と病態生理学的意義 (2013)
  • Author(s): 喜多紗斗美、岩本隆宏
  • Organizer: 第55回日本平滑筋学会総会
  • Place of Presentation: 旭川市大雪クリスタルホール（北海道旭川市）
• [Remarks] 福岡大学研究者情報
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUnivHtml/info/4535/R110J.html
• [Remarks] 福岡大学研究者情報